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Quantitative reverse transcription-PCR assay with an internal standard for the detection of prostate-specific antigen mRNA

BACKGROUND: Circulating prostate cells can be detected with a reverse transcription-PCR (RT-PCR) assay for prostate-specific antigen (PSA) mRNA. We have developed a new quantitative RT-PCR method for measuring PSA mRNA. METHODS: The method uses a PSA-like internal standard (IS) mRNA that is added into the sample at the beginning of the RNA extraction and coamplified by RT-PCR with the PSA in the s

Development of Advanced Furnace Control Algorithm

In the continuous work on improving product quality and reducing energy consumption at The Danish Steel Works Ltd. the importance of optimal furnace operation has been recognised in many different contexts. Therefore a joint project with the Department of Automatic Control at Lund Institute of Technology has been started with the purpose of developing a novel slab temperature control algorithm for

Inte fel med genpatenten

Det finns två huvudargument mot patent på gener: Det är oetiskt att patentera mänskliga substanser och organismer och skyddet för angelägen forskning begränsas. Ofta framförs de bägge invändningarna samtidigt. En etiskt resonerande pro-life falang har invändningar mot forskning som rör livets villkor medan de tekniskt orienterade forskarna tvärtom vill förhindra att patentskydd sätter käppar i hju