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Uptake of N-15-labelled alanine, ammonium and nitrate in Pinus sylvestris L. Ectomycorrhiza growing in forest soil treated with nitrogen, sulphur or lime

The uptake of N-15-labelled alanine, ammonium and nitrate was studied in ectomycorrhizal morphotypes of intact Pinus sylvestris seedlings. PCR-RFLP analysis of the ITS-region of fungal rDNA was used to identify the morphotypes. Seedlings were grown in forest soil collected at an experimental site in southern Sweden. The treatments compared were a control, N fertilisation (600 kg N ha(-1) as urea),

Parental stress in families of 2 year old extremely low birthweight infants

OBJECTIVE: To compare the parental stress in the families of 2 year old extremely low birthweight (ELBW) infants with that in control families, and to compare the stress of mothers with that of fathers. METHODS: The study population included all parents of ELBW infants (birth weight < 1000 g and gestational age at least 22 gestational weeks) born between 1 January 1996 and 31 December 1997 in Hels

DNA and cationic surfactants at solid surfaces

The behavior of DNA at different types of interfaces and how the formed structures DNA at an interface could be affected by cationic surfactant has been studied. Particular attention was paid to how the type of cationic surfactant and DNA (single or double stranded and length) affects the compaction/decompaction of DNA. Adsorption of DNA-cationic surfactant complexes is determined by a delicate ba

Amperometric biosensors for detection of sugars based on the electrical wiring of different pyranose oxidases and pyranose dehydrogenases with osmium redox polymer on graphite electrodes

Electrical wiring of different types of pyranose oxidase (P20) (fungal wild type, recombinant wild type with a hexahistidine tag, mutant form E542K with a hexa-histidine tag) from Trametes multicolor, and recombinant P20 from Coriolus sp. overexpressed in Escherichia coli as well as of pyranose dehydrogenase (PDH) from Agaricus meleagris and Agaricus xanthoderma with an osmium redox polymer (poly(

The spondylolytic vertebra and its adjacent segment. Mobility measured before and after posterolateral fusion

STUDY DESIGN: By using roentgen stereophotogrammetric analysis in six patients having tantalum indicators implanted at a preoperative external fixation test, the mobility in the spondylolytic lumbosacral level and its adjacent segment could be studied before fusion and during the course of postoperative fusion consolidation. OBJECTIVE: To study the mobility effects on the segment adjacent to a lum

Green Concurrent Engineering: a model for DFE management programs

Popular Abstract in Swedish Miljöhänsyn vid produktutveckling hanteras alltmer inom ramen för arbetet i certifierbara miljöledningssystem exempelvis enligt den internationella standarden ISO 14001. Detta innebär att industrin i allt högre grad sätter mål och delegerar ansvar för en kontinuerlig förbättring av produkters miljöprestanda inom ramen för speciella miljöledningsprogram för produktutveckEnvironmental considerations in product development are increasingly dealt with in the framework of certified environmental management systems such as ISO 14001. This allows industry to set objectives and assign responsibility for continuous improvement for DFE activities and environmental performance of products in DFE management programs. This thesis presents a general model for DFE management p

Mild head injuries: Impact of a national strategy for implementation of management guidelines

Background. A national survey in 1996 showed insufficient routines for management of patients with mild head injuries in Norwegian hospitals. Since then, the Scandinavian Guidelines for Management of Mild Head Injuries have been published. Methods. A cross-sectional questionnaire survey of management practice was performed in all 59 hospitals in 2002. We compared the results with figures from 1996

Nutrient requirements of lactococci in defined growth media

Many attempts have been made for the last six decades to design defined media for species of the lactococcus group. The general outcome of the studies suggests that this group is heterogeneous with respect to specific requirements for nutrients. Lactococcal species are limited in various metabolic pathways. Early attempts to trace the required nutrients were not always successful because of the po

Antimicrobial activities of heparin-binding peptides.

Antimicrobial peptides are effector molecules of the innate immune system. We recently showed that the human antimicrobial peptides alpha-defensin and LL-37 bind to glycosaminoglycans (heparin and dermatan sulphate). Here we demonstrate the obverse, i.e. structural motifs associated with heparin affinity (cationicity, amphipaticity, and consensus regions) may confer antimicrobial properties to a g

Distinct roles of PKC isoforms in NCAM-mediated neurite outgrowth

The role of protein kinase C (PKC) isoforms in the neural cell adhesion molecule (NCAM)-mediated neurite outgrowth was tested using a co-culture system consisting of fibroblasts with or without NCAM expression upon which either primary cerebellar granular neurones (CGN) or pheochromocytoma (PC12-E2) cells were grown. The latter transiently expressed various PKC isoforms and domains derived from se

Prevalence and risk of pulmonary embolism in patients with intracardiac thrombosis: a population-based study of 23,796 consecutive autopsies

Aims White right intracardiac thrombosis (IT) is a potential cause of pulmonary embolism (PE) similar to that of stroke in left-sided IT, its prevalence and prognostic significance has not been studied in the general population. The aim of this study was to assess the age- and gender-specific prevalence of IT and its relation to PE in a population-based autopsy cohort. Methods and Results Between

Stimulation of muscarinic receptors induces expression of individual fos and jun genes through different transduction pathways

The transduction pathways coupling muscarinic receptors to induction of fos and jun genes were investigated in neuroblastoma SH-SY5Y cells. Stimulation with carbachol induced expression of c-fos, fosB, c-jun, junB, and junD. This effect was abolished by pretreatment with atropine, indicating an involvement of muscarinic receptors. These genes were also induced by activation of protein kinase C wit

Kaernefeltia, a new lichen genus in the Parmeliaceae.

The new cetrarioid genus, Kaernefeltia Thell & Goward is described to accommodate Cetraria californica and C. merrillii, both of which are characterized by small, broadly clavate asci, slightly capitate paraphyses, disc-bar-shaped pycnoconidia and several distinctive fatty acids.

Osseointegration of titanium implants in the tibia. Electron microscopy of biopsies from 4 patients

We studied the ultrastructure of bone tissue around implants of pure titanium inserted into the tibia in 4 patients with arthrosis or rheumatoid arthritis. Three main appearances of the interface were noted. First, a close contact between titanium and calcified bone with living osteocytes inside the newly-formed bone was observed in all samples. Secondly, a close contact was also seen between the

Model atmospheres and spectra: The role of dust

Brown dwarf atmospheres form molecules, then high temperature condensates (corundum, titanates, silicates, and iron compounds), and then low temperature condensates (ices) as they cool down over time. These produce large opacities which govern entirely their spectral energy distribution. Just as it is important to know molecular opacities (TiO, H2O, CH4, etc.) with accuracy, it is imperative to un