A polyacrylamide gel crosslinked with allyl-b-cyclodextrin can be used repeatedly for several weeks for the separation of DNA fragments, since bubbles are not generated during a run. Allyl-b-cyclodextrin can easily be synthesized in one step from allylglycidylether and b-cyclodextrin. The plate numbers for DNA fragments, up to about 1500 bp, are high: for the 21 separation of pBR322/HaeIII fragmen

Analysis of prospectively collected data in a national register.

PURPOSE: To evaluate the use of x-ray imaging performed with a high-power laser system in biologic and medical studies. MATERIALS AND METHODS: A compact terawatt laser system based on chirped pulse amplification in titanium-doped sapphire was used. X rays were generated by irradiating a high-atomic-number target (tantalum). RESULTS: When photons with an energy below 10 keV were removed with use of

Background Studies in many countries have reported higher lung cancer incidence and mortality in individuals with lower socioeconomic status. Methods To investigate the role of smoking in these inequalities, we used data from 391 251 participants in the European Prospective Investigation into Cancer and Nutrition study, a cohort of individuals in 10 European countries. We collected information on

This is an exploratory study investigating potential effects of emotional valence in images and their influence on conversation in the presence of the images. We used latent-semantic analysis to generalize valence ratings of Swedish words to a corpus of spoken conversations. Each utterance in the conversation was given a valence rating, which represented how emotionally positive or emotionally neg

The membrane lipid-anchored glypicans (heparan sulfate proteoglycans) are present in both vertebrates and invertebrates and serve as important modulators of growth factors and morphogens during development. Their core proteins are similar and consist of a large N-terminal domain comprising 14 evolutionary conserved cysteines and a C-terminal stalk carrying the heparan sulfate side-chains and the l

Objectives and design. There are conflicting data on gender differences in survival among heart failure (HF) patients. We prospectively assessed gender differences in survival among 930 consecutive patients (464 [49.9%] women, mean age 76.1 +/- 10.1 years), admitted to hospital with suspected or diagnosed HF. Results. Overall, women had lower unadjusted mortality hazard ratio (HR) than men: HR 0.8

The murine chemokine receptor 2 (mCXCR2) controls resistance to urinary tract infection. We have previously shown that mCXCR2 knockout mice develop severe acute pyelonephritis and renal tissue damage with sub-epithelial neutrophil entrapment. In this study we examined the relative importance of neutrophil- and epithelial-specific mCXCR2 expression for bacterial clearance in bone marrow chimeric mi

AIMS/HYPOTHESIS: This study aimed to examine hand disorders, symptoms, overall hand function, activities of daily living (ADLs), and life satisfaction in elderly men with type 2 diabetes mellitus (DM), impaired glucose tolerance (IGT), and normal glucose tolerance (NGT). METHODS: Subjects were interviewed and evaluated with a battery of clinical and laboratory tests, including hand assessment, and

In this paper I argue that Small pro is not needed as a representative for null subjects in languages like Italian, Spanish, Standard Arabian etc. Instead I propose the Person Phrase hypothesis, which claims that agreement originates as the head of the subject argument. this argument is analyzed as PersP, seen as an expanded DP, [PersP Agr DP]. Agr is a bound morpheme, and thus cannot survive with

BACKGROUND: Local tumour destruction has been shown to give rise to changes in immunocompetent cells. The aim of this study was to describe the effect of interstitial laser thermotherapy (ILT) of breast carcinoma in the tumour and in regional lymph nodes. METHODS: Seventeen women that underwent radical surgical excision after non-radical ILT were studied. ILT was performed at a steady-state temper