Stable Homogeneous Gel for Molecular-Sieving of DNA Fragments in Capillary Electrophoresis
A polyacrylamide gel crosslinked with allyl-b-cyclodextrin can be used repeatedly for several weeks for the separation of DNA fragments, since bubbles are not generated during a run. Allyl-b-cyclodextrin can easily be synthesized in one step from allylglycidylether and b-cyclodextrin. The plate numbers for DNA fragments, up to about 1500 bp, are high: for the 21 separation of pBR322/HaeIII fragmen