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Retrospective diagnostics of congenital cytomegalovirus infection performed by polymerase chain reaction in blood stored on filter paper

Capillary blood samples from 63 infants collected 3-7 days after birth, and thereafter stored on filter papers for 12-18 y, were tested for the presence of CMV DNA by the polymerase chain reaction (PCR) method. Of 16 infants with proven congenital CMV infection (positive virus isolation test in urine sampled within 1 week of age), 13 (81%) had a positive CMV PCR test and 3 (19%) a negative PCR tes

ADP Acting on P2Y13 Receptors Is a Negative Feedback Pathway for ATP Release From Human Red Blood Cells.

Red blood cells may regulate tissue circulation and O-2 delivery by releasing the vasodilator ATP in response to hypoxia. When released extracellularly, ATP is rapidly degraded to ADP in the circulation by ectonucleotidases. In this study, we show that ADP acting on P2Y(13) receptors on red blood cells serves as a negative feedback pathway for the inhibition of ATP release. mRNA of the ADP recepto

Perchlorate stimulates insulin secretion by shifting the gating of L-type Ca2+ currents in mouse pancreatic B-cells towards negative potentials

The effects of the chaotrophic anion perchlorate (ClO4-) on glucose-induced electrical activity, exocytosis and ion channel activity in mouse pancreatic B-cells were investigated by patch-clamp recordings and capacitance measurements. ClO4- stimulated glucose-induced electrical activity and increased the action potential frequency by 70% whilst not affecting the membrane potential when applied in

A revision of the Afrotropical genus Dhatrichia Mosely, 1948 (Trichoptera, Hydroptilidae).

Five previously described species of Dhatrichia Mosely, 1948 are recognized: D. inasa Mosely, 1948 from Yemen; D. bipunctata Statzner, 1977 from Zaire; D. lerabae (Gibon, Guenda & Coulibaly, 1994) comb. n. from Burkina Faso and Ghana; and D. cinyra Wells & Andersen, 1995 and D. divergenta Wells & Andersen, 1995 from Tanzania. A sixth species D. feredougoubae Gibon, 1987 from the Ivory

Theoretical Investigation into Longitudinal Dispersion in Natural Rivers — A Scaling Dispersion Model

Using a broad variety of scientific methods this dissertation systematically investigates the fundamental mechanisms underlying the dispersion processes in natural rivers and their mathematical descriptions in a geometrical framework. Emphasis is on the geometrical variations and irregularities of a natural river and their scales. Analysis starts with the integral scale and the Fickian dispersion

Metoder för riskanalys - Fuktsäkerhet i byggnader

I denna skrift ges en introduktion till riskanalys och riskbedömning vid fuktberäkningar. Vid riskbedömningar kan man ta hänsyn till spridningar i materialegenskaper och omgivande klimatfaktorer. I skriften diskuteras hur man ska välja material - och klimatdata i olika situationer och hur olämpliga val kan ge en betydande över- eller underskattning av risken för ogynsamma fuktförhållanden. Data ka

Gallium phosphide nanowires as a substrate for cultured neurons

Dissociated sensory neurons were cultured on epitaxial gallium phosphide (GaP) nanowires grown vertically from a gallium phosphide surface. Substrates covered by 2.5 mu m long, 50 nm wide nanowires supported cell adhesion and axonal outgrowth. Cell survival was better on nanowire substrates than on planar control substrates. The cells interacted closely with the nanostructures, and cells penetrate

Ekonomens många ansikten - ekonomer i kommuner, landsting och regioner

Varför avbilda ekonomer och hur går det till? Den här studien är en uppföljning, upprepning och utvidgning, av studien ”Ekonom i sikte – bilder av ekonomen i Sveriges kommuner och landsting” som presenterades vid millennieskiftet (Ramberg 2000). Nu, sex år senare, har samma frågor ställts till en långt större population. Ambitionen har varit heltäckande; ett bekvämare representativt urval har inte

GAD65 autoantibody epitopes in adult patients with latent autoimmune diabetes following GAD65 vaccination

Aims Subcutaneous injection of recombinant human GAD65 (rhGAD65) in patients with latent autoimmune diabetes in adults (LADA) correlates with an increase in C-peptide levels. In this study we analysed the effect of rhGAD65 administration on the GAD65-specific autoimmune response. Methods Longitudinal serum samples obtained from LADA patients (n = 47) who received 4, 20, 100 or 500 mu g alum-formul

Intestinal trauma, Analysis of 101 cases

Intestinal injuries sustained by 101 Swedish patients during the period 1950-1979 are reviewed. The abdominal trauma was blunt in 78 cases and penetrating in 23. Small-bowel and mesenteric, but not large-bowel, injuries showed increasing frequency. This was associated with rising numbers of motorcar accidents. Injuries to other abdominal organs were found in 56 of the 101 patients, particularly in

Type I IFN signaling is crucial for host resistance against different species of pathogenic bacteria

It is known that host cells can produce type I IFNs (IFN-alpha beta) after exposure to conserved bacterial products, but the functional consequences of such responses on the outcome of bacterial infections are incompletely understood. We show in this study that IFN-alpha beta signaling is crucial for host defenses against different bacteria, including group B streptococci (GBS), pneumococci, and E

Neuropeptide Y and seizures: effects of exogenously applied ligands

The endogenous NPY system in the brain is centrally involved in seizure regulation. The present paper reviews the evidence that exogenously applied NPY receptor ligands can inhibit epileptic seizures in various rodent in vitro and in vivo models. Agonists at Y2 and/or Y5 receptors and antagonists at Y1 receptors appear to inhibit seizures, depending on the seizure model studied. Although progress