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The genomic characterization of the strain of light cytopathic effect by nucleotide sequence is presented. It was possible to sequence 90% of the strain of light cytopathic effect. A close relationship with Coxsackie A9 was observed. The greatest mutations occurred in the region of the genome that codifies for the structural proteins. Therefore, it may be considered as a variant of Coxsackie A9 ne

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During the epidemic neuropathy occurred in Cuba from 1992 to 1993, viral isolations antigenically connected with Coxsackie viruses were obtained from the cerebrospinal fluid of patients. Virological, epidemiological, toxicologic, nutritional, immunological and histopathological investigations were made. Though the disease was related to toxic and nutritional factors, it has been impossible to iden

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A series of experiments was made at the Virology Department of the "Pedro Kourí" Institute of Tropical Medicine aimed at obtaining new evidences on the possible antigenic relations existing between the viruses isolated from patients with epidemic neuropathy and the structures of the human nervous system. According to the results it may be finally inferred that the persistence and/or autoimmunity m

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An increase of the cases of viral meningoencephalitis was reported in our country from October to December, 1995. 23 faeces specimens were received from 2 provinces aimed at obtaining the viral isolation by their inoculation in cell culture and in newborn mice at the same time in order to compare the sensitivity of both systems in isolation. A higher number of isolations was obtained in cell cultu

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The polymerase chain reaction (PCR) was developed in order to identify the respiratory syncytial virus by using the reference strain. The high sensitivity and specificity obtained show the PCR utility for detecting the RSV genoma and its application on the diagnosis.

Molecular characterization of an outbreak of respiratory syncytial virus (subgroup A) in Havana, Cuba, by monoclonal antibodies and restriction mapping (N gene)

Twenty-one respiratory syncytial virus (RSV) strains isolated from one outbreak in Havana, Cuba (1994 to 1995), were analyzed to determine their relatedness. All isolated strains were classified as subgroup A by monoclonal antibodies. Of 21 RSV strains examined, 20 were classified as having restriction pattern NP4 and only 1 was classified as having restriction pattern NP5.

Analysis of respiratory syncytial virus in clinical samples by reverse transcriptase-polymerase chain reaction restriction mapping

The aim of this study was to develop a polymerase chain reaction (PCR) for the detection of respiratory syncytial virus (RSV) genomes. The primers were designed from published sequences and selected from conserved regions of the genome encoding for the N protein of subgroups A and B of RSV. PCR was applied to 20 specimens from children admitted to the respiratory ward of "William Soler" Pediatric

Fat Words or Useful Concepts? Consensus and Variation in Conceptions of Problematic Drinking in Contemporary Sweden

The aim of this study was to bring to light the meanings attributed to four concepts denoting problematic drinking—“alcoholism,” “alcohol dependence,” “alcohol misuse,” and “risky drinking”—and to investigate whether individuals’ preferences for using these concepts vary depending on their demographic characteristics, their stakeholder group membership, and their own drinking habits. These questio

A Variational Approach to Solitary Gravity–Capillary Interfacial Waves with Infinite Depth

We present an existence and stability theory for gravity–capillary solitary waves on the top surface of and interface between two perfect fluids of different densities, the lower one being of infinite depth. Exploiting a classical variational principle, we prove the existence of a minimiser of the wave energy E subject to the constraint I= 2 μ, where I is the wave momentum and 0 < μ< μ, where μ is

A numerical framework for heat transfer and pressure loss estimation of matrix cooling geometry in stationary and rotational states

This study conducts an investigation and feasibility study on different Reynolds numbers (6000–12000) and Rotation numbers (0.05–0.25) in a matrix cooling geometry. An intended geometry that can be used in gas turbine blades is provided based on flow and heat transfer performance given in these Reynolds and rotation number ranges for stationary and rotational state and then compared with experimen

Characterization of molecular properties of wheat starch from three different types of breads using asymmetric flow field-flow fractionation (AF4)

In this study, molecular properties of wheat starch from three different types of breads were analyzed using asymmetric flow field-flow (AF4) connected to multi-angle light scattering (MALS) and differential refractive index (dRI) detectors. This analysis allowed the determination of molecular properties, i.e. molar mass (M), root-mean-square radius (rrms), apparent density (ρapp) and conformation

Risks and opportunities associated with decarbonising Rotterdam's industrial cluster

The Port of Rotterdam is an important industrial cluster, comprising mainly oil refining, chemical production and power generation. In 2016, the port's industry accounted for 19% of the Netherlands’ total CO2 emissions. The Port of Rotterdam Authority is aware that the cluster is heavily exposed to future decarbonisation policies, as most of its activities focus on trading, handling, converting an

Human action recognition in table-top scenarios : An HMM-based analysis to optimize the performance

Hidden Markov models have been extensively and successfully used for the recognition of human actions. Though there exist wellestablished algorithms to optimize the transition and output probabilities, the type of features to use and specifically the number of states and Gaussian have to be chosen manually. Here we present a quantitative study on selecting the optimal feature set for recognition o

Gradient-enhanced particle filter for vision-based motion capture

Tracking of rigid and articulated objects is usually addressed within a particle filter framework or by correspondence based gradient descent methods. We combine both methods, such that (a) the correspondence based estimation gains the advantage of the particle filter and becomes able to follow multiple hypotheses while (b) the particle filter becomes able to propagate the particles in a better ma

Single view motion tracking by depth and silhouette information

In this work1 a combination of depth and silhouette information is presented to track the motion of a human from a single view. Depth data is acquired from a Photonic Mixer Device (PMD), which measures the time-of-flight of light. Correspondences between the silhouette of the projected model and the real image are established in a novel way, that can handle cluttered non-static backgrounds. Pose i

Using Hidden Markov Models for recognizing action primitives in complex actions

There is biological evidence that human actions are composed out of action primitives, like words and sentences being composed out of phonemes. Similarly to language processing, one possibility to model and recognize complex actions is to use grammars with action primitives as the alphabet. A major challenge here is that the action primitives need to be recovered first from the noisy input signal

The meaning of action : A review on action recognition and mapping

In this paper, we analyze the different approaches taken to date within the computer vision, robotics and artificial intelligence communities for the representation, recognition, synthesis and understanding of action. We deal with action at different levels of complexity and provide the reader with the necessary related literature references. We put the literature references further into context a