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Quantification of Phagocytosis Using Flow Cytometry

Phagocytosis is relevant for many research fields and is often measured as a functional outcome. However, accurate quantification can be challenging, and many researchers find it difficult to study in a robust manner. There are many ways to measure phagocytosis, but what is often overlooked is the importance of experimental design and how the analysis is planned and performed. Experimental factors

Measurement of Antibody Binding Affinity on Bacterial Surfaces Using Flow Cytometry

Antibody binding to bacterial surfaces plays a crucial role in immunity, and a key characteristic of this protein-protein interaction is the binding affinity. Determining the affinity of an antibody binding to its antigen is the first step in predicting the function in a physiological environment where other competing protein interactions may be present. Antibody-antigen affinity is often evaluate

Century-long butterfly range expansions in northern Europe depend on climate, land use and species traits

Climate change is an important driver of range shifts and community composition changes. Still, little is known about how the responses are influenced by the combination of land use, species interactions and species traits. We integrate climate and distributional data for 131 butterfly species in Sweden and Finland and show that cumulative species richness has increased with increasing temperature

Did lockdowns work? : The verdict on Covid restrictions

The purpose of this systematic review and meta-analysis is to determine the effect of lockdowns, also referred to as ‘Covid restrictions’, ‘social distancing measures’ etc., on COVID-19 mortality based on available empirical evidence. We define lockdowns as the imposition of at least one compulsory, non-pharmaceutical intervention (NPI). We employ a systematic search and screening procedure in whi

Involving Members of the Public to Develop a Data Collection App for a Citizen Science Project on Housing Accessibility Targeting Older Adults

Background: While citizen science approaches are emerging within both social and health sciences, projects aimed at improving the living conditions of older adults remain rare. To enable forward-looking housing provision for the ageing population, valid and detailed information is needed on environmental barriers in the housing stock. Moreover, to promote active ageing and avoid involuntary moves

Older women with a low pension, living in Sweden: Strategies to age in place and thoughts about future housing

Financial security influences good quality housing, health, and longevity, but few recent studies of the financial aspects of aging in place have been published. We explored strategies used to age in place and thoughts about future housing among women aged 65 and older living with a low pension in Sweden. We conducted semi-structured qualitative interviews with 13 women aged 65 and older with a lo

"Nit allein den rechtglaubigen, sonder auch den irrigen : Two Sixteenth-Century German Catholic Prayer Books as Tools of Re-Catholicisation"

This article presents two German Catholic prayer books written by the two sixteenth-century priests Johann Faber OP and Peter Michael Brillmacher SJ – known for their catechetical and apologetical work in areas of confessional division. Adding to the claims by early twentieth-century researchers that these books were used for “resisting and combating Protestantism,” I argue that they were tools fo

Bacterial Pathogenesis : Methods and Protocols

This detailed volume presents a diverse set of methodological approaches designed to improve our understanding of bacterial infections from a wide range of bacterial species. Beginning with biofilms and subcellular compartments, the book explores transcriptional analysis, methods for studying plasmid dynamics, and tools for phylogenetic analysis of bacterial genomes, as well as bacterial effector

Birth of the Sleepless Land : The Arrival of the Sugar Beet and Some Ethnological Observations on the Steam Plough and its Fossil Mode of Operation in Scandinavian Agriculture ca. 1880

In this paper, I track the arrival of the sugar beet in Scandinavia and explore the technical changes, ecological implications and new social arrangements that followed in its wake. Based on ethnographic records and other historical material dealing with agricultural practices, I argue that an ethnological view that takes the agrarian landscape and the organisation of social life together as one a

Exoproducts of the Most Common Achromobacter Species in Cystic Fibrosis Evoke Similar Inflammatory Responses In Vitro

Achromobacter is a genus of Gram-negative rods, which can cause persistent airway infections in people with cystic fibrosis (CF). The knowledge about virulence and clinical implications of Achromobacter is still limited, and it is not fully established whether Achromobacter infections contribute to disease progression or if it is a marker of poor lung function. The most commonly reported Achromoba

Novel and unusual genes for nitrogen and metal cycling in Planctomycetota- and KSB1-affiliated metagenome-assembled genomes reconstructed from a marine subsea tunnel

The Oslofjord subsea road tunnel is a unique environment in which the typically anoxic marine deep subsurface is exposed to oxygen. Concrete biodeterioration and steel corrosion in the tunnel have been linked to the growth of iron- and manganese-oxidizing biofilms in areas of saline water seepage. Surprisingly, previous 16S rRNA gene surveys of biofilm samples revealed microbial communities domina

Trophic rewilding can expand natural climate solutions

Natural climate solutions are being advanced to arrest climate warming by protecting and enhancing carbon capture and storage in plants, soils and sediments in ecosystems. These solutions are viewed as having the ancillary benefit of protecting habitats and landscapes to conserve animal species diversity. However, this reasoning undervalues the role animals play in controlling the carbon cycle. We

Reindeer control over shrubification in subarctic wetlands : spatial analysis based on unoccupied aerial vehicle imagery

Herbivores can exert a controlling effect on the reproduction and growth of shrubs, thereby counter-acting the climate-driven encroachment of shrubs in the Arctic and the potential consequences. This control is particularly evident in the case of abundant herbivores, such as reindeer (Rangifer tarandus tarandus), whose grazing patterns are affected by management. Here, we tested how different rein

Almost-Hermitian random matrices and bandlimited point processes

We study the distribution of eigenvalues of almost-Hermitian random matrices associated with the classical Gaussian and Laguerre unitary ensembles. In the almost-Hermitian setting, which was pioneered by Fyodorov, Khoruzhenko and Sommers in the case of GUE, the eigenvalues are not confined to the real axis, but instead have imaginary parts which vary within a narrow “band” about the real line, of

Generalized precursor prediction boosts identification rates and accuracy in mass spectrometry based proteomics

Data independent acquisition mass spectrometry (DIA-MS) has recently emerged as an important method for the identification of blood-based biomarkers. However, the large search space required to identify novel biomarkers from the plasma proteome can introduce a high rate of false positives that compromise the accuracy of false discovery rates (FDR) using existing validation methods. We developed a