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Functional role of extracellular vesicles and lipoproteins in the tumour microenvironment

Cancer can be regarded as an invasive organ that exhibits unique plasticity provided by coordinated, cancer cell-stromal cell communication in the tumour microenvironment. Typical stress factors in the tumour niche, such as hypoxia and acidosis, are major drivers and modulators of these events. Recent findings reveal an important role of extracellular vesicles and lipoproteins in cancer cell adapt

Total internal reflection fluorescence microscopy to study GLUT4 trafficking

Total internal reflection fluorescence (TIRF) microscopy is a powerful method that allows examination of plasma membrane close events in real time. The last decade, the method has successfully been used to explore GLUT4 translocation in adipocytes. Here, we describe the procedure for studying GLUT4 trafficking using TIRF microscopy in isolated primary adipocytes.

Comparison of hospital-based and hospital-based home care at diabetes onset in children

The aim was to compare two different regimens for children diagnosed with type 1 diabetes, hospital-based care and hospital-based home care (HBHC), in terms of the child's metabolic control, episodes of severe hypoglycaemia, the disease's impact on family and the parents' health-related quality of life, one year after diagnosis. The study had a randomised controlled design and included 60 children

Expression and purification of rat glucose transporter 1 in Pichia pastoris

Large amounts of pure and homogenous protein are a prerequisite for several biochemical and biophysical analyses, and in particular if aiming at resolving the three-dimensional protein structure. Here we describe the production of the rat glucose transporter 1 (GLUT1), a membrane protein facilitating the transport of glucose in cells. The protein is recombinantly expressed in the yeast Pichia past

Glucose transport activity measured in giant vesicles

Incorporation of membrane proteins and internal reporter systems directly into giant vesicles, during their formation from a hydrogel surface, has emerged as a promising new concept in membrane protein characterization. Here, we provide the detailed protocol for a glucose transporter activity assay based on giant vesicles containing a fluorescent enzyme-linked reporter system internally. This assa

Writing intervention in university students with normal hearing and in those with hearing impairment : Can observational learning improve argumentative text writing?

Observational learning has shown to be a successful intervention for writing. Up until now, however, studies have only been performed with normal-hearing participants, usually highschool or university students. Additionally, there have been conflicting results in whether subjective text quality correlates with one or more objectively measured text characteristics. In this study, we measured the ef

Retrieval of daily gross primary production over Europe and Africa from an ensemble of SEVIRI/MSG products

The main goal of this paper is to derive a method for a daily gross primary production (GPP) product over Europe and Africa taking the full advantage of the SEVIRI/MSG satellite products from the European Organization for the Exploitation of Meteorological Satellites (EUMETSAT) sensors delivered from the Satellite Application Facility for Land Surface Analysis (LSA SAF) system. Special attention i

Parent–infant interactions looking for instances of volitional social gaze versus reflexive gaze – an observational study

Very early precursors of disrupted social behaviours are significant to understanding the possibility of mitigating or changing behaviours through interventions. Spontaneous play situations between infant and parent in two groups of infants aged 8.5–9 months were observed. First, a large number of videos were analysed to develop an observational schedule of play behaviour. Second, 135 videos were

Fördjupad uppföljning av Flagghusen : Studie av inneklimat, ventilationssystem och vädringsbeteende

In new built apartments buildings with modern mechanical supply and exhaust ventilation, window opening was reported by a majority of the residents. A follow‐up study of the physical indoor environment and the perceived environment by the residents was therefore performed. The follow‐up study is included in a project which also comprises of a follow‐up of the energy use and factors related to this

Decoding of individual finger movements from surface EMG signals using vector autoregressive hierarchical hidden Markov models (VARHHMM)

In this paper we present a novel method for predicting individual fingers movements from surface electromyography (EMG). The method is intended for real-time dexterous control of a multifunctional prosthetic hand device. The EMG data was recorded using 16 single-ended channels positioned on the forearm of healthy participants. Synchronously with the EMG recording, the subjects performed consecutiv

Impact of activated sludge ozonation on filamentous bacteria viability and possible added benefits

Ozone was applied to return activated sludge in full-scale to study how ozone impacts filamentous bacteria viability (Live/Dead®). Additionally, the ozonated sludges were subjected to anaerobic digestion trials and analysis of micropollutants. Ozone treatment (3-4.8 g O3/kg TSS) improved the settling properties of the sludge by lowering the diluted sludge volume with 7-35%. Ozone inactivated filamOzone was applied to return activated sludge in full-scale to study how ozone impacts filamentous bacteria viability (Live/Dead®). Additionally, the ozonated sludges were subjected to anaerobic digestion trials and analysis of micropollutants. Ozone treatment (3-4.8 g O3/kg TSS) improved the settling properties of the sludge by lowering the diluted sludge volume with 7-35%. Ozone inactivated filam

Application of ozone in wastewater treatment : For mitigation of filamentous bulking sludge & reduction of pharmaceutical discharge

Wastewater treatment with activated sludge is the most common way of treating wastewater. The process relies on bacteria converting nutrients, BOD and COD into biomass, CO2 and N2. Even though the configuration of activated sludge processes and wastewater treatment plants varies there are common problems which are shared. Filamentous bulking sludge is one problem that is widespread and disturbs th

Current opportunities for clinical monitoring of axonal pathology in traumatic brain injury

Traumatic brain injury (TBI) is a multidimensional and highly complex disease commonly resulting in widespread injury to axons, due to rapid inertial acceleration/deceleration forces transmitted to the brain during impact. Axonal injury leads to brain network dysfunction, significantly contributing to cognitive and functional impairments frequently observed in TBI survivors. Diffuse axonal injury

Association of common genetic variants related to atrial fibrillation and the risk of ventricular fibrillation in the setting of first ST-elevation myocardial infarction

Background: Cohort studies have revealed an increased risk for ventricular fibrillation (VF) and sudden cardiac death (SCD) in patients with atrial fibrillation (AF). In this study, we hypothesized that single nucleotide polymorphisms (SNP) previously associated with AF may be associated with the risk of VF caused by first ST-segment elevation myocardial infarction (STEMI). Methods: We investigate

Towards gene-and gender-based risk estimates in Lynch syndrome; Age-specific incidences for 13 extra-colorectal cancer types

Background:In Lynch syndrome, inherited mismatch repair (MMR) defects predispose to colorectal cancer and to a wide spectrum of extra-colorectal tumours. Utilising a cohort study design, we aimed to determine the risk of extra-colorectal cancer and to identify yet unrecognised tumour types.Methods:Data from 1624 Lynch syndrome mutation carriers in the Danish hereditary non-polyposis colorectal can

BRCAsearch : written pre-test information and BRCA1/2 germline mutation testing in unselected patients with newly diagnosed breast cancer

Purpose: To evaluate a simplified method of pre-test information and germline BRCA1/2 mutation testing. Methods: In a prospective, single-arm study, comprehensive BRCA1/2 testing was offered to unselected patients with newly diagnosed breast cancer at three hospitals in south Sweden (BRCAsearch, ClinicalTrials.gov Identifier: NCT02557776). Pre-test information was provided by a standardized invita

The effects of ADHD on cognitive performance

Background: Attention-deficit hyperactivity disorder (ADHD) is a common and impairing neurodevelopmental disorder. The Cambridge Neuropsychological Test Automated Battery (CANTAB) is a computerized test battery with standardized procedures and solid psychometric properties targeting multiple neuropsychological functions. Aims: The aim of this study was to look at the effects of ADHD on cognitive p