Studies of Mechanical Properties and Intracellular Ca2+ Transients in Frog Single Muscle Fibres
Intracellular Ca2+ transients, monitored by the calcium-sensitive dye, fluo-3, were studied during isometric twitches and tetani in intact single muscle fibres of the frog (sarcomere length, 2.2 mm; temperature, 2-5 or 10-12 ºC). The Ca2+ transient during twitch stimulation was very brief when compared with the isometric force. In a population of fibres studied, a greater amplitude of the Ca2+ tr
