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Adapting Cancer Cell Lines to a Fully Defined Medium

Lately, there has been a world-wide increase in the awareness of animal cruelty e.g. in the entertainment business, meat industry, in the scientific community, and among people in general. This has called for a scrutiny of how we treat animals and in the scientific community it has stimulated research to reduce animal experiment. Experimental systems usually considered animal cruelty free, like ce

Translational control of the spliceosome under oncogenic stress

Splicing in cancer Proteins are functional molecules responsible for every biochemical process that sustain living organisms. However, how genetic information is expanded to generate amazing diversity of proteins during development and in cancer remains still mostly unresolved. The mechanism we have to account for this diversity is known as splicing. Information-containing RNA molecules, copies

Your thinking matters: Promoting climate-responsible attitudes and behaviour using the choice blindness manipulation

This pre- and post-measure interventional study attempted to create a positive attitude shift in environmental attitudes of 80 students using the choice blindness (CB) manipulation. It is a novel approach that first manipulates the participants’ answers and secondly asks the subjects to justify the answer they did not choose, to elicit attitude change. The second condition utilised a persuasive te

Perioder, punkter och parenteser. Om att översätta långa meningar, avvikande interpunktion och ständiga inskott i en kortroman av Giorgio Bassani

Denna magisteruppsats inom översättarutbildningen bygger på min översättning till svenska av en skönlitterär text skriven av den italienske författaren Giorgio Bassani. Uppsatsen inleds med en presentation av författaren och hans verk och följs av en källtextanalys som följer valda delar av Hellspong och Ledin, Vägar genom texten (1997). Därefter kommer mina överväganden inför översättningen och k

Exposure of zinc oxide nanoparticles on lung epithelium: A comparison between submerged and air-liquid interface cultures

Zinc oxide nanoparticles (ZnO NPs) are often used in sunscreen and other cosmetic products with sun protection. They are also candidate to be used for various biological applications such as drug delivery, or in material science in for example solar cells. ZnO NPs are produced as liquid suspensions but also as dry powders, which increases the risk of exposure via the air. In this master’s thesis,

Levande historia: kolonialism, exceptionalism och komplicitet. En diskursanalys av svenska historieläroböcker

This paper explores discourses about European colonialism and particularly Sweden's colonial legacy in Swedish upper secondary school history textbooks. All three textbooks are currently in use and are based on the latest school curricula from 2011. The theoretical framework used to analyze the textbooks consists of post-colonial feminist theories combined with a post structuralist perspective

Gender, Drugs and Techno music: Investigation of the motivation of drug consumption among raving communities in Skåne region

Rave parties are referring to legal, illegal and semi-legal dance parties organized in various venues with the main focus on electronic dance music (EDM) performed by the DJ. These types of parties are typically described through strong community ties, which form the rave culture, and demolition of hierarchal structures among the participants, which allows the transgressive force to exist in this

Perfectionism, rumination and psychological distress in academic settings

Trait perfectionism has been linked with repetitive negative cognitions, which have been shown to influence psychological distress. This study examined if experiencing evaluation threat in the form of a demanding scholarship status will have any impact on the relationships between these factors. Sixty-seven (67.2% female) university students filled out self-report questionnaires on perfectionism,

Translation tools and retention of word knowledge: A comparison of traditional and modern translation tools

This thesis compares digital and physical dictionaries with each other. Whether digital dictionaries were faster to use and if words were better retained when using a physical dictionary were the specific points of comparison. Based on previous studies It was predicted that physical dictionaries would indeed be better to use if the user wanted to retain knowledge of a word, and that digital dictio

An Introduction to Dirichlet Series

En funktion som har fått mycket uppmärksamhet bland matematiker är den så kallade Riemanns zeta-funktion, som definieras med hjälp av en summa av oändligt antal termer, en så kallad serie: zeta(s) = 1 + 1/2^s + 1/3^s + 1/4^s + ... Här är s=a+ib en komplex variabel med en reell del a och en imaginär del, där i har egenskapen i^2=-1. Värdet på den här serien kommer givetvis bero på vilket värde pWe establish the central convergence properties of ordinary Dirichlet series, including the classical result by Bohr, providing uniform convergence of the series where it has a bounded analytic continuation. We also derive a lower bound for the supremum of Dirichlet polynomials using Kronecker's theorem, of which we see one proof. With this knowledge and some probability theory we can follow t

Investigations into the role of DNAJ Proteins as modulators of amyloid protein aggregation

Implication of DNAJ co-chaperones in Neurodegenerative diseases General improvements of human life conditions at different levels translate into an increasingly growing life span expectancy of the earth population. This, however comes with the cost of an increase in neurodegenerative diseases which broadly increases in frequency with age. Most age related brain diseases such as Parkinson’s dis

Worst Credible Process Fire: Experiences from the Norwegian Petroleum Industry

The term Worst Credible Process Fire (WCPF) was introduced to the Norwegian Petroleum Industry as a complement to the probabilistic approach to define design loads for offshore facilities. Currently there are mainly two documents about the WCPF being the SINTEF report (2017) and the definition in NORSOK S-001 (2018). The WCPF concept have only been used within the industry for a few years and comb

The role of variation in the filaggrin gene for the uptake of chemicals via the skin

The filaggrin gene and the dermal absorption of chemicals Filaggrin is a monomeric protein highly expressed in the outer layer of the epidermis and important for building up the skin barrier. About 7-10% of the European popula-tion have a loss-of-function mutation (so called null mutation) in the filaggrin gene (FLG), which leads to a reduction or loss of the filaggrin protein. The aim of this

Evaluating the effects of an orally administered novel probiotic in mice

A new probiotic for the cure of diabetes Metabolic diseases such as obesity and type 2 diabetes are becoming increasingly common worldwide and are major causes of disability and death indicating an urgent need for interventions. Emerging evidence points to a central role of gut microbiota in metabolism, for example blood glucose metabolism and appetite regulation. Furthermore, intervention with

Construction and characterization of ApoE3-GFP as a tool to study ApoE trafficking

Lysande protein ska hjälpa spåra ändringar vid alzheimers sjukdom Demens är ett syndrom som påverkar en stor del av populationen vid högre ålder. Syndromet yttrar sig genom att påverka personers kognitiva förmågor, huvudsakligen minnet. Frekvensen kommer fortsätta att öka med den åldrande populationen. Den vanligaste och mest välkända varianten av demens är alzheimers sjukdom. Som alla vet är deApolipoprotein E4 (ApoE4) is the biggest genetic risk factor for Alzheimer’s disease. While it is known to transport cholesterol to neurons the localization of the protein has not yet fully been mapped. Levels of amyloid precursor protein (APP) and its cleavage product amyloid beta (Aβ), the main component of amyloid plaques, in transfected cells were determined using western blot. Green fluoresce

Improving the security of exposed safety critical systems using SDN

The purpose of this thesis is to study if software defined networks (SDN) can function as a second layer of defence in safety critical sensor networks. SDNs are controlled and topologically defined by a logically centralised control unit. The centralised control logic makes it possible to control the behaviour of the network, and react to network events. In this thesis we examine if and how SDNs c