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Continuous full filling capillary electrochromatography: Chromatographic performance and reproducibility

Continuous full filling capillary electrochromatography with nanoparticles as pseudostationary phase interfaced with electrospray ionisation mass spectrometric detection was used for reversed phase separations with very high separation efficiency. Several batches of nanoparticles were synthesised and their electrochromatographic performance were evaluated. Different parameters, such as repeatabili

Bladder volume alters cholinergic responses of the isolated whole mouse bladder

Purpose: The isolated bladder expresses autonomous activity, which may contribute to the generation of lower urinary tract sensation or pathophysiology. We evaluated how the effect of a cholinergic agonist on autonomous activity alters with increasing volume and in the presence of substances known to modulate functional bladder capacity. Materials and Methods: The bladder of 22 adult female C57 bl

A deterministic and probabilistic model for oilspill fires in nuclear power plants: a final report to the Swedish Atomic Energy Board

A deterministic and probabilistic model for oilspill fires in nuclear power plant compartments has been developed. It's objective is to predict whether certain components in the compartment will cease to function as a result of the fire and to give the probability of failure. Results are presented for several scenarios in two compartments. The model has been implemented in the computer code OSFIC,

Efficient blind system identification of non-Gaussian autoregressive models with HMM modeling of the excitation

We have previously proposed a blind system identification method that exploits the underlying dynamics of non-Gaussian signals in [Li and Andersen, "Blind identification of Non-Gaussian Autoregressive Models for Efficient Analysis of Speech Signals," Proceedings of the International Conference on Acoustics, Speech and Signal Processing (ICASSP), May 2006, vol. 1, pp. I-1205-1-1208]. The signal mod

Do leukotrienes increase cell viability in human intestinal epithelial cells?

In this preliminary report we present data showing that leukotrienes increase the baseline cell viability in human intestinal epithelial cells and that LTB4 partially reverses the morphological hallmarks of non-necrotic cell death induced by the COX-2 specific inhibitor NS-398. The proposed signaling mechanisms regulating these events are summarized in fig. 3. Please view the work on LT signal tra

Benzodiazepine prescribing patterns in a high-prescribing Scandinavian community

Sales statistics indicate large variations in benzodiazepine consumption between the Scandinavian countries: the current difference between Denmark (highest) and Sweden (lowest) is almost two-fold. There are also large within-country variations: e.g. benzodiazepine sales in the Swedish city of Helsingborg, which is close to Denmark, were at the average Danish level and were the highest in Sweden.

A high-resolution core-level study of Ni-catalyzed absorption and desorption of hydrogen in Mg-films

Ultra-thin magnesium and magnesium-nickel films grown on Mo(110) under ultra-high vacuum (UHV) conditions are hydrogenated at room temperature with atomic H and studied by high-resolution core-level photoelectron spectroscopy (PES). For the Mg-film a layer of hydride is formed at the Mg/Mo interface and a sub-mono layer of hydride is formed at the surface, while an intermediate region remained non

Competitive Na+ and Rb+ binding in the minor groove of DNA

Sequence-dependent coordination of alkali ions to the nucleotide bases in the minor groove of AT-tract B-DNA has recently been inferred from X-ray crystallography, solution NMR and computer simulations. Here, we present new Na-23 and Rb-87 magnetic relaxation dispersion (MRD) data that demonstrate competitive and long-lived binding of Na+ and Rb+ ions in the minor groove of the B-DNA duplex [d(CGC

Nyretorik och muslimsk vanitaspredikan: Perelman läst i skuggan av al-Hurayfish

Perelman läst i skuggan av al-Hurayfish. Chaïm Perelman bidrog till att förnya intresset för retoriken som ett ämne värt seriös uppmärksamhet och hans så kallade nya retorik har påverkat mycket av den retorikforskning som bedrivs idag. I följande artikel diskuterar islamologen Philip Halldén den nya retoriken mot bakgrund av en medeltida muslimsk predikosamling, tillskriven al-Hurayfish. Frågan är

Contrasting views over a hybrid complex: Between speciation and evolutionary "dead-end"

The Squalius alburnoides complex, of hybrid origin, comprises diploid, triploid and tetraploid forms and has a widespread distribution in the Iberian waters. The southern populations of this complex, sympatric with S. pyrenaicus, show high genetic variability, diversity of forms and reproductive modes which create pathways that may allow for the establishment of a new species in the future. Here w

Morphometric study of mesenteric and renal arteries in spontaneously hypertensive rats

Arteries from spontaneously hypertensive rats (SHR), stroke-prone spontaneously hypertensive rats (SHRSP) and Wistar-Kyoto controls (WKY) were fixed by immersion and plastic embedded. The media cross-sectional area and the length of the internal elastic membrane were measured. The ratio between media thickness and internal radius was then calculated for a standardized condition, implying a perfect

Increased urinary excretion of free N-acetylneuraminic acid in thirteen patients with Salla disease

Thirteen severely retarded patients with Salla disease, a new type of lysosomal storage disorder, have been studied biochemically. All patients excreted approximately ten times more free sialic acid than normal individuals. The isolated sialic acid was characterized by paper chromatography, thin-layer chromatography, optical rotation, 13C and 1H nuclear magnetic resonance spectroscopy, and mass sp

Influx and efflux of potassium rubidium-86 in UV-C irradiated guard of Vicia-Faba.

Effects of ultraviolet radiation (UV) (254 nm) on isolated guard cells of Vicia faba (cv. Long pod) have been investigated with regards to fluxes of 86Rb+. UV treatment inhibited influx by about 80%, while it increased efflux by up to 350%. Compartmental analysis showed a highly significant difference (p .ltoreq. 0.005) between the slow leakage components of the UV-treated cells (t1/2 = 5.8 min) a

An algorithm including results of gray-scale and power Doppler ultrasound examination to predict endometrial malignancy in women with postmenopausal bleeding.

OBJECTIVE: To determine if power Doppler ultrasound examination of the endometrium can contribute to a correct diagnosis of endometrial malignancy in women with postmenopausal bleeding and endometrium >/= 5 mm. METHODS: Eighty-three women with postmenopausal bleeding and endometrium >/= 5 mm underwent gray-scale and power Doppler ultrasound examination using predetermined, standardized settings. S

A comparison of the inner-sphere reorganization energies of cytochromes, iron-sulphur clusters, and blue copper proteins

Inner-sphere reorganization energies have been calculated for a number of models of six-coordinate iron porphyrins (with varying axial ligands), using the density functional B3LYP method. If the axial ligands are uncharged, the reorganization energy is very low, 5-9 kJ/mol. If one of the axial ligands is charged, the reorganization energy is higher, 20-47 kJ/mol, but such sites are normally not us

Osteopontin is an adhesive factor for myeloma cells and is found in increased levels in plasma from patients with multiple myeloma

Background and Objectives. Osteopontin (OPN) is a non-collagenous matrix protein produced by various cells including osteoblasts, osteoclasts and several types of tumor cells. It is involved in a number of physiologic and pathologic events including adhesion, angiogenesis, apoptosis, inflammation, wound healing and tumor metastasis. We wanted to investigate the potential role of OPN in multiple my