Physiological Engineering of Xylose Utilisation by Recombinant Saccharomyces cerevisiae
Xylitol production by recombinant, XYL1-expressing Saccharomyces cerevisiae was investigated in fed-batch fermentation using different cosubstrates for growth, and generation of reduced cofactors and maintenance energy. Xylose was converted into xylitol with 1:1 yield. Using ethanol as cosubstrate, the yield of xylitol on ethanol and the specific xylitol productivity decreased with increasing aera