Single cell kinetics of RNAi mediated knockdown
Eukaryotic cells employ the RNA interference (RNAi) machinery in the cytosol to downregulate gene expression. Short 21-nucleotide microRNAs (miRNAs) associate with the so-called RNA induced silencing complex (RISC) and hybridize with the target gene’s mRNA, preventing it from being translated into protein. Employing the RNAi machinery with synthetic small interfering RNAs (siRNAs) that mimics the