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Simultaneous quantification of human glandular kallikrein 2 and prostate-specific antigen mRNAs in peripheral blood from prostate cancer patients

We present a multiplexed and internally calibrated quantitative reverse transcription-PCR (QRT-PCR) assay to detect human glandular kallikrein 2 (hK2) and prostate-specific antigen (PSA) transcripts in blood samples from healthy subjects and prostate cancer (PC) patients. The assay detected 50 copies of hK2 and PSA mRNA, and 1 PSA- and 10 hK2-expressing LNCaP cells in the presence of 2.5 x 10(6) P

Effects of pH on Helicobacter pylori binding to human gastric mucins: Identification of binding to non-MUC5AC mucins

Helicobacter pylori causes gastritis, peptic ulcer disease and gastric cancer. The microbe is found in the gastric mucus layer where a pH gradient ranging from acidic in the lumen to neutral at the cell surface is maintained. The aim of the present study was to investigate the effects of pH on H. pylori binding to gastric mucins from healthy individuals. At pH 3, all strains bound to the most char

Common principles of sensory encoding in spinal reflex modules and cerebellar climbing fibres.

An important step towards understanding the function of olivo-cerebellar climbing fibres must be to clarify what they signal. We suggest that climbing fibres projecting to paravermal cerebellum mediate highly integrated sensorimotor information derived from activity in spinal withdrawal reflex modules acting on single forelimb muscles. To test this hypothesis, cutaneous nociceptive receptive field

Bilden av Fakiren

Genomgång av illustrationer till Axel Wallengrens "fakiriska" verk och hur dessa förhåller sig dels till själva texterna och dels till dessas upphovsman.

Transmission of BSA during Cross-flow Microfiltration: Influence of pH and Salt Concentration

In many downstream processes microfiltration (MF) is used as the first step to separate the micro-organisms from the product-containing broth. The transmission of proteins through these MF membranes is of great importance, especially when a protein is the desired product. Usually, the largest fraction of the proteins retained in the membrane during the separation of micro-organisms is retained in

Grundvattenvärme : kvarteret Tärnan i Landskrona

Rapporten redovisar fältförsök, uppföljning och termohydraulisk utvärdering av en experimentbyggnadsanläggning i kvarteret Tärnan i Landskrona. En låg energiförbrukning eftersträvas genom överglasad gata m.m.

Progression of retinopathy after improved metabolic control in type 2 diabetic patients. Relation to IGF-1 and hemostatic variables

OBJECTIVE: To determine the impact of improved glycemic control on the development and progression of retinopathy after the institution of insulin therapy in patients with type 2 diabetes and to assess the relation to IGF-1 and hemostatic variables. RESEARCH DESIGN AND METHODS: In a prospective observational study, 45 type 2 diabetic patients were examined at baseline and 1, 3, 6, 12, and 24 month

Bet v 1 homologues in strawberry identified as IgE-binding proteins and presumptive allergens

Background: No strawberry allergen has so far been identified and characterized. Methods: Serum samples were collected from patients with a suggestive case history of adverse reactions to strawberry and other fruits. Extracts from fresh and frozen strawberries were analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), Western blotting and mass spectrometry. Patient blo

Vegetation history and lake-level changes from the Younger Dryas to the present in Eastern Pyrenees (France): pollen, plant macrofossils and lithostratigraphy from Lake Racou (2000 m a.s.l.)

A multi-proxy palaeoecological investigation including pollen, plant macrofossil, radiocarbon and sedimentological analyses, was performed on a small mountain lake in the Eastern Pyrenees. This has allowed the reconstruction of: ( 1) the vegetation history of the area based on five pollen diagrams and eight AMS(14)C dates and ( 2) the past lake-level changes, based on plant macrofossil, lithologic

Clinical characteristics and primary treatment of prostate cancer in Sweden between 1996 and 2005 - Data from the national prostate cancer register in Sweden

Objective. The incidence of prostate cancer is rising rapidly in Sweden and there is a need to better understand the pattern of diagnosis, tumor characteristics and treatment. Material and methods. Between 1996 and 2005, all new cases of adenocarcinoma of the prostate gland were intended to be registered in the National Prostate Cancer Register (NPCR). This register contains information on diagnos

Ska generna styra vad vi äter?

This article gives an overview of some ethical problems raised in connection to nutritional genomics, especially the use of knowledge from nutrigenomics as a basis for personalised nutrition after a genetic test.

En moral för hela världen? : en analys av Hans Küngs Projekt Weltethos

Popular Abstract in Swedish Avhandlingen analyserar den schweiziske teologen Hans Küng och hans arbete med att utforma en universell moral. Enligt Küng kan världsreligionerna ligga till grund för en moral som kan sträcka sig över alla ideologiska, kulturella och religiösa gränser. Denna moral, som vilar på världsreligionerna, kan sedan ligga till grund för världspolitiken och garantera att relatioThis dissertation analyzes the work on universal morality by the Swiss theologian Hans Küng. According to Küng, a universal morality can be formed out of the world's religions that can reconcile ideological, cultural and religious differences. This morality can then form the basis for international policy and politics and guarantee that relations among states are peaceful. Küng's project focuses

A calibration system for soil carbon dioxide efflux measurement chambers: Description and application

Comparisons have revealed large discrepancies among the many methods for measuring soil CO2 efflux indicating the need for an absolute calibration of methods. This study presents a calibration system, constructed to imitate an area of soil, and its application to two different chamber systems for the measurement Of Soil CO2 efflux: one open and one closed dynamic. Air rich in CO2 was allowed to di