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Acetic acid as a decontamination method for sink drains in a nosocomial outbreak of metallo-β-lactamase-producing Pseudomonas aeruginosa

Background Pseudomonas aeruginosa may colonize water systems via biofilm formation. In hospital environments, contaminated sinks have been associated with nosocomial transmission. Here we describe a prolonged outbreak of a metallo-β-lactamase-producing P. aeruginosa (Pae-MBL) associated with sink drains, and propose a previously unreported decontamination method with acetic acid. Aim To describe a

Transmitter-specific subsets of sensory elements in the prosobranch osphradium.

The osphradium is a putative chemosensory organ of aquatic molluscs. Previously, we identified two distinct types of primary sensory neurons in the osphradial ganglion of freshwater pulmonates, one immunoreactive to leucine-enkephalin (LEnk-ir) and another to FMRFamide (FMRFa-ir). In addition, NADPH diaphorase (NADPHd)-positive elements apparently producing nitric oxide (NO) were demonstrated in t

Sensory structures associated with the cuticle of Hutchinsoniella macracantha (Crustacea, Cephalocarida)

The body cuticle of the cephalocarid crustacean Hutchinsoniella macracantha Sanders, 1955, carries two types of small setae which differ in their external shape and number of ciliated sensory cells. It also has two types of pores, one being a gland opening, the other containing the tip of a cilium. The setae and the pore type containing a ciliated sensory cell are considered chemosensory organs.

Serotonin- and FMRFamide-immunoreactive nerve elements in the chiton, Lepidopleurus asellus (Mollusca, Polyplacophora)

The distribution of serotonin-like and FMRFamide-like immunoreactive (5HT-ir and FMRFa-ir, respectively) neurons in the nervous system of the chiton Lepidopleurus asellus (Mollusca, Polyplacophora) was studied using an immunocytochemical technique. The neurons were distributed in characteristic patterns in the central nervous system, the 5HT-ir neurons predominating in the ventral (pedal) cords an

Immunolabeled neuroactive substances in the osphradium of the pond snail, Lymnea stagnalis.

The osphradium of molluscs is assumed to be a sensory organ. The present investigation in Lymnaea stagnalis has established two ultrastructurally different types of dendrites in the sensory epithelium. Cells immunoreactive to leucine-enkephalin and FMRFamide send processes to the sensory epithelium. These neurons of the osphradial ganglion are thus considered to be part of the sensory system, as a

Patterns of enkephalin immunolabeling in the pulmonate snail Cepea nemoralis and related molluscs.

In the land pulmonate snailCepaea nemoralis, immunocytochemical localization of methionine- and leucine-enkephalin-like substances was demonstrated in specific populations of brain neurons. Methionine-enkephalin reactivity is also present in the peripheral nervous system as (i) abundant axonal projections of central neurons to the sole of the foot, and (ii) sparse local neurons of the head and man

Is nitric oxide (NO) produced by invertebrate neurons?

NADPH-DIAPHORASE (NADPHd) is known to be identical to nitric oxide (NO) synthase in the mammalian nervous system, and is therefore used as a marker of NO-producing neurones. Using the histochemical reaction for NADPHd, we searched for such neurones in a selection of invertebrates. Special emphasis was given to molluscs. No selective neuronal staining was found in representatives of coelenterates,

Eliciting parents' individual requirements for an inclusive digital school system

Parents often have a busy time sorting out their life puzzles, including getting information about their children's activities in school. More and more communication between teachers and parents take place via digital school systems. It can be hard for parents to find the information they are looking for and the teacher decides when information is sent and what communication method to use. All par

The light-yield response of a NE-213 liquid-scintillator detector measured using 2–6 MeV tagged neutrons

The response of a NE-213 liquid-scintillator detector has been measured using tagged neutrons from 2 to 6 MeV originating from an Am/Be neutron source. The neutron energies were determined using the time-of-flight technique. Pulse-shape discrimination was employed to discern between gamma-rays and neutrons. The behavior of both the fast (35 ns) and the combined fast and slow (475 ns) components of

A systems analysis of biodiesel production from wheat straw using oleaginous yeast : process design, mass and energy balances

Background: Biodiesel is the main liquid biofuel in the EU and is currently mainly produced from vegetable oils. Alternative feedstocks are lignocellulosic materials, which provide several benefits compared with many existing feedstocks. This study examined a technical process and its mass and energy balances to gain a systems perspective of combined biodiesel (FAME) and biogas production from str

Global warming potential and energy analysis of second generation ethanol production from rice straw in India

The environmental sustainability of cellulosic ethanol production from rice straw in India is conducted using life cycle assessment (LCA). Greenhouse gas (GHG) emissions, net energy ratio (NER) and net energy balance (NEB) are studied for ethanol production system using two diverse pretreatment technologies, i.e. dilute acid (DA) and steam explosion (SE) followed by separate hydrolysis and ferment

The test beamline of the European Spallation Source – Instrumentation development and wavelength frame multiplication

The European Spallation Source (ESS), scheduled to start operation in 2020, is aiming to deliver the most intense neutron beams for experimental research of any facility worldwide. Its long pulse time structure implies significant differences for instrumentation compared to other spallation sources which, in contrast, are all providing short neutron pulses. In order to enable the development of me

A new biological recovery approach for PHA using mealworm, Tenebrio molitor

Bacterial polyhydroxyalkanoates (PHA) are expensive partly due to the recovery and purification processes. Thus, many studies have been carried out in order to minimize the cost. Here we report on the use of mealworm, which is the larva of mealworm beetle (Tenebrio molitor) to recover PHA granules from Cupriavidus necator. Mealworms were shown to readily consume the freeze-dried C. necator cells a

From genome-wide arrays to tailor-made biomarker readout – Progress towards routine analysis of skin sensitizing chemicals with GARD

Allergic contact dermatitis (ACD) initiated by chemical sensitizers is an important public health concern. To prevent ACD, it is important to identify chemical allergens to limit the use of such compounds in various products. EU legislations, as well as increased mechanistic knowledge of skin sensitization have promoted development of non-animal based approaches for hazard classification of chemic

The dynamics of platelet activation during the progression of streptococcal sepsis

Platelets contribute to inflammation however, the role of platelet activation during the pathophysiological response to invasive bacterial infection and sepsis is not clear. Herein, we have investigated platelet activation in a mouse model of invasive Streptococcus pyogenes infection at 5,12, and 18 hours post infection and correlated this to parameters of infection. The platelet population in ex-