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Histidine protonation states are key in the LigI catalytic reaction mechanism

Lignin is one of the world's most abundant organic polymers, and 2-pyrone-4,6-dicarboxylate lactonase (LigI) catalyzes the hydrolysis of 2-pyrone-4,6-dicarboxylate (PDC) in the degradation of lignin. The pH has profound effects on enzyme catalysis and therefore we studied this in the context of LigI. We found that changes of the pH mostly affects surface residues, while the residues at the active

The Effect of Coupling Memory and Block Length on Spatially Coupled Serially Concatenated Codes

Spatially coupled serially concatenated codes (SC-SCCs) are a class of spatially coupled turbo-like codes, which have a close-to-capacity performance and low error floor. In this paper, we perform a comprehensive design space exploration, revealing different aspects of SC-SCCs and discussing various design trade-offs. In particular, we investigate the impact of coupling memory, block length, decod

Dispersal and spatiotemporal distribution of Protapion fulvipes in white clover fields : implications for pest management

Yield loss caused by insect pests remains a substantial problem in agriculture. Chemical control, with potential negative effects on non-target organisms, is still the main tool for pest management. For pest species with limited dispersal capacity, rotation of the crop in time and space has potential as an alternative management measure. This is particularly important in organic farming, where mos

Evolution of conventional communication : A cross-cultural study of pantomimic re-enactments of transitive events

This study addresses the postulate of non-conventionality of pantomime, inherent in pantomimic scenarios of language origin. Since lack of semiotic conventions does not preclude micro-conventions resulting from cultural differences, pantomimes should be easier to interpret when the actor and recipient share the same culture than between two different cultures. In the study, Italian and Polish amat

Towards Fully Pipelined Decoding of Spatially Coupled Serially Concatenated Codes

Having close-to-capacity performance and low error floor, even for small block lengths, make spatially coupled serially concatenated codes (SC-SCCs) a very promising class of codes. However, classical window decoding of SC-SCCs either limits the minimum block length or requires a large number of iterations, which increases the complexity and constrains the degree to which an SC-SCC decoder archite

Feasting with Buddhist Women : Food Literacy in Religious Belonging

This ethnographic study shows that women’s knowledge and practices involving food in Japanese Buddhist contexts circulate as gendered currency. It emphasizes how what we term “food literacy” cultivates aesthetic and affective senses of belonging among Buddhist practitioners. We argue that this embodied knowledge helps women negotiate their experiences of Buddhism and show how these experiences art

Investigation on residual stress evolution in nickel-based alloy affected by multiple cutting operations

Machining-induced residual stresses in a part can significantly influence its performance and service time. It is common that the final surface of the part is produced after multiple cuts in the machining process. In multiple cuts, the previous cuts could often generate accumulated strain/stress and temperature on the part surface. These accumulated strain/stress and temperature will be brought to

Early mitochondrial metabolism and dynamics changes as predictors of neurodegeneration and neuronal cell death

Background: Neuronal calcium and ATP homeostasis is regulated through alterations in mitochondria fusion/fission events and anterograde and retrograde transport in neurons. Mitochondrial dynamics form an interactome that ultimately controls mitochondrial quality, quantity and metabolism. Our objective is to show that alterations in this interactome lead to point-of-no-return situations characteriz

Coherent J / ψ and ψ ′ photoproduction at midrapidity in ultra-peripheral Pb–Pb collisions at √sNN=5.02 TeV

The coherent photoproduction of J/ψ and ψ′ mesons was measured in ultra-peripheral Pb–Pb collisions at a center-of-mass energy sNN−−−√ = 5.02 TeV with the ALICE detector. Charmonia are detected in the central rapidity region for events where the hadronic interactions are strongly suppressed. The J/ψ is reconstructed using the dilepton (l+l−) and proton–antiproton decay channels, while for the ψ′ t

Jet energy scale and resolution measured in proton–proton collisions at √s=13 TeV with the ATLAS detector

Jet energy scale and resolution measurements with their associated uncertainties are reported for jets using 36–81 fb−1 of proton–proton collision data with a centre-of-mass energy of s√=13 TeV collected by the ATLAS detector at the LHC. Jets are reconstructed using two different input types: topo-clusters formed from energy deposits in calorimeter cells, as well as an algorithmic combination of c

Hepatic and extrahepatic insulin clearance in mice with double deletion of glucagon-like peptide-1 and glucose-dependent insulinotropic polypeptide receptors

The aim of this study was to investigate whether incretins, at physiological levels, affect hepatic and/or extrahepatic insulin clearance. Hepatic and extrahepatic insulin clearance was studied in 31 double incretin receptor knockout (DIRKO) and 45 wild-type (WT) mice, which underwent an Intravenous Glucose Tolerance Test (IVGTT). A novel methodology based on mathematical modeling was designed to

A methodology for the assessment of compound sea level and rainfall impact on urban drainage networks in a coastal city under climate change

This study seeks to investigate how stormwater drainage systems in coastal cities respond to climate change in terms of simultaneous sea level rise and rainfall. 7.5 years of recorded rainfall and sea level data in the city of Trelleborg in Sweden were used to generate projections of future climate events based on the emission scenario RCP8.5. Twelve scenarios were formulated to represent rain and

Optimization of experimental and computational processes to study mitochondrial trafficking and metabolic biomarkers

Background: The complex polarity of neurons requires specialized mechanisms to allocate sufficient number of mitochondria to neurites and synapses to control local calcium and ATP levels. Deficits in trafficking are directly linked to neurodegeneration. Our objective is to demonstrate that alterations of mitochondrial spatial location and metabolism are interconnected and can lead to point-of-no-r

Critical Review of Electro-kinetic Remediation of Contaminated Soils and Sediments : Mechanisms, Performances and Technologies

Remediation of contaminated soil and sediment is important for improving the eco-environmental quality. Electro-kinetic remediation (EKR) is an environmentally friendly technology to migrate and remove pollutants from the soil and sediment matrix. This paper analyses the mechanism and performance of EKR of heavy metals, organic pollutants, and compound pollutants. Moreover, the effect of optimizin