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Expression of enzymatically active rat liver and human placental catechol-O-methyltransferase in Escherichia coli; purification and partial characterization of the enzyme

To produce sufficient amounts of recombinant catechol-O-methyltransferase (COMT) for structural and functional studies the coding regions of the rat liver and human placental COMT genes have been introduced into a bacterial expression vector pKEX14. Recombinant COMT was produced in Escherichia coli up to 10% of total bacterial protein after the induction of the T7 RNA polymerase gene with isopropy

Aspartic proteinase from barley grains is related to mammalian lysosomal cathepsin D

Resting barley (Hordeum vulgare L.) grains contain acid-proteinase activity. The corresponding enzyme was purified from grain extracts by affinity chromatography on a pepstatin-Sepharose column. The pH optimum of the affinity-purified enzyme was between 3.5 and 3.9 as measured by hemoglobin hydrolysis and the enzymatic activity was completely inhibited by pepstatin a specific inhibitor of aspartic

A pilot study of the predestined planning conditions and the indoor environment

A pilot study has been conducted to find out if and how the building process influences the indoor air or the perception of the indoor air. As the first part of this investigation, a media study of what are considered to be healthy and unhealthy buildings was performed. From this media study two buildings were chosen and their building processes were examined more closely. This was carried out as

Performance of Energy Efficient Windows and Solar Shading Devices

This Licentiate dissertation deals with windows and solar shading devices and how they could be designed and used to save energy for heating and cooling in buildings. Parametric studies using the dynamic energy simulation software ParaSol v 2.0 were performed for different windows and solar shading devices. One study showed that both the cooling load and the annual cooling demand could decreased