We demonstrate that the cccB gene, identified in the Bacillus subtilis genome sequence project, is the structural gene for a 10-kDa membrane-bound cytochrome c551 lipoprotein described for the first time in B. subtilis. Apparently, CccB corresponds to cytochrome c551 of the thermophilic bacterium Bacillus PS3. The heme domain of B. subtilis cytochrome c551 is very similar to that of cytochrome c55

Succinate:quinone reductase is a membrane-bound enzyme of the citric acid cycle and the respiratory chain. Carboxin is a potent inhibitor of the enzyme of certain organisms. The bacterium Paracoccus denitrificans was found to be sensitive to carboxin in vivo, and mutants that grow in the presence of 3'-methyl carboxin were isolated. Membranes of the mutants showed resistant succinate:quinone reduc

Cytochromes of the c type contain covalently bound heme. In bacteria, they are located on the outside of the cytoplasmic membrane. Cytochrome c synthesis involves export of heme and apocytochrome across the cytoplasmic membrane followed by ligation of heme to the polypeptide. Using radioactive protoheme IX produced in Escherichia coli, we show that Bacillus subtilis can use heme from the growth me

The characterization and analytical performance of a tyrosinase graphite-epoxy electrode for the detection of phenolic compounds are described. The biocomposite configuration is based on the entrapment of commercially available tyrosinase in a graphite-epoxy matrix, and the mixing of the resulting conductive epoxy resin with a hardener. The enzyme electrode is mounted as a working electrode in an

Inside-out submitochondrial particles (IO-SMP) were isolated and purified from potato (Solanum tuberosum L. cv.) tubers. When these IO-SMP were incubated with [γ32P]ATP more then 20 proteins became labelled as a result of phosphorylation. The 32P incorporation was stimulated by the oxidizing reagent ferricyanide. Except for a 17 kDa protein which was phosphorylated only in the absence of divalent

Lignin, the second most abundant natural polymer, has emerged as a potential alternative material to petroleum-based chemicals and renewable resource for the production of diverse forms of aromatics, biofuels, and bio-based materials. Thus, it is becoming important to understand its structure and properties to provide key features and insights for better/efficient lignin valorisation. In this work

Aeolian dunes, coversands, glacifluvial deltas and paraglacial mass-movement deposits in Bonäsheden and Skattungheden in Dalarna, central Sweden were dated by optically stimulated luminescence (OSL) on quartz. The OSL ages confirm that the majority of the dunes started forming directly after deglaciation, as suggested by previous investigators. Dune formation seems to have lasted at least 1500 yea

Background and aims Barley kernel based products have been shown to induce benefits on blood glucose regulation, cardio-metabolic risk markers and appetite regulating hormones in a time perspective of 11–16 h after intake. The mechanisms have been assigned to gut fermentation of indigestible carbohydrates. The purpose of the present study was to evaluate if the modulatory effects of barley on mark

An applied kHz optical remote sensing system is implemented to determine the flight direction of an insect with respect to wind direction, and its relative size using near- and shortwave infrared light in situ.

This paper proposes an IV-based panel unit root test that is general enough to accommodate general error serial and cross-section dependence, and a potentially nonlinear deterministic trend function. These allowances make the new test one of the most general around. It is also very simple to implement. Indeed, the IV statistic is asymptotically invariant to not only to all nuisance parameters char

We consider the problem of tool position and orientation state estimation for robot manipulators in workspace by sensor fusion of the internal robot joint measurements with inertial measurement unit data. A prerequisite for this to be successful is accurate calibration of the sensors used. Therefore, we discuss a method for calibration of the sensor with respect to the robot end-effector, which is

To many Swedish citizens, their everyday contacts with the Church of Sweden in matters of civil registration and impediments of marriage created their strongest im pressions of the Church. Thus, as an example of ecclesiastical history of everyday life (Alltagsgeschichte), my study is of great interest from the point of view of Church history. It illustrates how the performance of the civil duties

Factors influencing teachers' selection of content in geography teaching is a fundamental didactic matter.1 The purpose of the present study was to investigate whether Swedish geography teachers' informal and formal experiences have influenced their interest in geography and if so, in what way. The results disclosed that informal experiences like outings, holidays, and childhood memories have a si

Although relatively rare, Huntington’s disease (HD) has fatal consequences. There is no cure for the disease, which leads to an early death. Worldwide, scientists are trying to develop therapeutic methods that could cure the disease, including new molecular gene therapeutic methods. At Lund University, research on HD is now about to step from animal models to trials with humans. The project is spe

Subsurface transport of orthophosphate (Pi) from fertilized agricultural fields to freshwaters may lead to eutrophication, reduced biodiversity and fish kills in inland waters. Reduction of Pi transport by means of filters in drains with Pi sorbing materials (PSMs) may be one way to improve water quality. The aims of this study were to investigate and model the effect of retention time (RT) and in

Phosphorus eutrophication of lakes and streams, coming from drained farmlands, is a serious problem in areas with intensive agriculture. Installation of phosphate (P) sorbing filters at drain outlets may be a solution. The aim of this study was to improve the understanding of reactions involved in P sorption by three commercial P sorbing materials, i.e. Ca/Mg oxide-based Filtralite-P, Fe oxide-bas