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All in the family [Review of the motion picture Requiem]

Reviews the film, Requiem, directed by Hans-Christian Schmid (2006). This award-winning (for both its star, Sandra Hüller, and the film itself) German film is based on the case of Anneliese Michel, a 23-year-old German woman who died in 1976, probably of starvation (she weighed 68 lb. just before her death) and exhaustion, after 67 exorcism rites. Hüller's nuanced performance chills the spine far

On the possibility of using high pressure treatment to modify physico-chemical properties of dietary fibre in white cabbage (Brassica oleracea var. capitata)

The possibility of using high hydrostatic pressure (HHP) to modify the physico-chemical properties of dietary fibre polysaccharides in white cabbage (Brassica oleracea var. capitata) was studied. Two cultivars (Heckla and Predikant) were subjected to high pressure treatment (400 and 500 MPa) at different temperatures (20, 50 and 80 °C). The total dietary fibre (TDF) content was only slightly affec

Electric control of spin polarization orientation in a magnetic-electric barrier structure

We investigate spin-dependent transport properties of two-dimensional electron systems modulated by both the stray field from a ferromagnetic metal (FM) stripe and the electrostatic potential (EP) provided by two normal metal Schottky stripes which sandwich the FM stripe. The EP consists of a single barrier and a single well. By switching the gate voltages applied to the Schottky stripes, the barr

Coupling of ligand binding and dimerization of helix-loop-helix peptides: Spectroscopic and sedimentation analyses of calbindin D-9k EF-hands

Isolated Ca2+-binding EF-hand peptides have a tendency to dimerize. This study is an attempt to account for the coupled equilibria of Ca2+-binding and peptide association for two EF-hands with strikingly different loop sequence and net charge. We have studied each of the two separate EF-hand fragments from calbindin D-9k. A series of Ca2+-titrations at different peptide concentrations were monitor

Nonlinear Pricing under a Balanced-Budget Requirement: The n-Type Case

An economy consisting of n>=2 different types of consumers and one publicly owned natural monopoly is under consideration. The preferences of the consumers are assumed to be linear in money and the demand curves are assumed not to cross. We also suppose that the net utility from consumption is so high that we do not have to consider the individual rationality constraints. Given these assumptions,

Induction of a regulatory phenotype in human CD4(+) T cells by streptococcal M protein

Regulatory T cells (Tregs) participate in the control of the immune response. In the human system, an IL-10-secreting, T regulatory type 1 cell (Tr1)-like subset of Tregs can be induced by concurrent cross-linking of the TCR and CD46 on naive CD4(+) T cells. Because many viral and bacterial pathogens, including the major human pathogen Streptococcus pyogenes, bind to CD46, we asked whether this ba

Orbital and spin contributions to the g tensors in metal nanoparticles

We present a theoretical study of the mesoscopic fluctuations of g tensors in a metal nanoparticle. The calculations were performed using a semirealistic tight-binding model, which contains both spin and orbital contribution to the g tensors. The results depend on the product of the spin-orbit scattering time tau(so) and the mean-level spacing delta, but are otherwise weakly affected by the specif

Harmful azo colorants in leather. Determination based on their cleavage and extraction of corresponding carcinogenic aromatic amines using modern extraction techniques.

This study concerns the possibilities of using microwave-assisted extraction (MAE) or supercritical fluid extraction (SFE) for detection of harmful azo colorants in leather. After degreasing of the leather sample with SFE there follows a reductive cleavage of the azo colorants to their corresponding aromatic amines in the MAE or SFE equipment. The aromatic amines are subsequently extracted using e

HYDRO - A PROGRAM FOR PROTEIN HYDROPATHY PREDICTIONS

VAX/VMS programs for predicting protein hydropathy from amino acid sequence with several parameters are described. The hydropathy propensities of amino acids were normalised to make the different scales and predictions comparable. Another program package can be used to study similarity on the secondary structural level by comparing, for example, hydropathy predictions. These methods can provide va

Assessment of work postures and movements using a video-based observation method and direct technical measurements

The aim was to study postures and movements during repetitive work using video-based observations and direct technical measurements (inclinometers and goniometers). A total of 21 healthy women from a poultry processing plant volunteered. Neck flexion > 20 degrees was registered during 92% of the recorded time with the observation method, while the corresponding value measured with the inclinometer

Transient kinetics of ligand binding and role of the C-terminus in the dUTPase from equine infectious anemia virus

Transient kinetics of the equine infectious anemia virus deoxyuridine 5'-triphosphate nucleotide hydrolase were characterized by monitoring the fluorescence of the protein. Rate constants for the association and dissociation of substrate and inhibitors were determined and found to be consistent with a one-step mechanism for substrate binding. A C-terminal part of the enzyme presumed to be flexible

The cysteine protease inhibitors cystatins inhibit herpes simplex virus type 1-induced apoptosis and virus yield in HEp-2 cells

The role of cystatins in herpes simplex virus (HSV)-induced apoptosis and viral replication has been studied. Human epithelial (HEp-2) cells infected with wild-type HSV-1 (F), with a deletion virus lacking the anti-apoptotic gene Us3 (R7041) or with a deletion virus lacking the anti-apoptotic genes Us3 and ICP4 (d120) were treated with cystatin A, C or D. Cells and culture media were studied at di

Monte Carlo simulation of defect-free cross-linked polyelectrolyte gels

Model systems of cross-linked polyelectrolyte gels were investigated by means of Monte Carlo simulations. The model contained a charged defect-free th ee-dimensional network of a diamond-like topology and explicit counterions. Pressure vs volume relations and chain extensions were determined and compared to those of the corresponding polyelectrolyte solution. The structure of the gel was character