Separation of rat leukocytes by countercurrent distribution in aqueous two-phase systems. II. Subpopulations which mediate selective and nonselective lysis of normal and colon carcinoma target cells in vitro
Spleen cells from WF rats immunized to allogeneic lymphoid cells or to syngeneic colon carcinomas and from unimmunized controls were separated by countercurrent distribution in aqueous two-phase systems. The cells were assayed for cytotoxicity to allogeneic fibroblasts or syngeneic colon carcinoma cells and to syngeneic fibroblasts in a 24-hr 51Cr-release assay. Cells from immunized rats which sel
