Post-translational methylation of lysine side chains is of great importance for protein regulation, including epigenetic control. Here, we present specific 13CHD2 labeling of dimethylated lysines as a sensitive probe of the structure, interactions, and dynamics of these groups, and outline a theoretical and experimental framework for analyzing their conformational dynamics using 1H and 13C CPMG re

Galectins are a medically important family of small, soluble carbohydrate-binding proteins that have been implicated in a wide variety of diseases, and are therefore targets for the development of new drugs. Many X-ray crystal structures of small molecules in complex with galectins have been determined and have given invaluable information on how natural ligands and synthetic inhibitors are recogn

Treatment of haemophilia A/B patients comprises factor VIII (FVIII) or factor IX (FIX) concentrate replacement therapy, respectively. FVIII and FIX activity levels can be measured in clinical laboratories using one-stage activated partial thromboplastin time (aPTT)-based clotting or two-stage chromogenic factor activity assays. We discuss strengths and limitations of these assays, providing exampl

A novel model-independent Lévy imaging method is employed for reconstruction of the elastic pp and pp¯ scattering amplitudes at low and high energies. The four-momentum transfer t-dependent elastic slope B(t), the nuclear phase φ(t) as well as the excitation function of the shadow profile P(b) have been extracted from data at ISR, Tevatron and the LHC energies. We found qualitative differences in

Objectives: With increased long-term survival after traumatic brain injury (TBI), there is a need to understand the life situation many years after the injury. In this study, we have assessed persons on average 16 years after their injury and determined changes over 6 years in overall outcome, living condition, marital status and vocational situation, and in their functioning and disability. Mater

Purpose: Postoperative bleeding after thyroid surgery remains a potentially lethal complication. Outpatient thyroidectomy is an increasing trend in the high volume centers. There is a need to identify risk factors for postoperative bleeding in order to select proper patients for outpatient thyroidectomy. This study aimed to investigate this issue using a national population-based register. Materia

Patient participation in forensic psychiatric settings seems to be complex by nature, and previous studies show that patients rate their participation as lower in this context compared to general psychiatric contexts. Studies on caregivers’ perspective could provide a clearer picture of the components and possibilities of patient participation in forensic psychiatry. The aim of the study is to des

Parental care in birds varies among species and geographic regions. Incubation behavior influences embryonic development rate and varies substantially among species. We studied attendance at the nest by videoing nests or collecting data from the literature for 112 species in north temperate and lowland tropical sites, then associated patterns of incubation on- and off-bouts with species and enviro

Amyloid fibril formation is a hallmark of neurodegenerative disease caused by protein aggregation. Oligomeric protein states that arise during the process of fibril formation often coexist with mature fibrils and are known to cause cell death in disease model systems. Progress in this field depends critically on development of analytical methods that can provide information about the mechanisms an

Efficient intracellular nucleic acid delivery into mammalian cells remains a long-standing challenge owing to poor cell permeability and uptake of naked nucleic acids across the cell membrane and limited cargo stability. Conventional delivery methods have several drawbacks, such as cytotoxicity, limited cell-type applicability, low efficiency, hindrances that limit the potential of oligonucleotide

This paper aims to describe the reasons why abnormal heat transfer occurs under the condition of regenerative cooling applied in scramjet engines. Supercritical n-decane flowing in a horizontal tube under various mass flow rates (G = 0.001, 0.002, 0.003, 0.004 kg/s) and heat fluxes (qw = 100, 200, 300, 400 kW/m2) is investigated by numerical simulations. The mechanisms of the abnormal heat transfe

We use Gaia DR2 astrometric and line-of-sight velocity information combined with two sets of distances obtained with a Bayesian inference method to study the 3D velocity distribution in the Milky Way disc. We search for variations in all Galactocentric cylindrical velocity components (Vφ, VR, and Vz) with Galactic radius, azimuth, and distance from the disc mid-plane. We confirm recent work showin

Welding fumes were recently classified as carcinogenic to humans and worldwide millions work as welders or perform welding operations. The purpose of this study was to identify new biomarkers of welding-induced carcinogenesis. We evaluated a panel of 91 putative cancer-related proteins in serum in a cohort of welders working with mild steel (n = 77) and controls (n = 94) from southern Sweden sampl

Studies of how contextual factors influence teachers’ approaches to teaching have chiefly focused on face-to-face teaching in physical campus contexts. This study advances understanding of how applicable this knowledge is in digital contexts. The study explores how university teachers perceive the differences between teaching in physical campus contexts and the digital teaching contexts found in o

Aims: In deglaciated surfaces, lithology influences habitat development. In particular, serpentinite inhibits soil evolution and plant colonization because of insufficient phosphorus (P) content, among other stressful properties. In nutrient-poor environments, ectomycorrhizal fungi (EMF) play a key role exploring the soil for P beyond the rhizosphere. In this study, we followed the role of EMF in

Ultralight lattice structures exhibit excellent mechanical performance and have been used widely. In structural design, the fundamental frequency is highly important. Therefore, a multiscale topology optimization method was utilized to optimize the fundamental frequency of multimaterial lattice structures in this study. Two types of optimization problems were studied, namely, maximizing the natura

Statistical model results suggest that the declining growth rate from autumn to spring is the key to cause El Niño-Southern Oscillation (ENSO) spring persistence barrier (SPB). Using a dynamical approach, we develop the physical mechanisms responsible for ENSO SPB in the framework of recharge oscillator by adding a seasonally varying Bjerknes (BJ) stability index and linking it with ENSO growth ra

Aims: To predict irreversible reduction in left ventricular ejection fraction (LVEF) during admission for ST-segment elevation myocardial infarction (STEMI) using cardiac magnetic resonance (CMR) in addition to classical clinical parameters. Irreversible reduction in LVEF is an important prognostic factor after STEMI which necessitates medical therapy and implantation of prophylactic implantable c