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Release and cellular contents of pro- and anti-inflammatory cytokines, neutrophilic elastase and secretory leukocyte proteinase inhibitor (SLPI) were measured with enzyme-linked immunosorbent assay in peripheral blood mono- and polymorphonuclear cells stimulated with preopsonized yeast cells or lipopolysaccharide. Tumour necrosis factor alpha (TNFα) was also measured with a bioassay. TNFα producti

Populärvetenskaplig sammanfattningUnder den senaste tiden har det varit ganska populärt att studera smart materia som kan bilda nya strukturer beroende på externa förhållanden. Detta arbete är fokuserat på att studera hur man kan designa sådan ma- teria. Med hjälp av dator simuleringar har vi modellerat partiklar med riktade interaktioner och studerat deras beteenden i bulk.Förhoppningen är att omThere has been an increased interest to design “smart materials” which can self-assemble into complex structures in accordance to external factors such as for example electromagnetic fields. By designing particles with directional interactions one is able to control how particles will interact with each other. By means of both Monte Carlo and Molecular Dynamics simulations we study the interaction

A 24-kDa protein was purified from human neutrophil extracts and shown to be the newly discovered neutrophil gelatinase-associated lipocalin (NGAL), based on structural and immunochemical data. A specific enzyme-linked immunosorbent assay (ELISA) was developed for the determination of NGAL in human plasma and tissue fluids. Normal human plasma contains 72 μg 1-1 of NGAL (range 40-109 μg 1-1) in tw

Neutrophil proteinase 4 (NP4) is a major neutral proteinase of the human polymorphonuclear (PMN) leukocyte, which is present in amounts similar to leukocyte elastase. NP4(3) is a potent, non-specific proteinase, which may degrade structural and soluble proteins in the tissues and body fluids, and it has been implicated as an important pathogenetic factor in lung emphysema. We have studied the rele

Dog polymorphonuclear leukocyte cathepsin G was isolated from a granule extract using a two-step procedure including affinity chromatography on aTrasylol-Sepharose gel and ion-exchange chromatography on a CM 52 column. 22 of the first 24 N-terminal amino acids were determined and showed 83% and 71% identity to those of human and rat cathepsin G, respectively. Total amino-acid composition demonstra

Knowledge of species’ habitat requirements can be gained from studying individual variation in home range size, under the assumption that larger home ranges reflect increased resource needs or decreased habitat quality. We used satellite telemetry to delineate home ranges of South Scandinavian Common Cuckoos (Cuculus canorus) throughout their annual cycle. Annual stage (breeding or non-breeding pe

The spatio-temporal schedule provided by geolocators suggests that a pair of Barn Swallows could have remained together during the non-breeding period. Data from four birds, of which two were a breeding pair, showed winter quarters from West to Central Africa. The tracks of the paired individuals coincided both spatially and temporally at a scale of <200 km throughout the non-breeding period, in c

Although surface integral equations (SIEs) have been extensively used in solving electromagnetic problems of penetrable objects, there are still open issues relating to their application to the Theory of Characteristic Modes. This work demonstrates that when an SIE is used to solve for the characteristic modes (CMs) of a dielectric or magnetic object, the resulting eigenvalues are unrelated to the