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Enzyme conformational alterations detected by partition column chromatography

In this paper, we demonstrate the ability of liquid-liquid partition chromatography (LLPC) to detect conformational alterations occurring in well-characterized enzymes. The conformational changes induced in dehydrogenases such as alcohol dehydrogenase (ADH), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), lactate dehydrogenases (LDH) and malate dehydrogenase (MDH) upon binding of ligand(s) were

Extracellular matrix, esterase and the phytotoxin prehelminthosporol in infection of barley leaves by Bipolaris sorokiniana

Light microscopy and cryo-scanning electron microscopy showed that hyphae of Bipolaris sorokiniana adhered to the wax surfaces of barley leaves by means of an extensive extracellular matrix (ECM). Prehelminthosporol, the major non-host specific phytotoxin formed by B. sorokiniana was immunolocalized in large amounts in the ECM surrounding the hyphae. Similarly, esterase activity involved in degrad

Source breakup dynamics in Au+Au collisions at root s(NN)=200 GeV via three-dimensional two-pion source imaging

A three-dimensional correlation function obtained from midrapidity, low p(T), pion pairs in central Au+Au collisions at root s(NN) = 200 GeV is studied. The extracted model-independent source function indicates a long range tail in the directions of the pion pair transverse momentum (out) and the beam (long). A proper breakup time tau(0) similar to 9 fm/c and a mean proper emission duration Delta

Postural and symptomatic improvement after physiotherapy in patients with dizziness of suspected cervical origin

OBJECTIVE: To assess postural performance in patients with dizziness of suspected cervical origin in whom extracervical causes had been excluded, and to assess the effects of physiotherapy on postural performance and subjective complaints of neck pain and dizziness. DESIGN: Prospective, randomized, controlled trial. SETTING: Primary care centers and a tertiary referral center. PATIENTS AND SUBJECT

Characteristics of cardiac arrest and resuscitation by age group: an analysis from the Swedish Cardiac Arrest Registry

Aim: The objective of this study was to describe patients who experienced an out-of-hospital cardiac arrest (OHCA) by age group. Methods: All patients who suffered from an OHCA between 1990 and 2005 and are included in the Swedish Cardiac Arrest Registry (n = 40,503) were classified into the following age groups: neonates, younger than 1 year; young children, between 1 and 4 years; older children,

Distribution and characterisation of fructan in wheat milling fractions

Structure and health effects of inulin-type fructans have been extensively studied, while less is known about the properties of the graminan-type fructans in wheat. Arabinoxylan (AX) is another important indigestible component in cereal grains, which may have beneficial health effects. In this study, the fructan content in milling fractions of two wheat cultivars was determined and related to ash,

Both common and unique susceptibility genes in different rat strains with pristane-induced arthritis.

Pristane-induced arthritis (PIA) in rats is an animal model for rheumatoid arthritis (RA). We have previously identified seven quantitative trait loci (QTLs), which regulate arthritis development using a cross between the susceptible DA strain and the resistant E3 strain of rats (Pia2-8). In the present study the inbred rat strain LEW.1F was used as the susceptible strain in a cross with the E3 st

The evolution of Holocene coastal dunefields, Jutland, Denmark: A record of climate change over the past 5000 years

Coastal dunefields have developed on the west coast of Jutland in Denmark over the past 5000 years. The dunefields are situated in a temperate climate zone with frequent high energy wind events. Dunefield development was characterized by repeated periods of transgressive dune formation punctuated by periods of dune stabilization and soil formation. The chronology of dunefield evolution is based on

Mechanical design of the recirculating, terminal pumping in the Lund Pelletron, and experimental experience

A recirculating terminal pumping system has been installed in the 3 MV Pelletron tandem accelerator in Lund. An extremly limited space in the high voltage terminal and the absence of electrical power in the terminal, forced us to provide a unique design for the installation and powering of the new pumps. Details of the technical design, as well as experience of the use of the new system for accele

Perceptions of the concept of health among patients in mental health nursing.

Health has been a central concept in nursing science since the 18th century but the holistic concept of health that includes both the body and the soul, still has to be clarified. The concept of health is often unclear and represents an unreachable ideal state that can be hard to use as a realistic goal in nursing care. The aim of this study was to describe how the patient perceives the concept of

Foraging among cannibals and kleptoparasites: effects of prey size on pike behavior

The northern pike (Esox lucius) is an important and selective piscivore that chooses smaller prey than predicted from energy / time budgets. In a laboratory experiment, we investigated pike predatory behavior to explain this selectivity. Northern pike feeding on different prey sizes in aquaria were observed when foraging alone, when in the presence of chemical cues from similar-sized or larger con

Testosterone and diurnal rhythmicity of leptin, TNF-alpha and TNF-II receptor in insulin-resistant myotonic dystrophy patients

OBJECTIVE: To investigate the leptin and TNF systems in relation to testosterone in insulin resistant myotonic dystrophy (DM1) subjects. DESIGN AND SUBJECTS: Fasting morning samples and diurnal sampling during 24 h. Forty-two DM1 subjects (20 women and 22 men; age 41.5 (28.5-58.7)y, body mass index (BMI) 23.3 (18.6-29.2)kg/m(2); median and 10th and 90th percentile, respectively). Fifty healthy vol