Binding, internalization, and degradation of 125I-labeled, antiproliferative, or nonantiproliferative heparan sulfate by human embryonic lung fibroblasts was investigated. Both L-iduronate-rich, antiproliferative heparan sulfate species as well as L-iduronate-poor, inactive ones were bound to trypsin-releasable, cell-surface sites. Both heparan sulfate types were bound with approximately the same

The effects of various glycosaminoglycans (GAGs) on the growth rate of normal fibroblasts and a fibrosarcoma cell line (HT 1080) were examined. Cells were grown in 96-well microplates in the absence or presence of serum mitogens, epidermal (EGF), platelet-derived (PDGF), acidic fibroblast (aFGF), or basic fibroblast growth factor (bFGF). Cell number was measured by using crystal violet to stain ce

BACKGROUND: Clinical outcome of patients with triple-negative breast cancer (TNBC) is highly variable. This study aims to identify and validate a prognostic protein signature for TNBC patients to reduce unnecessary adjuvant systemic therapy.METHODS: Frozen primary tumors were collected from 126 lymph node-negative and adjuvant therapy-naive TNBC patients. These samples were used for global proteom

We recently reported on the development of a 4-protein-based classifier (PDCD4, CGN, G3BP2, and OCIAD1) capable of predicting outcome to tamoxifen treatment in recurrent, estrogen-receptor-positive breast cancer based on high-resolution MS data. A precise and high-throughput assay to measure these proteins in a multiplexed, targeted fashion would be favorable to measure large numbers of patient sa

Estrogen receptor (ER)-positive breast cancer represents the majority (∼70%) of all breast malignancies. In this subgroup of breast cancers, endocrine therapies are effective both in the adjuvant and recurrent settings, although resistance remains a major issue. Several high-throughput approaches have been used to elucidate mechanisms of resistance and to derive potential predictive markers or alt

In a previous study, we detected a significant association between phosphoserine aminotransferase 1 (PSAT1) hyper-methylation and mRNA levels to outcome to tamoxifen treatment in recurrent disease. We here aimed to study the association of PSAT1 protein levels to outcome upon tamoxifen treatment and to obtain more insight in its role in tamoxifen resistance. A cohort of ER positive, hormonal thera

This article addresses the big problems that Socotra island was facing in 2015; hurricanes, and the influence of war in Yemen.

We address a new agricultural policy concern following the decoupling of CAP direct payments in 2005: passive farming, whereby landowners maintain their agricultural area to collect payments without producing commodities. It is claimed that passive farming is hindering agricultural development by 'blocking' access to farmland for expanding farmers. We evaluate the links between the EU's Single Pay

Genetic polymorphisms are powerful model systems to study the maintenance of diversity in nature. In some systems, polymorphisms are limited to female coloration; these are thought to have arisen as a consequence of reducing male mating harassment, commonly resulting in negative frequency-dependent selection on female color morphs. One example is the damselfly Ischnura elegans, which shows three f

This paper proposes a framework for the analysis of technological innovation processes in transnational contexts. By drawing on existing innovation system concepts and recent elaborations on the globalization of innovation, we develop a multi-scalar conceptualization of innovation systems. Two key mechanisms are introduced and elaborated: the generation of resources in multi-locational subsystems

A test of CP invariance in Higgs boson production via vector-boson fusion using the method of the Optimal Observable is presented. The analysis exploits the decay mode of the Higgs boson into a pair of τ leptons and is based on 20.3 fb - 1 of proton–proton collision data at s = 8 TeV collected by the ATLAS experiment at the LHC. Contributions from CP-violating interactions between the Higgs boson

A reduced version of the complex aqueous phase mechanism CAPRAM3.0i (C3.0RED) was used in the regional chemistry transport model COSMO–MUSCAT in a 2-D application. Besides sulfate and nitrate production, the mechanism treats a complex HOx-chemistry, transition metal ion chemistry and organic species up to C4. The effects of the cloud chemistry on the chemical composition of air and particles were

Hexaarylbisimidazole (HABI) is a well established photoinitiator in the graphics industry which undergoes in the absence of other reaction partners a fully reversible homolytic bond cleavage into two lophyl radicals L. Surprisingly we now found oscillations of the radical concentration at low temperature in a solution of o-Cl-HABI in toluene, which is an indication of a more complex photochemistry

Following financialization, there has emerged an understanding of what it implies to be a shareholder based on the shareholder value perception. However, as this shareholder value perception spreads internationally, it clashes with traditional perceptions. In this paper, we apply the language developed by Bourdieu to a Swedish public debate on equal treatment of shareholders in connection with theFollowing financialization, there has emerged an understanding of what it implies to be a shareholder based on the shareholder value perception. However, as this shareholder value perception spreads internationally, it clashes with traditional perceptions. In this paper, we apply the language developed by Bourdieu to a Swedish public debate on equal treatment of shareholders in connection with the

A search for supersymmetry involving the pair production of gluinos decaying via third-generation squarks to the lightest neutralino (χ10) is reported. It uses an LHC proton-proton data set at a center-of-mass energy s=13 TeV with an integrated luminosity of 3.2 fb-1 collected with the ATLAS detector in 2015. The signal is searched for in events containing several energetic jets, of which at least

A measurement of the total pp cross section at the LHC at s=8 TeV is presented. An integrated luminosity of 500 μb−1was accumulated in a special run with high-β⋆beam optics to measure the differential elastic cross section as a function of the Mandelstam momentum transfer variable t. The measurement is performed with the ALFA sub-detector of ATLAS. Using a fit to the differential elastic cross sec