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Calmodulin‐Binding Proteins Within the Slow Phase of Axonal Transport in the Rabbit Vagus Nerve Per Ekstrom and Martin Kanje

Abstract: : Calmodulin‐binding proteins (CBPs) in the rabbit vagus nerve were studied by means of calmodulin‐Sepha‐rose affinity chromatography and polyacrylamide gel electrophoresis. The soluble fraction (105g supernatant) of a nerve homogenate contained four CBPs with molecular weights of 44, 55, 91, and 93 kD, respectively. Slowly transported proteins were recovered in the vagus 3 days after in

Nerve regeneration and serum levels of insulin-like growth factor-I in rats with streptozotocin-induced insulin deficiency

Peripheral nerve regeneration was studied in female Sprague-Dawley rats with streptozotocin-induced insulin deficiency. Nerve regeneration was provoked by a crush lesion on the sciatic nerve 21 days after the streptozotocin injection. The regeneration was assessed by a pinch test at different time-points after injury. The rate ofregeneration in insulin-deficient animals, 2.5 mm/day, was significan

An Overview of Knowledge Extraction Projects in the NLP group at Lund University

In this paper, I describe systems and prototypes we created in the natural language processing group at Lund to extract structured knowledge from text. Starting from syntactic and semantic parsingcomponents,wedevelopedapplicationsthatcanhandlelargecorpora,typicallycomplete Wikipedia versions consisting of millions of documents and process text to identify entities and the relations between them. I

Rational design of a thermostable glycoside hydrolase from family 3 introduces β-glycosynthase activity

The thermostable β-glucosidase from Thermotoga neapolitana, TnBgl3B, is a monomeric three-domain representative from glycoside hydrolase family 3. By using chemical reactivation with exogenous nucleophiles in previous studies with TnBg13B, the catalytic nucleophile (D242) and corresponding acid/base residue (E458) were determined. Identifying these residues led to the attempt of converting TnBgl3B

Insulin and IGF-II, but not IGF-I, stimulate the in vitro regeneration of adult frog sciatic sensory axons

We used the in vitro regenerating frog sciatic nerve to look for effects of insulin and insulin-like growth factors I and II (IGF-I, IGF-II) on regeneration of sensory axons and on injury induced support cell proliferation in the outgrowth region. In nerves cultured for 11 days, a physiological dose (10 ng/ml, ≈ nM) of insulin or IGF-II increased ganglionic protein synthesis (by 20% and 50%, respe

Axonal outgrowth from adult mouse nodose ganglia In vitro is stimulated by neurotrophin-4 in a Trk receptor and mitogen-activated protein kinase-dependent way

The actions of neurotrophic factors on sensory neurons of the adult nodose ganglion were studied in vitro. The ganglia were explanted in an extracellular matrix-based gel that permitted observation of the growing axons. Neurotrophin-4 (NT-4) was a very efficient stimulator of outgrowth of axons from the nodose ganglion and had almost doubled the outgrowth length when this was analyzed after 2 days

Calmodulin and In Vitro Regenerating Frog Sciatic Nerves : Release and Extracellular Effects

Although calmodulin (CaM) is commonly considered to be an intracellular protein, it has been suggested lately that it is released and exerts functions extracellularly. In the present investigation this was studied in in vitro regenerating adult frog (Rana temporaria) sciatic nerves. Using a multi‐compartment incubation chamber, the non‐neuronal cells in the outgrowth region of such nerves were rad

Perceptions of health and risk management among home care workers in Sweden

BACKGROUND: Municipal home care workers provide high-quality services to an increasing proportion of elderly people living in private homes. The work environments and working conditions of these workers vary to a great extent, implying rapid priority-making among both employers and employees to ensure that the work can be performed in a safe way.OBJECTIVES: This study aims to examine home care wor

Child Rights, Classroom and School Management : Change Projects from the International Training Programme Batch 17 - 2012b

Lund University has offered the Sida-financed International Training Programmeon Child Rights, Classroom and School Management since 2003. The programmetargets those in a position from which they can initiate processes of change in theeducation sector in their countries. During the programme all participating teamsinitiate a change project in their respective countries aiming at the realization of

Vad händer egentligen på museerna?

För att museerna ska kunna möta sin publik och representera människor, kulturer och subkulturer med respekt och tolerans, måste den vetenskapliga debatten hållas fri. Dagens överdrivna självkritik och romantiska relativism riskerar att leda till en skev bild av historien. Det skriver Christer Lindberg, professor i socialantropologi, som här ger sin syn på kulturpolitiken och museernas framtid.

Use of explant cultures of peripheral nerves of adult vertebrates to study axonal regeneration in vitro

Explanted preparations of peripheral nerves with attached dorsal root ganglia of adult mammals and amphibia survive for several days in serum-free medium and can be used to study axonal regeneration in vitro. This review outlines the methods which we routinely use and how they may be applied to study different aspects of axonal regeneration. When the peripheral nerves are crushed in vitro, axons r

Okadaic Acid and Cultured Frog Sciatic Nerves : Potent Inhibition of Axonal Regeneration in Spite of Unaffected Schwann Cell Proliferation and Ganglionic Protein Synthesis

Abstract: Okadaic acid (OA) is a frequently used phosphatase inhibitor that by inhibiting dephosphorylation increases the net phosphorylation level in various systems. In the present study OA was used to assess the role of balanced phosphorylation‐dephosphorylation reactions for successful regeneration of peripheral nerves. To achieve this, the effects of OA on phosphorylation levels, neurite outg

Child Rights, Classroom and School Management : Change Projects from the International Training Programme Batch 12 - 2010a

Lund University has offered the Sida-financed International Training Programme on Child Rights, Classroom and School Management since 2003. The programme targets those in a position from which they can initiate processes of change in the education sector in their countries. During the programme all partici¬pating teams initiate a change project in their respective countries aiming at the reali¬zat

In situ observations of the isotopic composition of methane at the Cabauw tall tower site

High-precision analyses of the isotopic composition of methane in ambient air can potentially be used to discriminate between different source categories. Due to the complexity of isotope ratio measurements, such analyses have generally been performed in the laboratory on air samples collected in the field. This poses a limitation on the temporal resolution at which the isotopic composition can be

Life-views of Physiotherapy students compared to medical and nursing students

A life-view is an overall view of man and the world, forming a central value system and giving expression to a fundamental attitude. Research has shown that life-views are established early in childhood, are influenced by social environment and are relatively constant over time. An individual's view of life determines the response to reality and influences decision-making and treatment of other pe

Comparisons of various approaches to low frequency in-situ measurements and corresponding models

New recommendations for environmental noise levels have been issued in Sweden. The permissible levels at facades of new buildings have been increased, which has resulted in a risk for higher indoor low frequency noise levels, since the recommended indoor levels are A-weighted. The additional Swedish low frequency third octave band requirements might be violated. Therefore, there is a need for revi

Computer usage with cold hands; an experiment with pointing devices.

Computers are used in the outdoors and in connection with cold store work. Cold hand and fingers limit data input, as studied here. Six input devices; trackballs, pens, and a mouse were tested by 19 participants in a Fitts' target acquisition task with 2 target sizes under 2 experimental conditions; warm and cold right hand. Measures were acquisition times, number of errors, participant's preferen