Effect of Heat Treatment on Bovine β Lactoglobulin A, B and C. Explored Using Thiol Availability and Fluorescence.
Dilute solutions of -lactoglobulin (-Lg) A, B, and C were heated at temperatures between about 40 and 94 C for 10 min, cooled, and analyzed using Trp fluorescence and extrinsic fluorescence spectra of the probe 1,8-anilinonaphthalene sulfonate (ANS). Thiol availabilities using 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) were determined using a separate set of samples. The normalized ANS fluorescenc