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Site-specific photocoupling of pBpa mutated scFv antibodies for use in affinity proteomics

Recombinant antibody libraries can provide a source of renewable and high-performing binders tailored for use in affinity proteomics. In this context, the process of generating site-specific 1:1 tagging/functionalization and/or orientated surface immobilization of antibodies has, however, proved to be challenging. Hence, novel ways of generating such engineered antibodies for use in affinity prote

Visual Odometry from Two Point Correspondences and Initial Automatic Tilt Calibration

Ego-motion estimation is an important step towards fully autonomous mobile robots. In this paper we propose the use of an initial but automatic camera tilt calibration, which transforms the subsequent motion estimation to a 2D rigid body motion problem. This transformed problem is solved 2-optimally using RANSAC and a two-point method for rigid body motion. The method is experimentally evaluated u

Kan ett ökat biobränsleuttag kombineras med god miljöhänsyn? : Resultat av en intervjustudie med skogsbrukets aktörer

Det är många maskiner och människor engagerade i en avverkning där man tar ut virke, grot och stubbar. En serie djupintervjuer med olika intressenter och aktörer visar att det finns stora brister i planering, logistik och kommunikation mellan de olika leden i kedjan, speciellt då flera olika entreprenörer är inblandade. Svaren visar också att det finns ett behov av bättre utbildning – och att man

District heating and cooling systems - Framework for Modelica-based simulation and dynamic optimization

Future district heating systems (so called 4th Generation District Heating (4GDH) systems) have to address challenges such as integration of (de)centralized renewable energy sources and storage, low system temperatures and high fluctuation of the supply temperature. This paper presents a novel framework for representing and simplifying on-grid energy systems as well as for dynamic thermo-hydraulic

Cysteinyl leukotriene receptor 1 facilitates tumorigenesis in a mouse model of colitis-associated colon cancer

Cysteinyl leukotriene receptor 1 (CysLT1R) has been shown to be up-regulated in the adenocarcinomas of colorectal cancer patients, which is associated with a poor prognosis. In a spontaneous model of colon cancer, CysLT1R disruption was associated with a reduced tumor burden in double-mutant female mice (ApcMin/+/Cysltr1-/-) compared to ApcMin/+ littermates. In the current study, we utilized a gen

Integrating engineering design and design analysis activities at an operational level

Computer-based design analysis is nowadays of utmost importance for most engineering design projects. However, this brings some challenges, among them that of the collaboration between engineering designers and design analysts. Since they work with, and are responsible for, different areas, they do not necessarily have full insight into each other’s way of working. The issue of integration between

Modelling of the integration tasks of product and packaging development

PurposeThere is a growing demand for a concurrent development of product and packaging in order to better take into account the supply chain needs. Different tasks are required to integrate packaging and product development: 1) At the strategic level, it is necessary to define or refine an integrated development policy; 2) For each new development project, an adaptation of this policy must be unde