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Nonsense-mediated mRNA decay (NMD) is a conserved eukaryotic quality control mechanism which triggers decay of mRNAs harboring premature translation termination codons. In this chapter, I describe methods for monitoring the influence of NMD on mRNA abundance and decay rates in Saccharomyces cerevisiae. The descriptions include detailed methods for growing yeast cells, total RNA isolation, and Nort
The yeast Saccharomyces cerevisiae provides an ideal model system for elucidation of the molecular mechanisms that regulate the nonsense-mediated mRNA decay (NMD) pathway. This chapter describes an array of molecular biological, genetic, and biochemical tools that facilitate the characterization of transcripts that comprise NMD substrates and provide insights into the roles of the upf/nmd proteins
Trots att urbaniseringen är negativ för biologisk mångfald lyfts urbana miljöer ofta fram som en möjlig tillflyktsort för arter. Dessutom har kunskapen om hur biologisk mångfald bidrar till goda livsmiljöer för människor blivit allt tydligare. I detta nummer av faktabladet kommer vi att utforska hur urbana miljöer kan gynna biologisk mångfald och skapa goda livsmiljöer för vissa arter och människo
This thesis presents fundamental studies on oxide-based catalysts and model catalysts used for the synthesis of renewable fuels. The two investigated oxide catalysts are In2O3(111) model catalysts and NiMo-oxide catalysts.The In2O3(111) surface is studied as a model system for In2O3-based catalysts used for CO2 hydrogenation to methanol. Specifically, the interaction of In2O3(111) with CO2, syngas
The evolution of reading frame maintenance must have been an early event, and presumably preceded the emergence of the three domains Archaea, Bacteria and Eukarya. Features evolved early in reading frame maintenance may still exist in present-day organisms. We show that one such feature may be the modified nucleoside 1-methylguanosine (m(1)G37), which prevents frameshifting and is present adjacent
Varje år dör cirka 90 000 personer i Sverige, en klar majoritet av dessaberör på olika sätt människobehandlande professioner. Att stå infördöden eller vara anhörig till någon som har dött eller ska dö måste räknas till några av de situationer där en människa kan ses som mest utsatt. Vad finns det då för ambitioner om vilka kunskaper och kompetens de som möter dessa människor ska besitta? Att möta
Situated in the intersection of the literature on QoL and lifestyle migration, this paper contributes to the discussion of three subjective components of QoL after migrating to another country. These are hedonic (happiness), evaluative (life satisfaction) and eudaimonic (having a purposeful or worthwhile life). Based on 36 in-depth interviews to Swedish citizens permanently living in Portugal, a g
After a tank has been exposed to crash violence, or an external fire, it might, in some situations, be judged dangerous to move the vessel due to the risk of a sudden tank rupture. Therefore, Swedish rescue services have a long history of using rifles to penetrate and therefore depressurize the vessels. In this paper, some first steps on providing guidance on the selection of ammunition and requirAfter a tank has been exposed to crash violence, or an external fire, it might, in some situations, be judged dangerous to move the vessel due to the risk of a sudden tank rupture. Therefore, Swedish rescue services have a long history of using rifles to penetrate and therefore depressurize the vessels. In this paper, some first steps on providing guidance on the selection of ammunition and requir
History shows that wars can be enormously disruptive of economic activity, especially international trade, national income, and global economic welfare. This article analyzes the impact on the European regions the Russian invasion of Ukraine using the largest and most updated multiregional input-output model for Europe. Two shocks, trade and inflation, and four alternative scenarios are simulated.
This paper discusses the growing regional socio-economic disparity in Sweden and investigates two regions’ power relationship with the economically and politically dominant Stockholm region: one region with a negative economic trajectory and the other with a positive economic development over 25 years. The conceptual framework is based on the topography/topology nexus, using the key concepts of ‘r
This paper discusses how housing for refugees is delt with by the authorities in rural Finland and Sweden. More precisely, we look at the services systems, rights and obligations which guide the choices of immigrants once they are allowed so stay, at least temporarily, in respective country. We apply the ‘conflicted policy processes’ approach in intergovernmental relations and Peter Scholten’s gov
Based on disappointing results of stem cell-based application in clinical trials for patients with critical limb ischemia, we hypothesized that the acidic environment might be the key factor limiting cell survival and function. In the present study, we used microdialysis to determine presence of acidosis and metabolic imbalance in critical ischemia. Moreover, we explored the effect of extracellula
