Analysis of vitamin D and its metabolites in biological samples – Part I : Optimization and comparison of UHPSFC-MS/MS and UHPLC-MS/MS methods
Fat-soluble vitamin D is an essential bioactive compound important for human health. Insufficient vitamin D levels can result not only in bone disease but also in other disorders, such as cancer, metabolic disorders, and diseases related to poor immune function. The current methods commonly used for vitamin D analysis are often applied to determine the levels of the most abundant metabolite in pla