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Extended analysis of the TiI spectrum: 3d-4f, 4d-4f and 4f-5g transitions

Titanium spectra recorded in the infrared wavelength region with a Fourier Transform Spectrometer have been analysed, and 572 lines have been identified as transitions of the types 3d-4f, 4d-4f and 4-5g. 11 lines in the UV region around 2400 angstrom have been identified as belonging to the 3d(3)4s a(5)F-3d(2)4s(F-4)4f transition array. 113 levels of the 3d(2)4s(F-4)4f, 3d(3)(F-4)4f and 3d(2)4s(F-

Modulation of inflammatory mediators and ppargammaand nfkappab expression by pravastatin in response to lipoproteins in human monocytes in vitro.

Statins are inhibitors of the rate-limiting step of cellular cholesterol synthesis. In vitro and in vivo studies suggest that statins have anti-inflammatory properties independent of their cholesterol-lowering effects. These observations prompted us to examine the effects of pravastatin (50 mgr; M) and native or oxidized low density lipoprotein (nLDL or oxLDL) (50 mgr; g ml(minus sign1)) on primar

Oncoretroviral gene transfer to NOD/SCID repopulating cells using three different viral envelopes

Background The aim of this study was to investigate gene transfer to human umbilical cord blood (CB) CD34(+)/CD38(low) and NOD/SCID repopulating cells using oncoretroviral vectors and to compare the transduction efficiency using three different viral envelopes. Methods CB cells were transduced on Retronectin using an MSCV-based vector with the gene for GFP (MGIN), which was packaged into three dif

Adenovirus-mediated overexpression of murine cyclic nucleotide phosphodiesterase 3B

To construct the recombinant adenovirus vector containing the cDNA for recombinant mouse cyclic nucleotide phosphodiesterase 3B (mPDE3B), the cDNA for mPDE3B was subcloned into pACCMV.pLpA. Subsequently, this recombinant plasmid, pACCMV.mPDE3B, was cotransfected with pJM17 plasmid containing the adenoviral genome into 293 human embryonic kidney cells, and the replication-deficient adenovirus AdCMV

Extracytoplasmatic processes impaired by inactivation of the trxA (thioredoxin gene) in Bacillus subtilis

The trxA gene is regarded as essential in Bacillus subtilis, but the roles of the TrxA protein in this gram-positive bacterium are largely unknown. Inactivation of trxA results in deoxyribonucleoside and cysteine or methionine auxotrophy. This phenotype is expected if the TrxA protein is important for the activity of the class Ib ribonucleotide reductase and adenosine-5'-phosphosulfate/3'-phosphoa

Challenges and opportunities for medications development in alcoholism: An international perspective on collaborations between academia and industry

This article represents the proceedings of a symposium presented at the 12th Congress of the International Society for Biomedical Research on Alcoholism held in Heidelberg/Mannheim, Germany, on September 30, 2004. The organizers and cochairs were Bankole A. Johnson, DSc, MD, PhD, and Karl Mann, MD. The presentations included the following: (1) A Perspective from Academia, by Bankole A. Johnson, DS

On the theory of low-density convolutional codes

We introduce and analyze a new statistical ensemble of lowdensity parity-check convolutional (LDC) codes. The result of the analysis are bounds, such as a lower bound for the free distance and upper bounds for the burst error probability of the LDC codes.

Pion and kaon electromagnetic form factors

We study the electromagnetic form factor of the pion and kaons at low-energies with the use of Chiral Perturbation Theory. The analysis is performed within the three flavour framework and at next-to-next-to-leading order. We explain carefully all the relevant consistency checks on the expressions, present full analytical results for the pion form factor and describe all the assumptions in the anal

Synthesis and reactivity studies of a manganese microperoxidase containing b-type heme

Mn(III) protoporphyrin IX-6(7)-gly-gly-his methyl ester (MnGGH) has been prepared by condensation of glycyl-glycyl-L-histidine methyl ester with the propionic side chains of Mn(III) protoporphyrin IX. It was characterised by mass spectrometry and UV/VIS spectroscopy. Stopped-flow spectrophotometry was used to study the reaction of the Mn microperoxidase with hydrogen peroxide. The formation of act

Volumetric intensity dependence on the formation of molecular and atomic ions within a high intensity laser focus

The mechanism of atomic and molecular ionization in intense, ultra-short laser fields is a subject which continues to receive considerable attention. An inherent difficulty with techniques involving the tight focus of a laser beam is the continuous distribution of intensities contained within the focus, which can vary over several orders of magnitude. The present study adopts time of flight mass s

Contamination of pasteurised milk by Bacillus cereus in the filling machine

The contamination of pasteurized milk by Bacillus cereus during the ®lling process was studied in two dairy plants. Samples of pasteurized milk were taken at four different sites along the production line. The samples were stored at 7 °C for 7 d, or at 10 °C for 5 d, before plate counting and random selection of B. cereus isolates. Isolates of B. cereus were typed by the polymerase chain reaction

Polymorphisms in telomere-associated genes, breast cancer susceptibility and prognosis

Telomeres are essential structures for maintaining chromosomal stability and their length has been reported to correlate with cancer risk and clinical outcome. Single nucleotide polymorphisms (SNPs) in genes encoding telomere-associated proteins could affect telomere length and chromosomal stability by influencing gene expression or protein configuration in the telomeres. Here, we report the resul

Chance or necessity? Insertional mutagenesis in gene therapy and its consequences

Recently, unusual forms of leukemias have developed as complications following retroviral transfer of potentially therapeutic genes into hematopoietic cells. A crucial component in the pathogenesis of these complications was the upregulation of a cellular proto-oncogene by random insertion of the retroviral gene transfer vector. These findings have great implications for the genetic manipulation o