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The N-terminal N5 subdomain of the alpha 3(VI) chain is important for collagen VI microfibril formation

Collagen VI assembly is unique within the collagen superfamily in that the alpha 1(VI), alpha 2(VI), and alpha 3(VI) chains associate intracellularly to form triple helical monomers, and then dimers and tetramers, which are secreted from the cell. Secreted tetramers associate end-to-end to form the distinctive extracellular microfibrils that are found in virtually all connective tissues. Although

Reduced grazing rates in Daphnia pulex caused by contaminants: Implications for trophic cascades

Ecotoxicological endpoints based on behavioral traits (e.g., predator avoidance, feeding, and locomotion) may be more sensitive and give more insights into patterns of sublethal toxicity than survivorship tests. In this study, the density-dependent grazing rate of Daphnia pulex pre-exposed to p,p'-dichlorodiphenyldichloroethylene (DDE) (insecticide metabolite) and glyphosate (herbicide), via water

Applications of two-photon absorption for detection of CO in combustion gases

Laser-induced fluorescence has been used for detection of CO in different environments. The fluorescence light was obtained by using a two-photon transition between theX1?- and theB1?- electronical states around 230 nm. Cell measurements indicate a detection limit lower than 0.1 ppm. Measurements in a CH4/air flame and in a low pressure dc discharge were realized with a diode-array detector, which

Alteration of intestinal microflora is associated with reduction in abdominal bloating and pain in patients with irritable bowel syndrome

OBJECTIVE: The influence of the gastrointestinal (GI) microflora in patients with irritable bowel syndrome (IBS) has not been clearly elucidated. This study was undertaken to see if patients with IBS have an imbalance in their normal colonic flora, as some bacterial taxa are more prone to gas production than others. We also wanted to study whether the flora could be altered by exogenous supplement

Predicting the Displacement of Yogurt by Water in a Pipe Using CFD

A numerical scheme based on the volume of fluid (VOF) method for predicting the displacement of one liquid by another has been verified versus electrical resistance tomography (ERT) and ultrasonic velocity profile (UVP) measurements for the displacement of yoghurt by water. The scheme using the VOF method predicts the skewed phase distribution as measured using ERT and the global structure of the

International Biofuel Trade - A Study of the Swedish Import

Following the development of large-scale use of biomass energy in the EU, international biofuel trade is a plausible scenario and something that is already taking place in Northern Europe. This paper focuses on Swedish biofuel imports, both direct and indirect imports, the latter which derive from the fact that part of the imported pulpwood and timber end up as fuel. The objective is to describe t

Transport properties and aggregation phenomena of polyoxyethylene sorbitane monooleate (polysorbate 80) in pig gastrointestinal mucin and mucus

The aqueous environment in the gastrointestinal tract frequently requires solubilization of hydrophobic drug molecules in appropriate drug delivery vehicles. An effective uptake/absorption and systemic exposure of a drug molecule entails many processes, one being transport properties of the vehicles through the mucus layer. The mucus layer is a complex mixture of biological molecules. Among them,

Precise in situ thickness analysis of epitaxial graphene layers on SiC(0001) using low-energy electron diffraction and angle resolved ultraviolet photoelectron spectroscopy

We demonstrate an easy and practical method for the thickness analysis of epitaxial graphene on SiC(0001) that can be applied continuously during the preparation procedure. Fingerprints in the spot intensity spectra in low energy electron diffraction (LEED) allow for the exact determination of the number of layers for the first three graphene layers. The LEED data have been correlated with the ele

Identification, synthesis and activity of sex pheromone gland components of the autumn gum moth (Lepidoptera : Geometridae), a defoliator of Eucalyptus

The autumn gum moth, Mnesampela privata (Guenee) (Lepidoptera: Geometridae), is native to Australia and can be a pest of plantation eucalypts. Field-collected and laboratory-reared female autumn gum moths were dissected to remove glands likely to contain components of the sex pheromone. Using gas chromatography (GC) and combined gas chromatography-mass spectrometry (GC-MS), three compounds were id

Experimental observation of different-order components of a vibrational wave packet in a bulk dielectric using high-order Raman scattering

We use high-order Raman scattering in a bulk dielectric to characterize coherent dynamics with precision typical for gas phase experiments. The experimental pump-probe approach allows for the simultaneous observation and separation in space and time of the individual contributions of different-order Raman processes to a coherent wave packet without relying on phase-matching conditions and within t

Genetic profiling of colorectal cancer liver metastases by combined comparative genomic hybridization and G-banding analysis

The majority of genetic studies of colorectal carcinogenesis have focused on changes found in primary tumors. Despite the fact that liver metastases are a leading cause of colorectal cancer deaths, the molecular genetic basis of the advanced disease stages remains poorly understood. We performed comparative genomic hybridization (CGH) on 17 liver metastases from colorectal carcinomas and compared

Effect of matrix elasticity on affinity binding and release of bioparticles. Elution of bound cells by temperature-induced shrinkage of the smart macroporous hydrogel

The first step of bacterial or viral invasion is affinity and presumably multisite binding of bioparticles to an elastic matrix like a living tissue. We have demonstrated that model bioparticles such as inclusion bodies (spheres of about 1 mu m in size) Escherichia coli cells (rods 1 x 3 mu m), yeast cells (8 mu m spheres), and synthetic microgel particles (0.4 mu m spheres) are binding via differ

Evaluation of seven different enzyme-linked immunosorbent assays for serodiagnosis of Staphylococcus aureus bacteremia

Objective: Serologic assays for Staphylococcus aureus antibodies were evaluated regarding their ability to differentiate between uncomplicated and complicated S. aureus bacteremia, between S. aureus and non-S. aureus bacteremia, and between S. aureus and non-S. aureus endocarditis. Methods: Enzyme-linked immunosorbent assays (ELISAs) were performed to measure Ig G antibodies against seven S. altre

Cloud condensation nuclei in pristine tropical rainforest air of Amazonia: size-resolved measurements and modeling of atmospheric aerosol composition and CCN activity

Atmospheric aerosol particles serving as cloud condensation nuclei (CCN) are key elements of the hydrological cycle and climate. We have measured and characterized CCN at water vapor supersaturations in the range of S=0.10-0.82% in pristine tropical rainforest air during the AMAZE-08 campaign in central Amazonia. The effective hygroscopicity parameters describing the influence of chemical composit