Trimethylselenonium ion determination in human urine by high-performance liquid chromatography–hydride generation–atomic fluorescence spectrometry optimization of the hydride generation step
This work describes the intricacies of the determination of the trimethylselenonium ion (TMSe) in human urine via high-performance liquid chromatography–hydride generation–atomic fluorescence spectrometry (HPLC-HG-AFS). By definition, this technique requires that the separated TMSe can be online converted into a volatile compound. Literature data for the determination of TMSe via the hydride gener