Bi-enzyme biosensor based on NAD(+)- and glutathione-dependent recombinant formaldehyde dehydrogenase and diaphorase for formaldehyde assay
The present work reports on the development of a bi-enzyme biosensor using diaphorase from Bacillus stearothermophilus, nicotinamide adenine dinucleotide (NAD(+))- and glutathione (GSH)-dependent formaldehyde dehydrogenase (FDH) from the genetically-engineered methylotrophic yeast Hansenula polymorpha as bio-recognition elements. The sensor architecture comprises a first layer containing diaphoras
