Hydroxyapatite as a novel reversible in situ adsorption matrix for enzyme thermistor-based FIA
The application of the easily available and inexpensive chromatographic matrix hydroxyapatite for reusable and reversible immobilization of enzymes for enzyme thermistor-based flow injection analysis of glucose and Urea was tested. The immobilization was achieved by simple affinity adsorption of glucose oxidase and urease by a suitable pH-induced alteration of the protein charge. A linear detectio
