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The Na(+) transport in Gram-positive bacteria defect in the Mrp antiporter complex measured with (23)Na-NMR.

(23)Na-NMR has previously been used to monitor Na(+) translocation across membranes in Gram-negative bacteria and in various other organelles and liposomes using a membrane-impermeable shift reagent to resolve the signals resulting from internal and external Na(+). In this work, the (23)Na-NMR method was adapted for measurements of internal Na(+) concentration in the Gram-positive bacterium Bacill

Line-of-Sight Obstruction Analysis for Vehicle-to-Vehicle Network Simulations in a Two Lane Highway Scenario

In vehicular ad-hoc networks (VANETs) the impact of vehicles as obstacles has largely been neglected in the past. Recent studies have reported that the vehicles that obstruct the line-of-sight (LOS) path may introduce 10-20 dB additional loss, and as a result reduce the communication range. Most of the traffic mobility models (TMMs) today do not treat other vehicles as obstacles and thus can not m

Dependence on Vitamin K-dependent Protein S for Eukaryotic Cell Secretion of the beta-Chain of C4b-binding Protein

The anticoagulant vitamin K-dependent protein S (PS) circulates in plasma in two forms, 30% free and 70% being bound to the complement regulatory protein C4b-binding protein (C4BP). The major C4BP isoform consists of 7 alpha-chains and 1 beta-chain (C4BP beta(+)), the chains being linked by disulfide bridges. PS binds to the beta-chain with high affinity. In plasma, PS is in molar excess over C4BP

Longitudinal changes in weight in relation to smoking cessation in participants of the EPIC-PANACEA study

Purpose. We assessed the association between smoking cessation and prospective weight change in the European population of the European Prospective Investigation into Cancer and Nutrition-Physical Activity, Nutrition, Alcohol, Cessation of smoking. Eating out of home And obesity (EPIC-PANACEA) project. Methods. The study involved more than 300,000 healthy volunteers, recruited between 1992 and 200

Economic Development and Prosperity Patterns Around the World: Structural Challenges for a Global Steady-State Economy

Taking a global perspective this paper sets out to theoretically and empirically identify prosperity patterns for four groups of countries at different levels of economic development. It conceptualizes ‘prosperity’ in terms of ecological sustainability, social inclusion, and the quality of life and contextualizes this definition in global perspective. Subsequently, it operationalizes and measures

Understanding risk in forest ecosystem services: implications for effective risk management, communication and planning

Uncertainty, insufficient information or information of poor quality, limited cognitive capacity and time, along with value conflicts and ethical considerations, are all aspects that make risk management and risk communication difficult. This paper provides a review of different risk concepts and describes how these influence risk management, communication, and planning in relation to forest ecosy

A characterization of $(-1,-1)$-Freudenthal-Kantor triple systems

In this paper, we discuss a connection between $(−1,−1)$-Freudenthal–Kantor triple systems, anti-structurable algebras, quasi anti-flexible algebras and give examples of such structures. The paper provides the correspondence and characterization of a bilinear product corresponding a triple product.

Tomato thymidine kinase-based suicide gene therapy for malignant glioma-an alternative for Herpes Simplex virus-1 thymidine kinase.

Malignant gliomas (MGs) are the most common malignant primary brain tumors with a short life estimate accompanied by a marked reduction in the quality of life. Herpes Simplex virus-1 thymidine kinase ganciclovir (HSV-TK/GCV) system is the best characterized enzyme prodrug therapy in use. However, lipophobicity of GCV and low enzymatic activity of HSV-TK reduce the treatment efficacy. Tomato TK (To

A dinuclear zinc(II) complex of a new unsymmetric ligand with an N5O2 donor set; A structural and functional model for the active site of zinc phosphoesterases.

The dinuclear complex [Zn2(DPCPMP)(pivalate)](ClO4), where DPCPMP is the new unsymmetrical ligand [2-(N-(3-((bis((pyridin-2-yl)methyl)amino)methyl)-2-hydroxy-5-methylbenzyl)-N-((pyridin-2-yl)methyl)amino)acetic acid], has been synthesized and characterized. The complex is a functional model for zinc phosphoesterases with dinuclear active sites. The hydrolytic efficacy of the complex has been inves

Developing an understanding of social norms and games : Emotional engagement, nonverbal agreement, and conversation

The first part of the article examines some recent studies on the early development of social norms that examine young children’s understanding of codified rule games. It is argued that the constitutive rules than define the games cannot be identified with social norms and therefore the studies provide limited evidence about socio-normative development. The second part reviews data on children’s p

Improved tool wear properties in sheet metal forming using Carbide steel, a novel abrasion resistant cast material

Abstract in UndeterminedThis work presents a comparative study regarding wear behavior between a novel abrasion resistant cast iron, "Carbide Steel" and a number of conventional tool materials frequently used in the automotive industry. Carbide Steel is basically a high-Cr white cast iron heavily alloyed with cemented carbide. The high chromium content and additional alloying elements like WC. TiC

An International Relations perspective on the global politics of carbon dioxide capture and storage

With the publication of the IPCC Special Report on Carbon dioxide Capture and Storage (CCS), CCS has emerged as a focal issue in international climate diplomacy and energy collaboration. This paper has two goals. The first goal is to map CCS activities in and among various types of intergovernmental organisations; the second goal is to apply International Relations (IR) theories to explain the gro

Management of acute stroke in patients taking novel oral anticoagulants

Each year, 1 center dot 0-2 center dot 0% of individuals with atrial fibrillation and 0 center dot 1-0 center dot 2% of those with venous thromboembolism who are receiving one of the novel oral anticoagulants (dabigatran, rivaroxaban, or apixaban) can be expected to experience an acute ischemic stroke. Additionally, 0 center dot 2-0 center dot 5% of individuals with atrial fibrillation who are rec

High Prevalence of Atrial Fibrillation Among Patients With Ischemic Stroke.

Atrial fibrillation (AF) is a common cause of devastating but potentially preventable stroke. Estimates of the prevalence of AF among patients with stroke vary considerably because of difficulties in detection of intermittent, silent AF. Better recognition of AF in this patient group may help to identify and offer protection to individuals at risk. Our aim was to determine the nationwide prevalenc

Heparin-binding protein: An early indicator of critical illness and predictor of outcome in cardiac arrest.

AIM: To investigate plasma levels of the neutrophil-borne heparin-binding protein (HBP) in patients with induced hypothermia after cardiac arrest (CA), and to study any association to severity of organ failure, incidence of infection and neurological outcome. METHODS: This study included 84 patients with CA of mixed origin who were treated with hypothermia. Plasma samples from 7 time points during

Exploring single-photon ionization on the attosecond time scale

One of the fundamental processes in nature is the photoelectric effect in which an electron is ripped away from its atom via the interaction with a photon. This process was long believed to be instantaneous but with the development of attosecond pulses (1 as 10(-18) s) we can finally get an insight into its dynamic. Here we measure a delay in ionization time between two differently bound electrons

Generation of miniaturized planar ecombinant antibody arrays using a microcantilever-based printer.

Miniaturized (Ø 10 μm), multiplexed (>5-plex), and high-density (>100 000 spots cm(-2)) antibody arrays will play a key role in generating protein expression profiles in health and disease. However, producing such antibody arrays is challenging, and it is the type and range of available spotters which set the stage. This pilot study explored the use of a novel microspotting tool, Bioplume(TM)-cons