Simultaneous saccharification and fermentation of steam-pretreated willow
Ethanol was produced from SO2-impregnated steam-pretreated willow by simultaneous saccharification and fermentation (SSF). Experiments with SSF were performed in stirred 300-ml vessels and in a 3-1 fermenter. Commercial cellulases were used in combination with either Saccharomyces cerevisiae or Zymomonas mobilis. The amounts of substrate, enzymes, yeast, bacteria, and nutrients were varied. The te
