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Evaluation of quantitative thin layer chromatography using staining reagents

Thin layer chromatography (TLC) using staining reagents is a superior method for analyzing organic compounds without chromophores. It is fast, versatile and sometimes the only viable method. We have investigated quantitative TLC using staining reagents, in combination with modem image analysis software. Our results show that it is possible to get reliable measurements, suitable for high-throughput

Intact and permeabilized cells of the yeast Hansenula polymorpha as bioselective elements for amperometric assay of formaldehyde

Intact and permeabilized yeast cells were tested as the biorecognition elements for amperometric assay of formaldehyde (FA). For this aim, the mutant G 105 (gcr1 catX) of the methylotrophic yeast Hansenula polymorpha with a high activity of AOX was chosen. Different approaches were used for monitoring FA-dependent cell response including analysis of their oxygen consumption rate by the use of a Cl

Mandibular-facial reconstruction with segmentally split serratus anterior composite flaps

Oncologic or traumatic head and neck defects with missing mandible, facial skin, and oral mucosa are especially well suited to reconstruction with a composite tissue unit, based on the subscapular-thoracodorsal vessels, that carries any combination of skin, bone, and muscle to restore vascularized skeletal structures, oral lining, and skin cover. The subscapular-thoracodorsal vascular pedicles sup

Lymphoma and other malignancies in primary sjogren's syndrome A cohort study on cancer incidence and lymphoma predictors.

Objectives: To assess the risk of lymphoproliferative disease or other malignancy (standardised incidence ratios (SIRs)), in patients with primary Sjogren's syndrome according to the American-European Consensus Criteria (AECC), compared with patients with sicca syndrome (non-AECC) and the background population. To identify predictors of malignancy and describe lymphoma types and survival probabili

Intermittent and daily smokers: two different socioeconomic patterns, and diverging influence of social participation

OBJECTIVE: To investigate socioeconomic differences in intermittent and daily smoking, and to assess the association between social participation and these two smoking behaviours.DESIGN/SETTING/PARTICIPANTS/MEASUREMENTS: A population of 11 837 individuals interviewed in 1992-94, aged 45-64 years, was investigated in this cross sectional study. A multivariate logistic regression model was used to a

Comprehensive bioinformatic analysis of the specificity of human immunodeficiency virus type 1 protease

Rapidly developing viral resistance to licensed human immunodeficiency virus type 1 (HIV-1) protease inhibitors is an increasing problem in the treatment of HIV-infected individuals and AIDS patients. A rational design of more effective protease inhibitors and discovery of potential biological substrates for the HIV-1 protease require accurate models for protease cleavage specificity. In this stud

Wild Landscapes : The Cultural Nature of Swedish National Parks

Since their emergence in the late 19th century, national park spaces have been perceived as articulations of untouched nature or wild landscape beyond society. Yet no understanding of national parks can do without the recognition that they exist in historical spaces created, institutionalised and prepared for them by cultural practices and modes of representation. Rather than being given by nature

On Degradation and Monitoring Tools for Gas and Steam Turbines

Popular Abstract in Swedish Elmarknadens avreglering har medfört att behovet av "condition monitoring" eller tillståndskontroll har ökat. Den nya "marknaden" har medfört en annan typ av aktörer, som till skillnad mot de traditionella kraftbolagen, inte har samma kultur beträffande teknikorganisation. Därför har behovet av övervakningssystem ökat markant. Avhandlingen behandlar dessa ur ett teknisThe revenue from a power plant is strongly dependent on the life cycle cost. Today, when the market conditions for power-producing companies have shifted from a protected market to a deregulated market, the need for tools to monitor power plants has increased significantly. In this new competitive market, targeted revenues and operational economy drive the need for advanced monitoring tools. In th

EXAFS measurements of metal-decorated nanocavities in Si

This contribution presents a sample preparation methodology to enable the identification, with synchrotron radiation-based analytical techniques, of the gettering sites of metallic impurities on the internal walls of implantation-induced nanocavities in Si substrates. Preliminary results for the Cu-Si and Cu-Cu bond lengths on the internal surface of the nanocavities are reported. (C) 2002 Elsevie

Influence of pH on the adsorptive fouling of ultrafiltration membranes by fatty acid

Fatty acids are found in many solutions treated in ultrafiltration plants, for example, in dairy products, fermentation broth, oily waste water and bleach plant effluents from pulp mills. The influence of fatty acids on the fouling of membranes is often ignored because their concentration is rather low. However, during recent years the significant influence of fatty acids on the flux reduction of

Towards industrial pentose-fermenting yeast strains

Production of bioethanol from forest and agricultural products requires a fermenting organism that converts all types of sugars in the raw material to ethanol in high yield and with a high rate. This review summarizes recent research aiming at developing industrial strains of Saccharomyces cerevisiae with the ability to ferment all lignocellulose-derived sugars. The properties required from the in

Treatment with anti-factor VIIa in acute pancreatitis in rats: Blocking both coagulation and inflammation?

Objective. Acute pancreatitis starts as an autodigestive process restricted to the pancreas and progresses to a systemic inflammation via cytokine release into the blood stream. Several inhibitors of the coagulation cascade, including active- siteinactivated factor VIIa, have shown anti- inflammatory properties in other inflammatory models than acute pancreatitis. Free radical scavengers have prov

Integrated Test Scheduling, Test Parallelization and TAM Design

We propose a technique integrating test scheduling, scan chain partitioning and test access mechanism (TAM) design minimizing the test time and the TAM routing cost while considering test conflicts and power constraints. Main features of our technique are (1) the flexibility in modelling the systems test behaviour and (2) the support for interconnection test of unwrapped cores and user-defined log