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Assessment of the colon innervation with serial biopsies above the aganglionic zone before the pull-through procedure in Hirschsprung's disease

Different types of colonic dysganglionosis, and in particular intestinal neuronal dysplasia (IND) have been blamed for certain postoperative complications after surgery for Hirschsprung's disease (HD). We prospectively assessed colon innervation above the aganglionic zone (AZ) before proceeding to pull-through (PT) in order to rule-out IND as a cause of those complications. We first used a two-sta

Gold-coated capillary based 2,4-dichlorophenoxyacetic acid chemi-lumincscent assays: possibilities towards multianalysis

The application of gold-coated glass capillaries for the design of a sensitive chemiluminescent immunoassay for 2,4-dichlorophenoxyacetic acid (2,4-D) is reported. The gold coating on the glass capillaries has been partially characterized and its effect on enhancing the signal intensity has been measured. A simple photo-multiplier tube-based photon detector is used for this purpose. At least three

On the formula of Jacques-Louis Lions for reproducing kernels of harmonic and other functions

We give a simpler proof of the formula, due to J.-L. Lions, for the reproducing kernel of the space of harmonic functions on a domain Omegasubset ofR(n) whose boundary values belong to the Sobolev space H-s(partial derivativeOmega), and also obtain generalizations of this formula when instead of harmonic functions one considers functions annihilated by a given elliptic partial differential operato

Intramolecular vibronic dynamics in molecular solids: C60

Vibronic coupling in solid C60 has been investigated with a combination of resonant photoemission spectroscopy (RPES) and resonant inelastic x-ray scattering (RIXS). Excitation as a function of energy within the lowest unoccupied molecular orbital resonance yielded strong oscillations in intensity and dispersion in RPES, and a strong inelastic component in RIXS. Reconciling these two observations

Results from a nationwide postmarketing cohort study of patients in Sweden treated with etanercept

Objectives: To describe a nationwide system for postmarketing follow up of new antirheumatic drugs in Sweden, and to analyse safety and effectiveness in an etanercept treated patient cohort. Methods: Etanercept became available in Sweden for prescribing on a named patient basis in 1999. All patients treated were included in a follow up of intensified adverse event reporting and recording of clinic

Redstarts Phoenicurus phoenicurus mimicking the song of the Scarlet Rosefinch Carpodacus erythrinus.

Over a period of three years, at least three different males of Common Redstarts Phoenicurus phoenicurus in southern Sweden mimicked the song of Scarlet Rosefinch Carpodacus erythrinus. At least two of the Redstarts were second calendar year birds that were heard to mimic Rosefinch song in spring long before the arrival of the local breeding population of Scarlet Rosefinch. Since Redstarts and Ros

Self-aggregation of squid cranial cartilage proteoglycans

Squid cranial cartilage has been found to contain three different proteoglycan populations, two of which form aggregates (Vynios, D.H. and Tsiganos, C. P., Biochim. Biophys. Acta 1033: 139-147, 1990). The aggregation involves interaction of their protein cores as assessed by electron microscopy and biochemical data. Aggregating oligopeptides were isolated after mild trypsin digestion which inhibit

Reconstruction Techniques and Finite Volume Schemes for Hyperbolic Conservation Laws

This thesis concerns the numerical approximation of the solutions to hyperbolic conservation laws. In particular the research work focuses on reconstruction techniques; the reconstruction being the key ingredient in modern finite volume schemes aiming to increase spatial order of accuracy. To better conform to the nature of the solutions to the hyperbolic problems, the reconstructing fu

Optic nerve hypoplasia: Risk factors and epidemiology.

OBJECTIVES: To study the epidemiology of optic nerve hypoplasia. DESIGN AND METHODS: Children with optic nerve hypoplasia and visual impairment were identified through the Swedish Register of Visually Impaired Children. Pre- and perinatal characteristics were obtained from the Medical Birth Registry and by scrutinizing pregnancy and delivery records. Clinical characteristics of children with optic

Effect of mixing time on gluten recovered by ultracentrifugation studied by microscopy and rheological measurements

The effect of mixing time on gluten formation was studied for four commercial flour mixtures. The gluten phase was separated from dough using a nondestructive ultracentrifugation method. Small I deformation dynamic theological measurements and light and scanning electron microscopy were used. The recovered gluten was relatively pure with a small amount of starch granules embedded. The protein matr

Glucose dependence of insulinotropic actions of pituitary adenylate cyclase-activating polypeptide in insulin-secreting INS-1 cells.

The cAMP-elevating pituitary adenylate cyclase-activating polypeptide (PACAP) stimulates insulin release in pancreatic B-cells. Here, we have investigated its potentiating action in rat insulinoma INS-1 cells. In intact cells, PACAP-27 (100 nM) stimulated glucose-induced insulin secretion by >60%. Using the patch-clamp technique with single-cell exocytosis monitored as increases in cell capacitanc

SH3-domain binding function of HIV-1 Nef is required for association with a PAK-related kinase

HIV-1 Nef has previously been shown to bind to Src homology-3 (SH3) domains of a subset of Src family tyrosine kinases. In addition, Nef has been reported to coprecipitate with a serine/threonine kinase activity termed NAK (for Nef-associated kinase). The identity of NAK remains uncertain, but it has been suggested to represent a novel member of the p21-activated kinase (PAK) family. We report her