BACKGROUND: Experimental manipulation of transfusion-induced alloimmunization is limited in humans by ethical considerations. Conversely, studies of alloimmunization in animal models may not reflect the human immune system closely enough to be of optimal benefit. The development of an in vivo model of human alloimmunization that is amenable to experimental manipulation is thus desirable. STUDY DES

With platelet activation, there is modulation of platelet surface molecule expression. In flow cytometric analyses of in vivo platelet activation, results are often confounded by activation induced in vitro by the preparative procedures. It is particularly important therefore to prevent or retard platelet activation as soon as possible after withdrawal of the blood sample. Taking blood into parafo

To study the cellular immunology of platelet-induced alloimmunization, a murine transfusion model was developed. BALB/c (H-2(d)) recipient mice ware transfused weekly with 2 x 108 platelets or 103 leukocytes from C57BL/6 (H- 2b) donor mice. Recipient antidonor major histocompatibility complex (MHC) class I alloantibodies could be detected in flow cytometric assays by the fifth platelet transfusion

Chronic obstructive pulmonary disease (COPD) is one of the leading causes of death worldwide and there are currently limited treatment options for these patients. The disease is characterized by a reduction in airflow due to chronic bronchitis, as well as airspace enlargement in the distal lung, resulting in a loss of surface area available for gas exchange. At end-stage disease, oxygen therapy an

Background: Patients experiencing high levels of pain after surgery remains a considerable clinical problem. Often, no consensus about the best analgesic treatment is present. The majority of clinical trials regarding postoperative pain, generally, target the average analgesic efficacy. In terms to step forward, a focus on the individual patients´ pain levels is needed and an identification of the

This paper argues that an economic system of large and small technology-based firms which interact through technology-related acquisitions and spin-offs can be highly conducive to overall innovativeness and growth. Based on Williamson's (1975) suggestion of a 'systems solution by classical specialisation', and the empirical research on Swedish technology-based firms reported by Lindholm (1994), an

The literature on regional development increasingly recognizes that agency is an essential but understudied factor in processes of regional structural change. However, much like the fundamental debates in social sciences about agency and structure, work in this field has often remained theoretical. Investigating the role of agency in regional economic development, systematically and at the micro-l

In this paper we present a computational offloadingstrategy with graceful degradation for executing Model PredictiveControl using the cloud. We show a method which allows forseamless control assistance and design of flexible controllers usingthe edge cloud. We examplify using a cyber-physical-system athigh frequency and illustrate how the system can be improvedwhile keeping the computational cost In this paper we present a computational offloading strategy with graceful degradation for executing Model Predictive Control using the cloud. We show a method which allows for seamless control assistance and design of flexible controllers using the edge cloud. We examplify using a cyber-physical-system at high frequency and illustrate how the system can be improved while keeping the computational

To predict the vibroacoustic performance of complex thin-walled structures, an analysis using a finite element model considering structure–acoustic interaction is often required. The acoustic response of such models can be time-consuming to compute and sensitive to minor design changes. These models can be too computationally intense since fast design optimizations must be performed. Moreover, kno

Osteopontin (OPN) plays a role in inflammation via recruitment of neutrophils and tissue remodeling. In this study, we investigated the distribution of OPN-expressing cells in the airway epithelium of normal lung tissue and that from patients with chronic obstructive pulmonary disease (COPD). OPN was detected on the epithelial cell surface of small airways and in scattered cells within the epithel

SNOW 3G is a stream cipher designed in 2006 by ETSI/SAGE, serving in 3GPP as one of the standard algorithms for data confidentiality and integrity protection. It is also included in the 4G LTE standard. In this paper we derive vectorized linear approximations of the finite state machine in SNOW3G. In particular,we show one 24-bit approximation with a bias around 2−37 and one byte-oriented approxim

The Scandinavian region has been at the forefront of energy and environmental policies for decades. While significant effort has been devoted to the supply side and development of technology markets, our study questions the extent to which policy efforts have effectively and explicitly addressed behavioural factors affecting the adoption and the use of low-carbon energy technologies at the individ

This Mutual Learning Exercise (MLE) on Innovation-related Procurement was conducted under the Horizon 2020 Policy Support Facility run by the European Commission’s Directorate-General for Research and Innovation. The 15 countries that participated were: Austria, Belgium - Brussels Region, Estonia, France, Germany, Greece, Latvia, Lithuania, Netherlands, Norway, Portugal, Slovenia, Spain, Sweden an

In recent years, many Swedish municipalities have taken actions and initiatives to support citizens to more sustainable textile and fashion consumption. This study seeks to, on one hand, describe more advances of such initiatives, what actors in municipalities, affiliated organizations, and collaboration partners that initiated the actions. Another aim is to investigate existing government policie

Adeno-associated virus (AAV) capsid modification enables the generation of recombinant vectors with tailored properties and tropism. Most approaches to date depend on random screening, enrichment, and serendipity. The approach explored here, called BRAVE (barcoded rational AAV vector evolution), enables efficient selection of engineered capsid structures on a large scale using only a single screen

AbstractShiga toxin (Stx) is the main virulence factor of enterohemorrhagic Escherichia coli (EHEC). EHEC strains cause gastrointestinal infection and release Stx that can gain access to the circulation. Patients may develop hemolytic uremic syndrome with extensive kidney damage. In the first paper we investigated if blood cell-derived microvesicles released during EHEC infection contain Stx. Toxi

The effect of insulin secretagogues on the incorporation of [32P]ortho-phosphate into phospholipids was studied in microdissected islets from obese-hyperglycemic mice. Increased 32P-labelling was observed after incubation for 60 min with 10 mM l-leucine, 10 mM l-arginine or 20 mM d-glucose. Most of the label occurred in the phosphatidyl inositol fraction. The effect of l-leucine was additive to th