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Parameters influencing protein extraction for whole broths in detergent based aqueous two-phase systems

The parameters important for an optimisation of cloud point extraction in technical scale were investigated using a genetically engineered fusion protein derived from endoglucanase I expressed in Trichoderma reesei and the nonionic polyoxyethylene Agrimul NRE 1205. The key parameters are temperature, detergent concentration, and additional salts. These parameters are interdependent, thus there is

Regulatory effects of aerosolized budesonide and adrenalectomy on the lung content of endothelin-1 in the rat

This study was designed to investigate the effect of inflammation and glucocorticosteroids (GCS) on the content of endothelin-1-like immunoreactivity (ET-LI) in the rat lung. Following intratracheal instillation of Sephadex beads, which induces a long-lasting inflammation in the lung, there was an increase in the lung content of ET-LI measured by RIA. This increase was abolished by locally adminis

Influence of street characteristics, driver category and car performance on urban driving patterns

Driving patterns (i.e., speed, acceleration and choice of gears) influence exhaust emissions and fuel consumption. The aim here is to obtain a better understanding of the variables that affect driving patterns, by determining the extent they are influenced by street characteristics and/or driver-car categories. A data set of over 14,000 driving patterns registered in actual traffic is used. The re

Biological treatment of industrial wastes in a photobioreactor

An algal-bacterial consortium was tested for the treatment from a coke factory. A Chlorella vulgaris strain and a phenol-degrading Alcaligenes sp. were first isolated from the wastewater treatment plant to serve as inocula in the subsequent biodegradation tests. Batch tests were then conducted with samples from the real wastewater or using a synthetic wastewater containing 325 mg phenol/l and 500

Endovascular repair of descending thoracic aortic aneurysms: an early experience with intermediate-term follow-up

PURPOSE: The purpose of this study was to report an initial experience with the endovascular repair of descending thoracic aortic aneurysm. Complications and intermediate-term morphologic changes were identified with the intent of altering patient selection and device design. METHODS: Endografts were placed into 25 patients at high-risk for conventional surgical repair over a 3(1/2)-year period. D

Indicators of bone formation in weight lifters

Physical activity has been suggested to be one of the determinants of bone turnover and to prevent age-related bone loss. To examine this we measured the serum levels of osteocalcin (bone Gla-protein, BGP), C-terminal procollagen peptide (PICP), serum alkaline phosphatase, bone-specific alkaline phosphatase, and S-calcium as indices of bone formation in 19 actively performing and 15 ex-lifters. Al

Moisture permeability of mature concrete and cement paste

Moisture permeabilities (δv) of mature concrete and cement paste with water-cement ratio (w0/C) 0.4, 0.5 and 0.6 have been determined as functions of the relative humidity (RH). These δv can be used to calculate δv of the aggregates including the interfacial transition zone (ITZ). δv of granite has been preliminarily determined as a function of RH.

High-resolution electron spectroscopy of different adsorption states of ethylene on Pd(111)

The adsorption of ethylene at 100 K on clean and oxygen precovered Pd(1 1 1) surfaces and the thermal evolution of the ethylene adsorbate layers have been investigated experimentally by high-resolution electron energy loss spectroscopy (HREELS), high-resolution X-ray photoelectron spectroscopy with synchrotron radiation, thermal desorption spectroscopy and theoretically by ab initio density functi

Differential exoprotease activities confer tumor-specific serum peptidome patterns

Recent studies have established distinctive serum polypeptide patterns through mass spectrometry (MS) that reportedly correlate with clinically relevant outcomes. Wider acceptance of these signatures as valid biomarkers for disease may follow sequence characterization of the components and elucidation of the mechanisms by which they are generated. Using a highly optimized peptide extraction and ma

Temperament and character in adults with Asperger syndrome

To study the personality characteristics of adults with Asperger syndrome, and investigate the value of self-rating personality inventories, we administered the Temperament and Character Inventory (TCI) to 31 outpatients with Asperger syndrome.The TCI is a self-rating personality inventory that has been validated in the Swedish general population. The results were compared with age- and sex-matche

Breeding synchrony does not affect extra-pair paternity in great reed warblers

Breeding synchrony is hypothesised to influence the occurrence and frequency of extra-pair fertilisations (EPFs) in birds irrespective of the social mating system. The two proposed hypotheses make opposite predictions. (1) Synchronous breeding leads to a lower frequency of EPFs because males face a trade-off between mate guarding and obtaining additional matings via extra-pair copulations (EPCs) (

Exciton fine structure splitting in InP quantum dots in GaInP

We have investigated the electronic structure of excitons in InP quantum dots in GaInP. The exciton is theoretically expected to have four states. Two of the states are allowed to optically decay to the ground ( vacuum) state in the dipole approximation. We see these two lines in photoluminescence (PL) experiments and find that the splitting between the lines ( the fine structure splitting) is 150

Strategies for Reduced Energy Use and Cost in Pulp and Paper Mills by Energy Integration of the Paper Drying Process

The cost of energy for drying paper is substantial for the pulp and paper industry, and studies of various strategies for energy cost reductions such as increased energy efficiency, increased heat recovery from the exhaust drying air and reduced cost of the process heat are presented in this thesis. The energy use and cost studies were performed using a block-model based simulation tool developed