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Genetic engineering has been used to construct hydrophobically modified fusion proteins of cutinase from Fusarium solani pisi and tryptophan-containing peptides. The aim was to enhance the partitioning of the tagged protein in a novel aqueous two-phase system formed by only one water-soluble polymer. The system was based on a hydrophobically modified random copolymer of ethylene oxide (EO) and pro

By using an experimental setup yielding the acoustical Two-Port properties of earplugs. the attenuation of three fundamentally different types of earplugs is predicted. The setup used is based on molds of the ear canals of a real person, and the Two-Port of each earplug is numerically coupled to an acoustical model of the ear. The predicted attenuation is compared to the subjective attenuation (RE

We investigate spin-dependent transport properties of a two-terminal electron waveguide, attached with a side arm, with Rashba spin-orbit interaction (SOI). The interference between the bound states in the side arm and the continuous states in the straight channel gives rise to broad valleys in the conductance spectrum. At these Fano antiresonances, a large transverse spin polarization can be crea

Popular Abstract in Swedish I avhandlingen behandlas tre texter av den tjeckiske författaren Bohumil Hrabal (1914–1997): ”Mrtvomat” (från 1949), Denna stad är i invånarnas gemensamma vård (från 1967) och ”Legend spelad på strängar spända mellan vaggan och kistan” (från 1968). Alla tre texterna är sammanställda av textsegment valda ur andra författares verk och av fragment av avlyssnade samtal. HraThe main theme of the thesis is an analysis of three texts by the Czech author Bohumil Hrabal (1914 – 1997), namely ’Mrtvomat’ (’Mrtvomat’, 1949), This City is in the Joint Care of Its Inhabitants (Toto mìsto je ve spoleèné péèi obyvatel, 1967), and ’Legend Played on Strings Stretched Between the Cradle and the Coffin’ (’Legenda zahraná na strunách mezi kolébkou a rakví’, 1968). These texts are co

Denna rapport behandlar fuktskador i ett hus uppfört 1973 med platta på mark och flytande golv. Mätningar har genomförts i huset under två år. Syftet var att erhålla mätdata under en lång tidsperiod i en byggnad med klara fukt- och luktproblem. I rapporten diskuteras lämpliga åtgärder för att komma till rätta med problemen.

In this study, we show that distinct compartmentalization patterns of the IL-1 molecules (IL-1alpha and IL-1beta), in the milieu of tumor cells that produce them, differentially affect the malignant process. Active forms of IL-1, namely precursor IL-1alpha (pIL-1alpha), mature IL-1beta (mIL-1beta), and mIL-1beta fused to a signal sequence (ssIL-1beta), were transfected into an established fibrosar

Context: T-3 action in neurons is essential for brain development. Recent evidence indicates that monocarboxylate transporter 8 (MCT8) is important for neuronal T-3 uptake. Hemizygous mutations have been identified in the X-linked MCT8 gene in boys with severe psychomotor retardation and elevated serum T-3 levels. Objective: The objective of this study was to determine the functional consequences

Fast line relaying algorithms are motivated by the need to keep the fault clearing time as short as possible. Fast fault clearing reduces the risk for transient instability, thermal stress on equipment and risk for personnel injuries. There exists, at least, three different themes in transmission line protection: impedance algorithms differential equation algorithms (DEA); and travelling wave algo

Problem med mikrobiell påväxt på fasader har ökat kraftigt på senare tid. Redan något år efter putsningen av en fasad har kraftig påväxt kunnat konstateras. En pilotstudie har genomförts av LTH i samverkan med JM AB och NCC Teknik, med stöd av SBUF och den visar att mögelpåväxten har samband med t ex fasadens utformning, ytans struktur, väggens värmeisolering och framför allt dess värmekapacitet.

Cubomedusae, or box jellyfish, have the most elaborate visual system of all cnidarians. They have 24 eyes of four morphological types, distributed on four sensory structures called rhopalia. Box jellyfish also display complex, probably visually guided behaviors such as obstacle avoidance and fast directional swimming. Here we describe the strikingly complex and partially bilaterally symmetrical ne

We analyzed the reactivity of three different commercially available rat monoclonal antibodies raised against mouse laminin-a1 beta 1 gamma 1 (laminin-11), AL-1, AL-2, and AL-4. Using ELISA assays, Western blot analysis and inummostainings we present refined epitope maps for these three laminin monoclonals. AL-1 reacted, as predicted with laminin alpha 1 chain. AL-4 has also been marketed as an al

Endoglucanases (EGI) (endo-1,4-β-d-glucan-4-glucanohydrolase, EC 3.2.1.4, Cel7B) of Trichoderma reesei are industrially important enzymes. Thus, there is a great need for development of a primary recovery method suitable for large-scale utilization. In this study we present a concept applicable for large-scale purification of an EGI fusion protein by one-step extraction in a poly(ethylene glycol)

We analyze the impact of the matching network on compact multiple-input multiple-output systems. Existing studies have found that the matching network has a significant influence on the performance of multiple antenna systems when the antennas are in close proximity. However, none has examined the wide-band case. In this paper, we investigate the wide-band performance of four different matching ne

My thesis is an attempt to give a minimalist account of word formation in Swedish. (See Chomsky (1993) and (1994).) In the main part of my thesis I demonstrate how the proposed system accounts for the central aspects of Swedish word formation. Simple words, compounds, and derivations alike are described as formed by Generalized Transformations, primarily by Merge, but also by Move. From a lexicon

A series of proteins and one membrane-bound peptide have been partitioned in aqueous two-phase systems consisting of micelle-forming block copolymers from the family of Pluronic block copolymers as one polymer component and dextran T500 as the other component. The Pluronic molecule is a triblock copolymer of the type PEO-PPO-PEO, where PEO and PPO are poly(ethylene oxide) and poly(propylene oxide)

Previously the screening of tropical plants demonstrated a high peroxidase activity in sweet potato (Ipomoea batatas) tubers. The major peroxidase pool is localized in peel. Using peel of sweet potato as a source, the sweet potato peroxidase (SPP) has been isolated and purified to homogeneity. The enzyme purification included homogenization, extraction of colored compounds and consecutive chromato

Molecular engineering of ligand-binding proteins is commonly used for identification of variants that display novel specificities. Using this approach to introduce novel specificities into CBMs (carbohydrate-binding modules) has not been extensively explored. Here, we report the engineering of a CBM, CBM42 from the Rhodothermits marinus xylanase Xyn10A, and the identification of the X-2 variant. A

The clinical course in urinary bladder cancer is difficult or impossible to predict based on conventional disease parameters. It is a reasonable hypothesis that the genetic aberrations acquired by the tumor cells, being instrumental in bringing about the disease in the first place, may also hold the key to more reliable prognostication. However, though 200 transitional cell carcinomas (TCC), the m

Rhizotrons provide valuable information about plant root production, but measurements are usually made in units of root length per unit surface area of observation window surface. These measurement units are not easily comparable to above-ground plant growth. To address this deficiency, several techniques have been developed to convert rhizotron measurement units into root mass production per unit