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Engineering of a novel chimera of superoxide dismutase and Vitreoscilla hemoglobin for rapid detoxification of reactive oxygen species

The genes encoding human manganese superoxide dismutase (MnSOD) and Vitreoscilla hemoglobin (VHb) were fused inframe to generate a bifunctional enzyme that possessed MnSOD and peroxidase-like activities. At neutral pH, the coupling of the SOD and peroxidase reactions revealed that the bifunctional enzyme exhibited a 2.5 times shorter transient period and a 1.67 times higher reaction rate at steady

Past food habit change is related to obesity, lifestyle and socio-economic factors in the Malmo Diet and Cancer Cohort.

Objectives: To examine if obesity status and socio-economic and lifestyle factors are associated with self-reported past food habit change, and also whether the level of obesity depends on the reason for change. Design: Cross-sectional analysis within the Malmo Diet and Cancer (MDC) study using data from the baseline examination and the extensive socio-economic and lifestyle questionnaire includin

Glued-in rods for timber structures - a 3D model and finite element parameter studies

A nonlinear 3D finite element model and a theoretical parameter study in relation to glued-in rods for timber structures are presented. A strain-softening crack band model was used to characterise the behaviour of the adhesive layer between the rod and the wood. The model is general in the sense that it bridges the gap between the theory of an ideal plastic bondline and the theory of linear elasti

Annual dynamics of reedbed passerines in Ria de Aveiro, Portugal, assessed by standardized mist-netting.

Seventy standardized ringing sessions were carried out from April 1997 to August 2000 in a reedbed in Ria de Aveiro at Salreu, Portugal. The variation in the abundance of the common species is described as well as some aspects of their weight dynamics. Both, number of birds and species were higher during summer and autumn, when passage migrants occur and the number of juveniles is high. The pre-br

Offspring genetic structure reveals mating and nest infestation behaviour of an invasive parasitic fly (Philornis downsi) of Galápagos birds

The natural reproductive behaviour of invasive insects is pivotal knowledge for managing species of ecological or economic concern. We use microsatellites to examine female multiple mating and multiple nest infestations in the introduced parasitic fly, Philornis downsi, which causes high mortality in endemic birds on the Galápagos Islands. We analyse larvae and pupae within 57 nests from Santa Cru

Economic Evaluation of Smoking-Cessation Therapies: A Critical and Systematic Review of Simulation Models.

Background: Smoking is probably the most important among preventable health risks. Health economic evaluation of smoking-cessation interventions, applying a lifetime perspective, is made possible by available epidemiological knowledge. The well established method of performing cost-effectiveness analyses of smoking-cessation interventions involves mathematical modelling (both deterministic and sto

Depletion of myocardial glucose is observed during endotoxemic but not hemorrhagic shock in a porcine model

Introduction Metabolic dysfunction is one of the hallmarks of sepsis yet little is known about local changes in key organs such as the heart. The aim of this study was to compare myocardial metabolic changes by direct measurements of substrates, such as glucose, lactate and pyruvate, using microdialysis (MD) in in-vivo porcine endotoxemic and hemorrhagic shock. To assess whether these changes were

Två generationer hyfsad småländska

The author (professor of Scandinavian linguistics, *1934, living in Lund or nearby) compares his own dialect with that of his mother (house-wife, *1912, living nearly all her life 15 kilometers outside Kalmar). Interestingly, he found very few differences. The article discusses why.

Analytical Gradients of Hartree-Fock Exchange with Density Fitting Approximations

We extend the local exchange (LK) algorithm [Aquilante, F.; Pedersen, T. B.; Lindh, R. J. Chem. Phys. 2007, 126, 194106] to the calculation of analytical gradients with density fitting. We discuss the features of the screening procedure and demonstrate the possible advantages of using this formulation, which is easily interfaced to a standard integral-direct gradient code. With auxiliary basis set

Production of prebiotic xylooligosaccharides from alkaline extracted wheat straw using the K80R-variant of a thermostable alkali-tolerant xylanase

Agricultural by-products are raw materials of importance for increased utilization of renewable biomass. Wheat straw is a raw material of significant production volume and is in this work used for production of xylooligosaccharides (XOS). Extraction of xylan by dilute alkali was followed by hydrolysis using a variant of the alkali-tolerant Bacillus halodurans S7 endoxylanase A mutated at K80R. The

A modified level set approach to 2D modeling of dynamic recrystallization

The macroscopic properties of metallic materials depend on the state of the grain microstructure. Recrystallization acts as one of the most important mechanisms in the evolution of the microstructure and hence also of the macroscopic properties. This paper presents a mesoscale model of microstructure evolution due to recrystallization, based on a level set formulation employed in a finite element

17α-Ethinylestradiol (EE2) treatment of wild roach (Rutilus rutilus) during early life development disrupts expression of genes directly involved in the feedback cycle of estrogen.

Fish are more sensitive to introduced disturbances from synthetic endocrine disrupting compounds during early life phases compared with mature stages. 17α-Ethinylestradiol (EE2), which is the active compound in human oral contraceptives and hormone replacement therapies, is today ever present in the effluents from sewage treatment plants. EE2 targets and interacts with the endogenous biological sy