Målet med detta arbete var att rena fram proteinet Chlamydia trachomatis ribonucleotide reductase R1 Δ248. För att göra detta effektivt, med en så liten förlust av proteinet som möjligt under processen, så krävs kunskap om proteinets egenskaper. Med vetskapen att denna mutant, som saknar de första 248 aminosyrorna jämfört med den icke-muterade varianten, enbart har en ATP-kon medan den icke-muteraThe enzyme Ribonucleotide Reductase (RNR) reduces ribonucleotides (NTPs) to deoxyribonucleotides (dNTPs), which are then used as building blocks in the synthesis of DNA. In this thesis are reported the expression and purification of a mutant version of Chlamydia trachomatis Ribonucleotide Reductase protein R1 , called Chlamydia trachomatis Ribonucleotide Reductase protein R1 Δ248. The R1 subunit o

STI (Soil Topografic Index) är ett hydrologiskt index som beskriver markfuktigheten i ett landskap. Då indexet är baserat på de relativt konstanta faktorerna topografi, jordens textur och jorddjup beskriver det ett generellt markfuktighetsmönster. Indexet applicerades på jordbruksmark i Helsingborgs kommun för att få kunskap om vilka områden markfuktigheten tenderar att vara låg eller hög. Vid extThe index STI describes water flow path in a landscape. Whit the help of STI it´s possible to detect areas where the soil moisture is expected to be very high or low. When comparing the result of the STI for the chosen study-area with interviews for farmers in the same area, where was a similarity between which areas the STI identified as risk for high soil moisture and which areas the farmers ide

Background Depressive disorders, specifically major depressive disorder (MDD), is predicted to become the leading cause of disease burden worldwide by 2030. In the United States, MDD is already the leading cause of disability. Yet, less than 50 percent of US adults diagnosed with a mental disorder seek treatment, often due to lack of health coverage or affordability. In 2014, the Affordable Care A

The drastic changes in infrastructure and arrival of luxury tourism to South Sinai has brought the global world at the doorstep of the region’s nomadic bedouins. The ever-expanding global influence has given birth to a generation of young bedouin women who absorb the surrounding new ideals, behaviors and understandings, blending them with their tribal culture. This has created identity hybridities

Climate changes have a large impact on precipitation patterns and precipitation variability change. With this in mind, precipitation over Greenland is studied. This is done for present day precipitation model data from 1991-2010 compared to future model simulations of precipitation for 2081-2100. Furthermore, precipitation drives Surface Mass Balance (SMB), which is the sum of accumulation and the

Hösten 2017 startade uppropet #tystnadtagning för att uppmärksamma skådespelerskors upplevelser av sexuella trakasserier i arbetet. Genom flertalet berättelser delade skådespelerskorna i uppropet med sig av personligt upplevda sexuella kränkningar i syftet att förändra sin arbetssituation. Denna uppsats syfte är att generera större branschspecifik kunskap om sexuella trakasserier i arbetet ur kvin

Purpose: The ambition of this study is to update the underlying assumptions about the role of a brand manager in the new digital era by illuminating how the role is executed and perceived by brand managers themselves. This study aims to update the language concerning a brand manager’s role with the help of metaphors and also, problematize the traditional assumptions regarding brand management. Me

Based on an ethnographic case study, this paper offers a typology of how, and under what circumstances, employees at a fast food-restaurant are cynical. By conducting participant- and non-participant observations and 10 semi-structured interviews, combined with a document analysis, we sought to understand the social realities of employees at Berger. Thus, we have had an interpretive approach to re

In July 2017, the Egyptian police and military forces implemented a government decision that intended to force the residents of al-Warraq island out of their homes. The eviction decision involved 720 homes, but the security forces were only able to demolish five homes before clashes broke out and the security forces withdrew from the island. The disputes between the police forces and island inhabi

This study aims to deduce what Japanese native speakers think of the Japanese writing systems, and in particular what native speakers’ opinions are concerning Kanji, the logographic writing system which consists of Chinese characters. The Japanese written language has something that most languages do not; namely​ a total of three writing systems. First, there is the Kana writing system, which cons

Populärvetenskaplig beskrivning: Att veta hur olika saker i världen kom till hjälper en att släcka den törstiga sömn-förstörande nyfikenheten. Många har ställt sig frågan: hur skapades universum? Någorlunda hela svar har lagts fram (som till exempel teorin om Big Bang), men det finns mer att upptäcka. På en relativt mindre skala kan man fråga sig hur skapades Vintergatan? Ännu en fråga delvis svaradThe properties of the early Milky Way are quite mysterious and some are completely unknown even today. This study is the first one ever to analyze the mass distribution of Population III bulge stars. Using the fitting code STARFIT, we perform an analysis of 65 bulge metal-poor stars obtained by the EMBLA survey to estimate the IMF (initial mass function) of the very first stars that defined our ga

Artificiella neuronnät är en enkel matematisk maskininlärningsmodell som på vissa sätt påminner om de neuronnät som återfinns hos djur. Syftet med maskininlärning är att inte behöva programmera exakt hur en maskin ska lösa en uppgift eftersom detta i vissa fall kan vara väldigt svårt eller näst intill omöjligt. Istället låter man maskinen lära sig utifrån erfarenhet av att utföra en uppgift. DennaThe recently introduced capsule networks have already shown much success on image classification tasks. In this thesis, capsule networks are described and applied to a binary classification task, namely that of classifying hotspots from bone scintigraphy scans. The performance of capsule networks on this task is compared to that of convolutional neural networks. The results indicate that there is

The paper investigates the financing behavior of firms listed on the Stockholm Stock Exchange (SSE) main markets using the pecking order theory. The study also examines the influence of institutional and insider ownership identities on the financing decisions of the firms. Examining a sample of 107 firms from 2009-2017, we found the pecking order theory of corporate financing consistent with firms

This thesis aims to determine whether there is a human right to education in mother tongue for national minorities in international as well as regional human rights law. Its goal is to contribute to the existing research by evaluating and assessing the available treaties as well as non-binding documents issued by United Nations, Council of Europe and Organization for Security and Co-operation in E

Urbanisation in Beirut has led to a situation where the city suffers from a severe lack of green public space—Lebanon’s capital have ended up with some of the least amount of public green space per capita in the world. The lack of green surfaces and vegetation in Beirut contributes to the city’s Urban Heat Island (UHI)—a phenomenon where an urban or metropolitan area traps waste heat and end up si

I Sverige är en stor del av byggnadsbeståndet uppfört före 1973. Detta år har stor betydelse då vi detta år hade en oljekris som ändrade inställningen till energi och byggande. Sverige ligger i norra delen av Europa och har en låg medeltemperatur jämfört med stora delar av övriga Europa. Detta har gjort att det ställts hårda krav på modernt byggande som stramas åt med jämna mellanrum. De gamla byg

I Sveriges storstadsregioner råder idag en brist på bostäder. Vill man hyra en lägenhet ska man helst ha ställt sig i kö många år i förväg. Har man inte kötid, kontakter eller lust att betala ockerhyra för att bo i andra hand bör man ha en årsinkomst eller två i kontanter bara för att få låna ihop till en bostadsrätt. Till följd av detta byggs det nu mer än på länge, men det som byggs är oftast inIn Sweden, there’s currently a shortage of housing. If you want to rent an apartment, you will have to have been queuing for years. If you don’t have the queue time to compete, the necessary contacts or you just don’t want to pay an arm and a leg for a sub-let you might need to have a year’s salary in cash just to be able to get the loan you need for a condominium. In reaction to this we are seein