BACKGROUND: Novel biomarkers are required to discern between breast tumors that should be targeted for treatment from those that would never become clinically apparent and/or life threatening for patients. Moreover, therapeutics that specifically target breast cancer (BC) cells with tumor-initiating capacity to prevent recurrence are an unmet need. We investigated the clinical importance of LGR5 i

In contrast to static objects, liquid structures such as drops, blobs, as well as waves and ripples on water surfaces are challenging to image in 3D due to two main reasons: First, the transient nature of those phenomena requires snapshot imaging that is fast enough to freeze the motion of the liquid. Second, the transparency of liquids and the specular reflections from their surfaces induce compl

There is an extensive research that explores the reproduction of transnational communities and relations, with a particular focus on locality, identity and culture. However, the existing research emanates from the case studies of immigrant communities in Western-type democracies, while we know little about migrant transnationalism in non-Western migration regimes such as Russia, where migrant work

BACKGROUND: This study aimed to identify factors associated with health-related quality of life (HRQoL) and the burden on the relatives of older people with multi-morbidity.METHODS: A secondary analysis of baseline data from 296 dyads, including older patients with multimorbidity and their relatives, which were previously collected in a randomized study. The analysis was conducted to select correl

Human carbonic anhydrase IX (CA IX) expression is upregulated in hypoxic solid tumours, promoting cell survival and metastasis. This observation has made CA IX a target for the development of CA isoform-selective inhibitors. To enable structural studies of CA IX-inhibitor complexes using X-ray and neutron crystallography, a CA IX surface variant (CA IXSV; the catalytic domain with six surface amin

Background: Currently, hand-held gamma cameras are being developed for 99mTc imaging, mainly for sentinel lymph node detection. These cameras offer advantages, such as mobility and ease of access, and may be useful also for other applications such as biokinetic studies in animals or for imaging of small, superficial structures in patients. In this work, the suitability of a CZT-based hand-held cam

The first measurement at the LHC of charge-dependent directed flow (v1) relative to the spectator plane is presented for Pb-Pb collisions at sNN=5.02 TeV. Results are reported for charged hadrons and D0 mesons for the transverse momentum intervals pT>0.2 GeV/c and 3

Cell division is essential for organismal growth and tissue homeostasis. It is exceptionally significant in tissues chronically exposed to intrinsic and external damage, like the liver. After decades of studying the regulation of cell cycle by extracellular signals, there are still gaps in our knowledge on how these two interact with metabolic pathways in vivo. Studying the cross-talk of these pat

This chapter describes a protocol for the separation of different cell fractions from Streptococcus pyogenes and sample preparation for analysis by mass spectrometry. The presented approach can be used for the analysis of all subcellular proteomes from S. pyogenes and enables identification of drug or vaccine targets within a certain cellular fraction. Here, proteins integral to the plasma membran

This paper presents a topology optimization framework for designing periodic viscoplastic microstructures under finite deformation. To demonstrate the framework, microstructures with tailored macroscopic mechanical properties, e.g., maximum viscoplastic energy absorption and prescribed zero contraction, are designed. The simulated macroscopic properties are obtained via homogenization wherein the

We propose to combine population-based register data, with a nested clinical cohort to correct misclassification and unmeasured confounding through probabilistic quantification of bias. We illustrate this approach by estimating the association between knee osteoarthritis and mortality. We used the Swedish Population Register to include all persons resident in the Skåne region in 2008 and assessed

Adrenomedullin is a potent vasodilatory peptide, linked to pulmonary arterial hypertension pathology. Proximity extension assays were utilized to study plasma biomarkers related to vasoregulation, with focus on adrenomedullin peptides and precursor levels, collectively referred to as ADM. ADM was measured in 48 treatment-naïve pulmonary arterial hypertension patients at diagnosis, and in 31 of the

We consider thermal fluid structure interaction with a partitioned approach, where typically, a finite volume and a finite element code would be coupled. As a model problem, we consider two coupled Poisson problems with heat conductivities $$\lambda _1$$, $$\lambda _2$$ in one dimension on intervals of length $$l:1$$ and $$l:2$$. Hereby, we consider linear discretizations on arbitrary meshes, such

The condition assessment of bridges considers a combination of information from different sources rendering multiple levels of assessment possible. This paper illustrates how successive condition assessment strategies increase the expected utility compared to single choice decisions through Bayesian inference. Multiple levels of assessment allow for additional possibilities for obtaining structura

Anisotropic macromolecules exposed to non-equilibrium (active) noise are very common in biological systems, and an accurate understanding of their anisotropic dynamics is therefore crucial. Here, we experimentally investigate the dynamics of isolated chains assembled from magnetic microparticles at a liquid-air interface and moving in an active bath consisting of motile E. coli bacteria. We invest

Shiga toxin (Stx)-stimulated blood cells shed extracellular vesicles (EVs) which can transfer the toxin to the kidneys and lead to hemolytic uremic syndrome. The toxin can be taken up by renal cells within EVs wherein the toxin is released, ultimately leading to cell death. The mechanism by which Stx is taken up, translocated, and sequestered in EVs was addressed in this study utilizing the B-subu