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In hereditary cystatin C amyloid angiopathy (HCCAA), presence of the Leu68 Gln substitution in cystatin C is coupled to a decreased concentration of this major cysteine proteinase inhibitor in cerebrospinal fluid and leads to its amyloid deposition in the brain. We established a high-yield expression system for L68Q cystatin C in Escherichia coli resulting in inclusion body accumulation at a level
C-CAM (Cell-CAM 105) is a cell surface glycoprotein that is involved in cell-cell adhesion of rat hepatocytes in vitro. To elucidate the adhesion mechanism the binding properties of purified C-CAM were investigated. Using proteins immobilized on nitrocellulose it was found that radiolabeled C-CAM bound to C-CAM but not to a variety of other proteins. Partitioning in Triton X-114 showed that C-CAM
The physical nature of water and the environment it presents to an organism have long been recognized as important constraints on aquatic adaptation and evolution. Little is known about the dermal cover of mosasauroids (a group of secondarily aquatic reptiles that occupied a wide array of predatory niches in the Cretaceous marine ecosystems 92-65 Myr ago), a lack of information that has hindered i
A new method is proposed for the computation of CO2 Net Ecosystem Exchange (NEE) and its components in a forest ecosystem. Advective flux is estimated by taking into account the air mass conservation principle. For this purpose, wind and dry air density values on the surface of the control volume are first corrected and then the advective flux is estimated on the surface of the control volume. Tur
Oocytes obtained from 85 women undergoing IVF/intracytoplasmic sperm injection (ICSI) treatment were randomly selected for culture in media containing either human serum albumin (HSA), (n = 42) or recombinant human albumin (rHA), (n = 43). At the time of transfer, the two embryos with the overall best morphology were selected on day 2, 3 or 5. Comparable rates of fertilization, cleavage, blastocys
The hottest developments in the fields of cancer research, neuroscience, genomics and proteomics, anti-virals and more, with a pick of the key research papers in these areas.
The lifetime of the metastable level 3d(21)G(4) in a singly charged scandium has been experimentally investigated at the ion storage ring CRYRING. A laser probing technique has been used for the studies. We also report calculated lifetimes and transition rates for 10 metastable levels in Sc II, including the 3d(21)G(4) state, by calculations using configuration interaction wave functions. The life
Recent findings in adult rodents have provided evidence for the formation of new striatal neurons from subventricular zone (SVZ) precursors following stroke. Little is known about which factors determine the magnitude of striatal neurogenesis in the damaged brain. Here we studied striatal neurogenesis following an excitotoxic lesion to the adult rat striatum induced by intrastriatal quinolinic aci
This study was part of a Swedish interdisciplinary research project targeting accessibility problems in public transport for people with cognitive functional limitations (CFLs). The objective was to describe and compare different assessment perspectives of long-term CFLs among community citizens having had CFLs in the acute stroke phase but with moderate physical limitations. Eighty-four participa
A novel and a simple method that allows direct imaging of viscous samples by cryo-TEM (cryo-transmission electron microscopy) is presented. A fracture on the vitrified sample is created in a controlled way. In the fracture, some edges are thin enough to allow direct imaging in transmission mode. The method was used to directly image a nonionic surfactant lamellar phase where a random mesh structur
Recent comparative observations of long-duration gamma-ray bursts (LGRBs) and core collapse supernova (cc SN) host galaxies demonstrate that these two, highly energetic transient events are distributed very differently upon their hosts. LGRBs are much more concentrated on their host galaxy light than cc SN. Here we explore the suggestion that this differing distribution reflects different progenit
We present a versatile and compact fiber optic probe for real-time determination of the absorption and the reduced scattering coefficients from spatially resolved continuous wave diffuse reflectance measurements. The probe collects the diffuse reflectance at six distances in the range 0.6 - 7.8 mm at four arbitrary wavelengths, which were 660, 785, 805, and 974 nm in these experiments. The maximum
A growing body of evidence points out the potential role of inflammatory mechanisms in the pathophysiology of brain damage in dementia. In previous studies, we have demonstrated intrathecal production of the proinflammatory cytokine tumor necrosis factor (TNF)alpha in patients with Alzheimer's disease (AD). The aim of the present study was to investigate the downstream products of TNF-alpha expres
Link dimensioning and routing problems in resilient network design are considered. Reliable network operation is ensured by means of flow restoration which is performed on preselected protection (backup) paths that can absorb traffic overflows from failed primary paths. Backup and primary flows use separated link capacities, and can be split among many paths. In the paper, two restoration models a
The aim of the study was to investigate the main and the interaction effects of the demand-control-support (DCS) model on women’s and men’s health in a Swedish telecom company. According to the DCS model, work that is characterized by high demands, low decision latitude, and low support decreases health and well-being. Furthermore, control and support are assumed to interact in protecting against
The results on the extraction of natural compounds from Agrimonia eupatoria and Agrimonia procera by using supercritical carbon dioxide (SC-CO2) are presented. Two agrimony species were extracted in laboratory scale equipment at various parameters and it was found that in case of A. eupatoria the increase in extraction pressure and the addition of a co-solvent ethanol remarkably increases the yiel
In eukaryotes, the number and rough organization of chromosomes is well preserved within isolates of the same species. Novel chromosomes and loss of chromosomes are infrequent and usually associated with pathological events. Here, we analyzed 40 pathogenic isolates of a haploid and asexual yeast, Candida glabrata, for their genome structure and stability. This organism has recently become the seco
For the measurement of rare gamma decays with low gamma-ray multiplicities (M-gamma < 15) dedicated Ge-detector arrays are being developed. They are optimized with respect to the full-energy peak efficiency rather than the resolving power. These so-called miniball arrays will pre dominantly be used at radioactive ion beam facilities. The detector arrays will have a high granularity using segmented
Illumination of a chick’s eye allows light to pass through to the retina of the contralateral eye. Electroretinographic (ERG) recording employing the scalp or comb as a reference results in shorter implicit time, higher amplitude and lower sensitivity during the day than during the night in a light:dark (LD) cycle and in constant darkness (DD). ERG recordings employing the contralateral eye as ref
