We provide evidence that the human papillomavirus (HPV) E2 protein regulates HPV late gene expression. High levels of E2 caused a read-through at the early polyadenylation signal pAE into the late region of the HPV genome, thereby inducing expression of L1 and L2 mRNAs. This is a conserved property of E2 of both mucosal and cutaneous HPV types. Induction could be reversed by high levels of HPV-16

The coiled-coil protein DivIVA is a determinant of apical growth and hyphal branching in Streptomyces coelicolor. We have investigated the properties of this protein and the involvement of different domains in its essential function and subcellular targeting. In S. coelicolor cell extracts, DivIVA was present as large oligomeric complexes that were not strongly membrane associated. The purified pr

Recension av Olle Östlind, Essäer om Henry B. Goodwin.

Background: Over the past quarter century, the incidence of tick-borne encephalitis (TBE) has increased in most European nations. However, the number of humans stricken by the disease varies from year to year. A method for predicting major increases and decreases is needed. Methods: We assembled a 25-y database (1984-2008) of the number of human TBE victims and wildlife and climate data for the St

The concentration of cholesterol in cell membranes affects membrane fluidity and thickness, and might regulate different processes such as the formation of lipid rafts. Since interpreting experimental data from biological membranes is rather intricate, investigations on simple models with biological relevance are necessary to understand the natural systems. We study the effect of cholesterol on th

The Swedish reimbursement system operates a system where prices are set based on the expected value to the consumer. This value can be measured using willingness to pay (WTP).

Human pluripotent stem cells possess the potential to generate cells for regenerative therapies in patients with neurodegenerative diseases, and constitute an excellent cell source for studying human neural development and disease modeling. Protocols for neural differentiation of human pluripotent stem cells have undergone significant progress during recent years, allowing for rapid and synchroniz

This thesis is based on five studies. It includes the characterization of lipid micro emboli in shed mediastinal blood during cardiac surgery. The reuse of blood directly by cardiotomy suction is a common practice in order to reduce the need of blood transfusions. In shed mediastinal blood we showed that there is an abundance of lipid particles in the size range of 10-60μm. These lipid particles a

Two (Ga,Mn)As samples having different magnetic anisotropy (one with in-plane easy axis and another one with out-of-plane easy axis) were studied by means of magnetotransport experiments. Anisotropy field B-A was determined for both samples as a function of temperature. For the sample having in-plane easy axis, an inversion of the direction of planar Hall effect hysteresis was observed upon increa

Context Software startups are newly created companies with no operating history and fast in producing cutting-edge technologies. These companies develop software under highly uncertain conditions, tackling fast-growing markets under severe lack of resources. Therefore, software startups present a unique combination of characteristics which pose several challenges to software development activitie

Amplification of 8p12-p11 is relatively common in breast cancer and several genes within the region have been suggested to affect breast tumor progression. The aim of the study was to map the amplified 8p12-p11 region in a large set of breast tumors in an effort to identify the genetic driver and to explore its impact on tumor progression and prognosis. Copy number alterations (CNAs) were mapped i

This dissertation looks into the treatment of exposed children and young people. The background is a puzzlement as to how the discourse on treatment, during the latest twenty years, has concentrated on the same institutions and themes (Ebsen, 2007). The aim of the dissertation is to shed light on how the institutions of child saving, (family care, placement in residential care, intervention in the

How would a low-carbon energy transformation affect energy security? This paper proposes a frame- work to evaluate energy security under long-term energy scenarios generated by integrated assessment models. Energy security is defined as low vulnerability of vital energy systems, delineated along geographic and sectoral boundaries. The proposed framework considers vulnerability as a combination of

Geothermal energy is classified as a renewable energy source and it utilizes the heat generated in the earth primarily from the natural radioactive decay of isotopes of uranium, thorium and potassium. Heat is extracted from the earth to generate geothermal energy via a carrier, usually water occurring either in the liquid or steam phase. In the late 19th century and the early 20th century, the fir

Neuroblastomas (NBs) are tumours of the sympathetic nervous system accounting for 8-10% of paediatric cancers. NBs exhibit extensive intertumour genetic heterogeneity, but their extent of intratumour genetic diversity has remained unexplored. We aimed to assess intratumour genetic variation in NBs with a focus on whole chromosome changes and their underlying mechanism. Allelic ratios obtained by S

OBJECTIVE-Age at menopause is an important determinant of future health outcomes, but little is known about its relationship with type 2 diabetes. We examined the associations of menopausal age and reproductive life span (menopausal age minus menarcheal age) with diabetes risk. RESEARCH DESIGN AND METHODS-Data were obtained from the InterAct study, a prospective case-cohort study nested within the

A numerical model that combines a random wave transformation and a wave-induced current model was developed in order to predict the wave and current fields around a detached breakwater. The wave field was determined using the EBED model, as reported by Mase in 2001, with a modified energy dissipation term. The surface roller associated with wave breaking was modeled based on a modification of the

An electrochemical biosensor based on ZnO nanorods for potentiometric cholesterol determination is proposed. Hexagon-shaped ZnO nanorods were directly grown on a silver wire having a diameter of 250 mu m using low temperature aqueous chemical approach that produced ZnO nanorods with a diameter of 125250 nm and a length of similar to 1 mu m. Cholesterol oxidase (ChOx) was immobilized by a physical

Popular Abstract in Swedish Om blodkärlen i ögat läcker eller om blodflödet stoppas får inte näthinnan den mängd syre och näring som den behöver. Detta tillstånd kallas ischemi och kan leda till att näthinnans celler skadas. Näthinnan ligger längst bak i ögat och fungerar som filmen i en kamera. Stavar och tappar registrerar ljus och signalen går via nerver till syncentra i hjärnan. Blodkärl (artäIdentification of the intracellular signal transduction pathways activated in retinal ischaemia may be important in revealing novel pharmacological targets. The retinal blood vessels are key organs in circulatory failure, and in this work the retinal vasculature was therefore examined separately from the neuroretina. The porcine eye has a typical primate-like architecture and is similar to the hum

The ATLAS experiment at the LHC has measured the centrality dependence of charged particle pseudorapidity distributions over vertical bar eta vertical bar < 2 in lead-lead collisions at a nucleon-nucleon centre-of-mass energy of root s(NN) = 2.76 TeV. In order to include particles with transverse momentum as low as 30 MeV, the data were recorded with the central solenoid magnet off. Charged partic