USING CRISPR/Cas9 TO DELETE REDUCTASES IN YEAST TOWARDS IMPROVED CAPSAICINOID BIOSYNTHESIS
The baker’s yeast, Saccharomyces cerevisiae (S. cerevisiae) was studied and genetically engineered. This was to enhance the transamination step of the biochemical reaction cascade towards the production of capsaicin. Inherent reductases were identified and twelve of them knocked out via homologous recombination. Using the CRISPR/Cas9 technology, the Green Fluorescence Protein (GFP) gene was introd