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USING CRISPR/Cas9 TO DELETE REDUCTASES IN YEAST TOWARDS IMPROVED CAPSAICINOID BIOSYNTHESIS

The baker’s yeast, Saccharomyces cerevisiae (S. cerevisiae) was studied and genetically engineered. This was to enhance the transamination step of the biochemical reaction cascade towards the production of capsaicin. Inherent reductases were identified and twelve of them knocked out via homologous recombination. Using the CRISPR/Cas9 technology, the Green Fluorescence Protein (GFP) gene was introd

A Novel Perceptual Metric in Deep Learning

Förmågan att återskapa förvrängda bilder på ett effektivt sätt har länge varit ett mål inom datorgrafik och bildanalys. I vårt examensarbete, i samarbete med NVIDIA, har vi tittat på ett nytt tillvägagångsätt för detta problem inom maskininlärning och jämfört detta med redan existerande metoder.Loss functions are a crucial part of image processing when using modern neural networks, trained with stochastic gradient descent. There exist multiple loss functions today, some claiming to be perceptual. Researchers at NVIDIA recently published a proposal of such a metric called FLIP. This led to our work on this thesis where we present a comparison between multiple loss functions, both establis

Kvinnliga missbrukares livsstilar : Behandlingsassistenters uppfattningar om identitet och sociala regler bland kvinnor som vårdas på LVM-hem.

The aim of this study was to explore how treatment assistants at a treatment home perceived the identity of the women cared for at the treatment home. The aim was also to explore how treatment assistants perceived the women’s approach to social rules. The method chosen for this study was qualitative interviews with five treatment assistants. The treatment assistants worked with compulsive care at

The story that I was never told

Winston Churchill once said “democracy is the worst form of government except for all those other forms that have been tried from time to time”. Can this sentence be related to economic growth and development? This thesis analyzes the economic outcomes of the two main political regimes (Democracy and Dictatorship) in the developing world and discovers that Churchill’s words cannot be applied for l

Principal Component Analyses of Final States in Relativistic Nuclear Collisions using PYTHIA/Angantyr

We develop a program to perform a principal component analysis of flow fluctuations using simulations of nuclear collisions produced by the PYTHIA/Angantyr event generator. We consider both p+p and p+Pb collision events and study the leading and subleading modes of the second flow harmonic. The analysis is carried out using two different definitions of the flow matrix which differ in their sensiti

“Korsar jag väg med fel creep så är det ju kört” En metodkombinerad studie om kvinnors upplevelse av säkerhet vid användande av Tinder/Badoo

This study aims to examine how women, between the ages 18-30, experience the safety during the usage of the dating apps Tinder/Badoo. The study also aims to examine which possible factors that can comprise the experience of safety during the usage. The reason behind this is based on the wide range that this type of dating has gotten over the past several years. Previous studies have mainly been fo

Bokens klimatavtryck - en undersökning av tre svenska förlags miljöarbete

Syftet med uppsatsen är att ta reda på hur de tre största bokförlagen i Sverige arbetar med miljöfrågor kring produktion av tryckta böcker. Uppsatsens underlag är hämtad från Bonnierförlagens, Norstedts och Natur & Kulturs hemsidor. Denna information kompletteras med intervjuer med representanter från förlagen samt ett antal personer med kunskap inom miljöfrågor. De teorier som används är legi

Med vapen som livförsäkring: en kvalitativ studie av vapeninnehav i Malmö

Sweden has in recent years seen an uprising in gun-use within socioeconomically weak areas and within gangs. Currently, political discourse circle around dissecting the matter of illegal weaponry attempting to find a way in which to curb deadly violence. This study aims to create an understanding of the mechanics between the law and the criminal sub-world with the help of looking at the effects of

What are the key enabling factors for sustainable innovative environment in healthcare?

Background: The more varied and new challenges that the healthcare sector faces every day, calls for modernization and reorganization. However, the healthcare sector is commonly understood to be slow to adapt to change. It is therefore important to understand and identify the enabling factors for the implementation of innovation, by giving voice to healthcare practitioners concerns, considering th

Hur många gånger tänker du bita i det sura äpplet? En kvalitativ fallstudie om varför kunder behåller sin varumärkeslojalitet efter en kris

This study aims to, from a sensemaking perspective, increase knowledge about under what conditions customers of a well-known high-involvement brand remain loyal to said brand post-crisis. It also aims to further contribute an understanding of how the level of brand loyalty affect the conditions under which customers remain loyal to a brand post-crisis. The empirical material is collected through 1

"För att du hör hemma där" - En studie om kvinnliga musiker på gymnasiets estetiska program

Denna studie syftar till att undersöka hur unga kvinnor, eller tjejer som jag valt att kalla dem, som studerar på gymnasiets estetiska program upplever sin vardag inom musiken. Musikbranschen är en ojämställd värld och syftet med denna studie är att identifiera skillnader och orättvisor för att musiklärare ska få större möjligheter att arbeta jämställt. Detta jämställdhetsarbete kan bidra till attThe aim of this study is to investigate how young females, or girls as I have choosen to call them, who study at the aesthetic program of high school, experience their everyday lives. The music industry is not equal and the purpose of this study is to identify the differences and injustice, in order to give the music teachers possibilities to work with equality. The work with equality can contribu

Strategic Planning in Small and Medium-sized Enterprises in Sweden

Small and medium-sized enterprises (SMEs) constitute an important part of the national economies. In order to be able to survive and compete with large businesses, SMEs have to actively plan their future. This research qualitatively examines strategic planning practises in SMEs in Sweden, a yet unexplored country in this matter, in order to identify their extent and understand their nature. For th

T-Cell Development: Transcriptional and Epigenetic Regulation in BCL11B Activation

Haematopoietic stem cells are the source of all blood components including T-cells. When the T-cell programme is initiated in these cells, the expression of T-cell associated genes such as TCF7 is up-regulated, while genes opposing this fate are turned off. Subsequently, epigenetic marks on the gene BCL11B and its regulatory elements, disabling the expression of the gene, are removed and the trans

Real-Time Monocular SLAM System Using an Inertial Measurement Unit to Estimate the Metric Scale and Gravity Direction

In this work a simultaneous localisation and mapping system, SLAM, has been constructed which can incorporate inertial measurement unit, IMU, data such as accelerometer and gyroscope data to estimate the metric scale and the relationship to the gravity vector of the SLAM solution. The system can perform accurate pose predictions at 100Hz based on IMU data and previous camera poses. An improved KLT

Unga vuxnas psykiska ohälsa

Studien undersökte sambanden mellan självkänsla, perfektionism, missnöje med utseende och kropp, upplevelsen av en inre eller yttre trygg plats samt psykisk ohälsa hos unga vuxna. Deltagarna var 122 unga vuxna (85% kvinnor, 12% män och 3% ej definierat kön) i åldrarna 18 till 23 år (median 20 år) som sökte de tre ungdomsmottagningarna i Malmö under två veckor i oktober, 2017. Deltagarna fick besvaThis study examined the correlations between self-esteem, perfectionism, dissatisfaction with physical appearance and body image, the experience of an inner and outer safe place, as well as mental illness among young adults. The study population consisted of 122 young adults (85 % women , 12 % men and 3 % of undefined gender) aged 18 -23 years old (median 20 years) seeking treatment at the Youth G

Gendering Normative Power Europe in practice: A study of the European Union’s implementation of the Women, Peace and Security-agenda

UNSCR 1325 and the Women, Peace and Security agenda has been in place since year 2000, yet women’s participation in conflict resolution processes remains low and they are still largely being excluded from deciding how their future society is going to look like. The European Union is known for being a normative actor in international politics, with gender equality being one of their core norms. As

Ung och bortglömd?: hur ungas aktörskap uttrycks i fredsprocesser

A major part of the world’s population today consists of young people, yet they are excluded from the process of peacebuilding as they are either demonised or infantilised. To address this issue the UN Security Council adopted resolution 2250 in 2015 which relates to youth, peace and security. This has led to an increase in attention of the positive role of youth for constructive change. The aim o

Den svenska modellen i gungning : en kritisk textanalys av Socialdemokraternas invandringsdiskurs 2013 och 2017

I studien granskas invandringsdiskursen i Sverige under en tid då högerextremism växer. Ett politiskt klimat som resulterat i ett hårdare språk och hårdare attityder mot flyktingar. Studien granskar förändringen i Socialdemokraternas språk vad gäller invandringsfrågan genom en undersökning av kongressprotokollen och de politiska riktlinjerna 2013 och 2017. Med hjälp av metodologiska verktyg, hämta

Neutral fred? : en analys av vilka mål och strategier som förespråkas för fredsbevarande operationer

Uppsatsen syftar till att undersöka vilken typ av fred som förespråkas i fredsbevarande operationer samt vilka metoder för att uppnå fred som premieras. För att avgränsa undersökningen har en specifik operation – FN:s insats i Mali, MINUSMA – valts ut. Primärmaterialet som analyseras är en rapport av svenska Totalförsvarets forskningsinstitut som utvärderar operationens resultat och utmaningar. Fr

"Lönen ser jag som att lära känna nya människor"

I denna uppsats har vi valt att undersöka hur elva studenter motiverar sitt engagemang i Lundakarnevalen. Frågeställningen som vi ämnar att besvara är följande: På vilket sätt motiverar studenter sitt ideella engagemang i Lundakarnevalen 2018? Denna studie inleds med tidigare forskning kring volontärarbete och motivation samt den kritik den bär med sig. Denna kritik argumenterar för vår teoretiska