FRET-studies of EF-hand complementation and the effect of conjugated fluorescent proteins
In this work spectroscopic methods have been used to determine the effect of fluorophores on protein affinity. Fluorophores are used in many different contexts to mark proteins, genes or antibodies. By marking a construct with a fluorophore, it is possible to follow the localization and function in a biological system, but it is not often investigated how the bound fluorophore could affect the mar