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Dispersed islet cells were prepared from collagenase-isolated lean mouse pancreatic islets by Dispase-II and subsequent mechanical treatment in calcium depleted media. An average yield of 600 cells per islet was obtained, 84% of the cells being β-cells. Cells were incubated with radioactive chromium as a marker of cell viability. Optimal labelling of 1-2 cpm per cell was obtained by incubating 10
Pancreatic islets from rats or ob/ob mice were homogenized and fractionated either by a two-step or one-step sucrose gradient centrifugation. A plasma membrane enriched fraction was obtained at a sucrose density of about 1.10. The distribution of the plasma membrane probe 125I-wheat germ agglutinin was parallel to that of other plasma membrane markers. Hydrolysis of Mg-ATP-gamma-3Pp in rat islet m
Rabbits were immunised with suspensions of viable, insulin-producing islet cells prepared from collagenase-isolated rat or ob/ob mouse pancreatic islets. Antibodies reactive with the surface of dispersed rat islet cells were present in both the rabbit anti-rat and the rabbit anti -ob/ob mouse islet sera as revealed by indirect immunofluorescence or by a radioligandassa
There is evidence that the destruction of the pancreatic B cell in insulin-dependent diabetes mellitus is due to autoimmune reactions. Transplantation of islet cell tissue might then provoke 'reimmunization', and this could presumably lead to destruction of the transplanted B cells. The authors have looked for signs of recurrence of autoimmunity following pancreatic transplantation.
Building on empirical material from 6 months ethnographically inspired fieldwork in Málaga Común, a mutual credit community currency in Southern Spain, the paper uses Ostrom’s (1991) theoretical framework on common-pool resources to look deeper into the provision and appropriation dynamics in the currency scheme. Particular attention is put into the sources of inequality in members’ provision and
Sweden went back to gold at the pre‐war parity, whereas Finland returned at the existing rate, accepting the depreciation of the markka that had occurred over the war and early post‐war years. The authors use a quantity‐theory framework to investigate the results of the divergent exchange rate and monetary policies followed by the two countries. The Swedish economy was forced to undergo a severe c
This study investigates domestic cat vocalisations in different contexts and mental states. Measures of fundamental frequency (f0) and duration as well as f0 contours of 780 meows from 40 cats were analysed. We found significant effects of recording context and of mental state on f0 and duration. Additionally, contours in positive (affiliative) contexts and mental states were predominantly rising,
En matière de proximité, la gouvernance des déchets ménagers en Suède repose sur un enchevêtrement de logiques politique, administrative, géographique, économique, juridique, organisationnelle et pratique. Ces logiques sont parfois complémentaires, parfois contradictoires. Selon que le déchet relève ou non d’un système de responsabilité élargie des producteurs, selon qu’il est produit ou non dans
