Determination of reducing ends with flow injection analysis with amperometric detection: application to enzyme-hydrolysed methyl cellulose
A novel method for detection of reducing ends of sugars is proposed, based on the use of Fe(CN)(6)(3-) as the oxidant in combination with amperometric detection and flow injection analysis (FIA). The method is very sensitive, giving values of < 10 mu M for the limit of detection for a series of mono- and oligosaccharides. Samples can be analysed every 30 s, and injection can be made fully automate
