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Optimization of production of high-quality recombinant antibody fragments for use in microarray applications

Effektivare antikroppsproduktion kan leda till bättre forskningsverktyg och framsteg inom antibody microarray Antikroppen har funnit en plats inom analys av proteinsammansättningar. Forskningsverktyget antibody microarray har i det avseendet visat sig vara kraftfullt och används idag flitigt. Krav på produktionen av antikroppsfragment har således ökat, men att producera dessa har visat sig var utAntibodies have proven its usefulness in a variety of technologies, including Flow Cytometry, Immunohistochemistry and Antibody Microarrays. The latter, which has been applied in e.g. biomarker discovery, disease monitoring, prognostics and protein expression profiling, has become a workhorse in affinity proteomics. By recombinantly expressing scFv antibody fragments in E. coli, a high-throughput

När vardagen förändras för unga vuxna med cancer.

Barncancervården förbättras successivt vilket innebär att överlevnaden ökar bland barn och ungdomar och fler barn uppnår vuxen ålder. Detta leder till att fler unga vuxna övergår från barn- till vuxensjukvården. Barnsjukvården och vuxensjukvården har en stor utmaning i att förbättra övergången mellan enheterna och för att nå ett bra resultat krävs samarbete. Syftet med studien var att belysa hur u

Test av ny mätmetod för att mäta personflöde genom öppningar

The purpose of this study is to evaluate a method of measuring personal flow through door openings using the laser scanner RPLIDAR. An experiment was carried out November 2nd 2016 in the fire laboratory at the Faculty of Engineering, Lund University. This report is limited to finding the potential for future applications based on the experimental data obtained. During the experiment the laser sc

Planerad spontanitet - komposition och plankning som metod för att lära ut improvisation till trummisar

Den här studien undersöker hur egenskrivna och/eller lånade idéer kan användas som metod för att utveckla improvisationsförmågan hos elever på estetiska programmet med trumset som huvudinstrument. Studiens deltagare har efter givna ramar fått i uppgift att planera och sedan framföra egna trumsolo-sekvenser, i syfte att fylla på den egna musikaliska verktygslådan med solistiska verktyg. Den här stuThis study examines how original and/or borrowed ideas can be used as a method for high school students with drum profile to develop and improve their ability to improvise. The students of this study have been instructed to prepare and to play drum solo sequences based on certain limitations, in order to fill their musical toolbox with new solistic tools. This study is based on qualitative intervi

Ambulanssjuksköterskors erfarenheter av att smärtbehandla barn

Studier visar att när barn upplever akut smärta finns det flera olika faktorer som påverkar om barnet får smärtbehandling. Bristande erfarenhet av smärtbehandling av barn och rädsla att tillfoga onödig smärta är några av orsakerna. Syftet med denna studie var att belysa ambulanssjuksköterskors erfarenheter av att smärtbehandla barn. Studien utfördes som en kvalitativ intervjustudie med induktiv an

High speed detecting and identification for car charging on electric roads

The constantly increasing awareness of protecting the environment has put electri- cal roads in the spotlight as an alternative solution to fossil driven means of trans- port. Dan Zethraeus has developed an innovative idea for a prototype electrical road which conductively supplies power to the cars whilst driving. The concept is to place a line of short rail segments in the middle of the drive la

Relationship Between Mindfulness and Decision Making Outcome

Despite the fact that mindfulness has been gaining a lot of interest and is now being researched in different fields to examine the relevance of it to both physical and psychological health, little to almost nothing has been done in relation to everyday decision making. The present study aims to examine the link of mindfulness and decision making, as well as to identify the possible socio-demograp

Examensarbete - Framtagande av handlingsplan för geodatabas

Signal is a very important part of the railway. If you place a signal or a different object which is also important in the wrong place, can mean increased safety risks. To ease the work of the consultants in the creation of operational site drawings, the aim is to develop an action plan for the creation of a database, which in turn will be used to create a tool that can be used to automate the pro

Improvisation inom den klassiska traditionen - en undersökning i hur instrumentlärare inom den klassiska traditionen resonerar kring improvisation

Improvisation och klassisk musik är två begrepp som ofta står i en spänd och lite mystisk relation till varandra. Syftet med denna studie är att undersöka hur musik- och instrumentlärare inom den klassiska genren på högskolenivå resonerar kring improvisation. Studien ska belysa deras uppfattning kring vad improvisation är, hur den kan användas i musicerandet på ett praktiskt sätt och hur man kan a

Gene-ID Using Simultaneous DNA Barcoding and Enzymatic Labeling

Plasmider är korta cirkulära DNA-molekyler, som kan hittas i de flesta bakterier. Detta projekt handlar om att skapa och testa ett nytt sätt att identifiera plasmider, samt specifika gener på dessa. Principen bygger på att kombinera en tidigare metod kallad “Competitive binding-based optical DNA mapping”, och infärgning av en längre DNA-sekvens med fluoroscerande (självlysande) molekyler, för att Antibiotic resistance is an ever growing problem, and is considered one of the major challenges of modern medicine. In order to avoid further development and spread of multiresistance among bacteria, the use of antibiotics should be more restrictive, and more tailored to individual cases of infection. To facilitate more directed treatments, it is crucial to be able to quickly and reliably identify

När innehållet är Word-of-Mouth-smart: En innehållsanalys av svenska B2B-företags mest uppskattade Facebook-inlägg

Denna uppsats analyserar innehållet och den underliggande meningen bakom B2B-företag mest uppskattade Facebook-inlägg. Teorin ger läsaren en beskrivning av den forskning som har gjorts på varumärken och content marketing, följt av teorier om relationsmarknadsföring, varumärkeslöften, varumärkesidentitet och brand communities. I studien används en abduktiv ansats. Genom att använda en kvalitativ meThis thesis analyzes the content and the underlying meaning of B2B companies' most appreciated Facebook posts. The theory gives the reader a description of the research made on brands and content marketing, followed by theories of relationship marketing, brand promises, brand identity and brand communities. The study utilizes an abductive approach. By using a qualitative methodology, a semioti

Visual navigation and control of a mobile robot

Robotar kan programmeras för att självmant röra sig runt i världen. I takt med att kameror blir både billigare och bättre är det vanligt att robotar använder kameror för att se sin omvärld.As cameras become cheaper and better, using them as sensors have become increasingly more common. This is especially true in robotics and for autonomous robot navigation. This thesis suggests a method where inputs from a single camera are used for making a robot self driving. The robot is assumed to move along planar surfaces, common in indoor environments. The method is based on matching image f

Modelling and Control of the Crazyflie Quadrotor for Aggressive and Autonomous Flight by Optical Flow Driven State Estimation

The master thesis seeks to develop a control system for the Crazyflie 2.0 unmanned aerial vehicle to enable aggressive and autonomous flight. For this purpose, different rigid-body models are considered, differing primarily in their parametrisation of rotation. The property of differential flatness is explored and several means of parametrising trajectories in at output space are implemented. A ne

Construed External Image and Organizational Identification in a Virtual Workplace

The current study presents a first step in investigating the relationship between organizational identification and its proposed antecedents: construed external image and perceived work-based social support, in a completely virtual setting. Additionally, the study examines work-based social support as a moderating variable of the relationship between construed external image and organizational ide

The Effect of Architectural Configurations on the Biological Light Response in Residential Buildings

Daylight has beneficial psychological and health effects that range from enhanced alertness, mood improvements, increased productivity, and well-being. Recently, the interest in non-visual (also called biological) effect of light has increased substantially. Yet, a limited number of research has investigated the non-visual effects of light in Nordic climates. The aim of this thesis is to assess th

When censoring anti-immigration attitudes backfires - The impact of reactance and censorship on political attitudes

Anti-immigration movements are currently enjoying electoral success around the world, despite attempts by incumbent parties to mitigate their influence on voters. This seeming paradox is the main focus of this paper, aiming to investigate whether attempts censor and ostracize antiimmigration attitudes actually mitigate their influence or not. Two studies were conducted: Study 1 investigated whethe

Chinese Visitors’ Participation in Culinary Tourism in the Öresund Region

This thesis is an ethnographic study of Chinese visitors’ participation in culinary tourism in the Öresund region. It aims to do qualitative research on the consumption of exotic food and the status of cooking with locals’ activity among Chinese tourists and to deliver insights to culinary tourism agencies in terms of attracting Chinese tourists to joining the food-related activities in present an

Filipina Domestic Workers in São Paulo - An Explorative Case Study on the Social Reproduction of Class, Gender and Race

The study is a first attempt to academically investigate the case of Filipina domestic workers in São Paulo. In brief, it aims to outline these migrants’ life and working conditions, their insertion and function within class relations and in regard to a gendered and racialized work regime. The overarching theoretical framework is social reproduction feminism that originated from the 1960’s sociali

Möjligheter och effekter vid ökad materialåtervinning i offentliga miljöer – En studie med fokus på Lunds gaturum

Konsumtionen i världen ökar och det gör även halten av växthusgaser i atmosfären. Detta kräver högre resurseffektivitet och inom både EU och Sverige finns styrmedel som förespråkar ökad materialåtervinning. Trots detta saknas ofta källsorteringsmöjligheter i offentliga miljöer. I denna studie undersöks möjligheterna till ökad materialåtervinning i offentliga miljöer genom att fokusera på ett införThe global consumption is increasing and so are the levels of greenhouse gases in the atmosphere. This requires a higher resource efficiency and legislation within the EU and Sweden is promoting material recycling to a greater degree than incineration of waste. In spite of this recycling stations can rarely be found in public environments. In this study the possibilities and effects of an increase

Feasibility study of thermochromic inks for the packaging industry

A thermochromic ink is defined as an ink that exhibits colour changes depending on temperature. Thermochromic inks used in printing generally consist of an encapsulated three part system which includes a dye that changes colour, a compound that acts as a colour developer and a solvent. Due to the properties of the dye, the inks are always colourless above a certain temperature and coloured below a